Coagulation - blood collection , Biology

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 

Posted Date: 10/15/2012 5:30:03 AM | Location : United States







Related Discussions:- Coagulation - blood collection , Assignment Help, Ask Question on Coagulation - blood collection , Get Answer, Expert's Help, Coagulation - blood collection Discussions

Write discussion on Coagulation - blood collection
Your posts are moderated
Related Questions
Cost due to Damage to Equipment Cost due to Damage to Equipment, Property etc. Tooling may have to be redesigned, adding cost. Cost due to reduced output and delay

DISORDERS OF LOWER RESPIRATORY TRACT: The lower respiratory  tract infections  tend to occur most frequently in young children who have not developed resistance  to infectious


difference between axon and cyton

Chagas' Cardiomyopathy It is common in south and Central America and is caused by Trypanosoma cruzi. Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) It is mark

How is the genetic determination of sex established in humans? In the diploid genome of the human beings, there are 46 chromosomes, 44 of them are autosomes and two are sex chr

Give the introduction to Congenital Heart Disease ? The past 3 or 4 decades have witnessed a revolution in pediatric cardiac sciences. A number of conditions previously conside

S e l e n i u m Selenium toxicity due to pollution of environment has been reported in animals. Contamination of pasture from industries emitting fly ash particularly fo

difference between one girl and lungs two ribosomes and lysosomes ?

Pre-operative Teaching Brief explanation-anatomy, physiology of the cardio-respiratory system.  The disease and the operation which is going to be done.  Operati