Establishment of tissue cultures, Biology

Establishment of Tissue Cultures

By now you are familiar with the term "Explants". After a few days in culture the explant becomes slightly rough in texture and its surface glistens in reflected light. This is a sign of the beginning of callus formation. A callus is defined as an irregular tissue mass varying considerably in texture, appearance and growth rate. In nature plants produce callus as a result of mechanical injury (wound tissue), invasion by microorganisms or by insect feeding. Callus formation has been observed in angiosperms, gymnosperms, ferns, mosses and liverworts. Infection by Agrobacterium tumefaciens causes the production of tumors or 'Crown gall' in dicotyledonous plants. The stimulus for cell proliferation in these cases is provided by endogenous hormones, auxins and cytokinins. Plant material typically cultured included vascular cambia, storage parenchyma, pericycle of roots, cotyledons, leaf mesophyll and provascular tissue. You should know that all multicellular plants are potential sources of explants for callus initiation. In the laboratory you can easily establish callus cultures from the stem pith tissues of tobacco or slices of carrot root on MS medium supplemented with 2, 4-D.

Posted Date: 1/24/2013 5:03:21 AM | Location : United States







Related Discussions:- Establishment of tissue cultures, Assignment Help, Ask Question on Establishment of tissue cultures, Get Answer, Expert's Help, Establishment of tissue cultures Discussions

Write discussion on Establishment of tissue cultures
Your posts are moderated
Related Questions
Define Role in Glucose, Lipid and Nucleic Acid Metabolism? Chromium leads to a decrease in blood glucose concentration in people with elevated glucose levels and an increase in

Define Regulation of amino acid levels in the blood? Regulation of amino acid levels in the blood: Vitamin D helps to prevent lossĀ  of amino acids through the kidney and thus

The major hazards encountered in the biological lab work are diseases like infections and allergies which are caused by handling live animals. dissections, plant and animal tissues

Which of the following processes in capillaries in the lung assist in the removal of carbon dioxide from the body? A. Net flux of bicarbonate from plasma into red blood cells.

Explain how early RNA molecules might have been able to respond to natural selection. Every RNA molecule might have competed with slightly dissimilar RNA molecules for nucleoti

Explain Changing Body Composition with Ageing? With ageing, a progressive decline in the water content and the lean body mass is accompanied by an increasing proportion of body

Q. Do enzymes act better under basic or acid pH? Most enzymes act in pH between 6 and 8, a range that corresponds to the general acidic level of blood and cells. There are enzy

Induction of Mesoderm in Amphibians Earlier you know that an event that precedes neural ectoderm induction in amphibians is the induction of mesoderm. Until 32 cell stage in

Define Proteins required for underweight - Nutritional Care? Proteins are required for tissue building, as well as, to take care of the daily wear and tear. Under weight indivi

Q. What is Constipation? Constipation is irregular, infrequent or difficult passage of faeces. It is the most common physiological disorder of the alimentary tract. It is chara