Define role of buffer in electrophoresis, Biology

Define Role of Buffer in Electrophoresis?

Buffer ions have a two-fold purpose in electrophoresis, they carry the applied current, and they fix the pH at which electrophoresis is carried out and, thus, determine the kind of electrical charge on the solute and the extent of ionization of the solute. By these effects, the buffer determines the direction of the electrophoretic migration to one of the two electrodes.

The buffer ionic strength determines the thickness of the ionic cloud surrounding the charged molecules and, thus, also the rate of migration and the sharpness of the zones. Many buffer systems are used in electrophoretic procedures, but the most widely used are the barbital buffers and the Tris-boric acid EDTA buffers. Next, let us get to know about the different techniques of electrophoresis.

Posted Date: 6/15/2013 6:58:02 AM | Location : United States

Related Discussions:- Define role of buffer in electrophoresis, Assignment Help, Ask Question on Define role of buffer in electrophoresis, Get Answer, Expert's Help, Define role of buffer in electrophoresis Discussions

Write discussion on Define role of buffer in electrophoresis
Your posts are moderated
Related Questions
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

GENETICS 1.         The branch genetics started in 1900 after rediscovery of Mendel's work. 2.         Gregor Johann Mendel is called Father of genetics. 3.         The t

Define Water soluble Vitamins - B complex Vitamins and Vitamin C? Vitamins are essential nutrients found in foods. The requirements are small but they perform specific and vit

How would carbon monoxide poisoning affect a person's coloring, particularly of the mucous membranes? How would it affect the hemoglobin concentration, hematocrit, and percent oxyh

how is biotechnology useful?

SPINAL NERVES 31 Pairs. Mixed type. Total weight 150 gms. At the base of origin of spinal nerves gland of swammardon or calcarious ganglion is present. Composed of m

What are the respective constituents of the gray matter and of the white matter of the spinal cord? The gray matter, or gray substance, of the spinal cord having predominantly

Define briefly about the Seed Proteins? Although a large number of plants produce seeds having protein contents in excess of 15%, only a few are utilized for food, eg. soybean,

Q. Does the DNA replication occur in the cell division? Yes. The DNA replication occurs in mitosis as well in the meiosis.

what are the two alternate pathways to glycolysis