Define procedure for detection of number of bacteria in milk, Biology

Assignment Help:

Define Procedure for Detection of Number of Bacteria in Milk?

1. Place a clean, non-greasy slide over the graph paper.

2. Spread 0.01 ml of the milk over an area of 1 cm2 as a thin even film.

3. Dry the smear by gently passing through the flame.

4. Defat the smear by flooding with xylol for 1 minute.

5. Wash the slide with alcohol to remove xylene followed by washing with water to remove the alcohol.

6. Stain the smear with methylene blue for 15-30 second.

7. After washing with water, observe the dried preparation under oil immersion. Count the number of bacteria in about 25 microscopic field.

8. Determine the average number of bacteria per microscopic field and multiply with microscopic factor (500,000) to get bacterial counts per ml of the milk sample.


Related Discussions:- Define procedure for detection of number of bacteria in milk

Define indicators at the individual level, Define Indicators at the individ...

Define Indicators at the individual level? Number of individuals who have gone hungry through lack of personal food supply, amount of expenditure on food, percent of disposable

Counselling skills used in diabetes mellitus, Q. Counselling skills used in...

Q. Counselling skills used in diabetes mellitus? Information gathering is an important skill for understanding of the client. Behaviour is complex and, therefore it is importan

Show the probability to predict the ratios of progeny, Use the rules of pro...

Use the rules of probability to predict the ratios of progeny expected from two parents who are heterozygous for albinism. Note: Albinism is recessive to normal skin color. 1) What

Male reproductive system - accessory glands, ACCESSORY GLANDS - 1 .  ...

ACCESSORY GLANDS - 1 .      PROSTATE GLAND - One in number, 4-5 lobed, it forms 15-30% of semen.Citric acid, lipid, acid phosphatase spermin, fibrinolysine are present.

Gastrulation in chick, Gastrulation in Chick Cleavage in the fertilize...

Gastrulation in Chick Cleavage in the fertilized egg occurs during its passage through the oviduct to cloaca of the hen. By the time it is laid meroblastic cleavage has result

Explain what is fungi, Explain what is Fungi? The fungi are spore-beari...

Explain what is Fungi? The fungi are spore-bearing eukaryotic organisms without chlorophyll and having absorptive nutrition. These reproduce sexually as well asexually. Primari

Unit 4 Assignment: The Complexity of Human Organs, Ask quYour organs are wo...

Ask quYour organs are wonderous things, each one with a different function vital to the homeostasis of your body. While it is easy for us to view a particular organ as a single ite

Determine the use of natural colourants, Determine the use of natural colou...

Determine the use of natural colourants The use of natural colourants is limited due to their instability, low tinctorial power or price disadvantage.  The trend towards natura

Explain adverse effects of foscarnet, Explain Adverse Effects of foscarnet ...

Explain Adverse Effects of foscarnet  Renal dysfunction often develops during treatment with foscarnet and is usually reversible, but renal failure requiring dialy sis may occu

Excretion, How will the urine concentration be affected if the henle''s loo...

How will the urine concentration be affected if the henle''s loop of a person is removed and his PCT is joined directly with DCT?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd