Establishment of tissue cultures, Biology

Establishment of Tissue Cultures

By now you are familiar with the term "Explants". After a few days in culture the explant becomes slightly rough in texture and its surface glistens in reflected light. This is a sign of the beginning of callus formation. A callus is defined as an irregular tissue mass varying considerably in texture, appearance and growth rate. In nature plants produce callus as a result of mechanical injury (wound tissue), invasion by microorganisms or by insect feeding. Callus formation has been observed in angiosperms, gymnosperms, ferns, mosses and liverworts. Infection by Agrobacterium tumefaciens causes the production of tumors or 'Crown gall' in dicotyledonous plants. The stimulus for cell proliferation in these cases is provided by endogenous hormones, auxins and cytokinins. Plant material typically cultured included vascular cambia, storage parenchyma, pericycle of roots, cotyledons, leaf mesophyll and provascular tissue. You should know that all multicellular plants are potential sources of explants for callus initiation. In the laboratory you can easily establish callus cultures from the stem pith tissues of tobacco or slices of carrot root on MS medium supplemented with 2, 4-D.

Posted Date: 1/24/2013 5:03:21 AM | Location : United States







Related Discussions:- Establishment of tissue cultures, Assignment Help, Ask Question on Establishment of tissue cultures, Get Answer, Expert's Help, Establishment of tissue cultures Discussions

Write discussion on Establishment of tissue cultures
Your posts are moderated
Related Questions
Q. Dietary Fibre requirement during diabetes? Dietary fibre is that part of food which is not digested by the intestines. High fibre meals have shown to give the best glycemic

Which of the following is not true regarding the complement system?Answer This system involves a group of at least 20 proteins found in the blood plasma. During an infection, one p

Phylogenetic or Cladistic Classification Phylogeny plays a great role in classification. It is the  appropriate theoretical background for taxonomy and is quite essential in exp

Q. Does thermal inversion occur in the winter or in the summer? The Pollutant low altitude thermal inversion take place in the winter and in this period of the year the sun hea

What is pleiotropy? Pleiotropy (or pliotropy) is the occurrence in which a single gene conditions several dissimilar phenotypical traits. Some phenotypical traits might be s

PHYSIOLOG Y OF DIGESTION Food - omnivorous. Digestion - Intercellular. (i) Mechanical - by chewing, deglutinationm, churnning, peristalais. Under control of medulla oblonge

VERTEBR A T A It is divided into two parts -        1. Agnetha                    2. Gnetha S.No. C haracter A gnetha G

Cleavage - Development Biology Cleavage or segmentation is a series of cell divisions of the fertilized .egg through which it is converted into a multicellular structure, call

Q. What is Working Memory? Working Memory: It has often been suggested that memory does not always work in three stage sequence and the model of memory system comprising of sen

What is Eutrophication in ecology explain briefly? Freshwater ecosystems demonstrate the close relationship between aquatic and terrestrial ecosystems. Until recently, most peo