Cloning of arg3 gene, Biology

1)  You want to clone the arg3 gene, which encodes an enzyme in the arginine biosynthetic pathway in Neurospora crassa.  You have available the E. coli plasmid vector shown in part c below, a tube of Neurospora genomic DNA, a tube of Neurospora cDNA, and a method of introducing recombinant DNA samples into Neurospora.

 a)  Which would you prepare to try to rescue the arg3- mutant phenotype by transformation rescue?  Circle one.  

  a)  Genomic DNA library completely digested by your restriction enzyme of choice

  b)  A genomic DNA library partially digested by your restriction enzyme of choice

  c)  A cDNA library  

b)  Briefly explain your answer.

c)  The plasmid below has an origin of replication (ori), a gene that confers resistance to streptomycin (strR), and the lacZ gene which converts X-gal to a blue compound.  All of the sites for the restriction enzymes HpaII, EcoRI, HindIII, PstI, and SmaI are shown on the plasmid.  Which restriction enzyme would you cut the vector with to make your library?  

  a)  HpaII

  b)  EcoRI

  c)  HindIII

  d)  PstI

  e)  SmaI

d)  Using the source of DNA you chose in part a and the enzyme you chose in part c, list the steps you would follow to generate recombinant DNA molecules, get them into bacterial host cells (should these bacteria be resistant or sensitive to strep? should the bacteria have a functional lacZ gene?), identify those bacteria containing vector molecules with inserts, and then use transformation rescue to figure out which recombinant DNA molecules contain the arg3 gene.  

 

 

Posted Date: 2/27/2013 6:10:17 AM | Location : United States







Related Discussions:- Cloning of arg3 gene, Assignment Help, Ask Question on Cloning of arg3 gene, Get Answer, Expert's Help, Cloning of arg3 gene Discussions

Write discussion on Cloning of arg3 gene
Your posts are moderated
Related Questions
GONADS - They develop from the mesoderm of the embryo. The gonads are the sex glands; the ovaries and the testes. They produce ova and sperms respectively i.e. cytogenic in

Q. How is the extracorporeal digestion associated to predation in arachnids? Arachnids can inoculate poison to kill or paralyze their preys using structures called chelicerae.

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Procedure for Preparation of Selective and Differential Medium Now prepare the MacConkey agar medium following the steps given herewith: 1. Weigh and dissolve all ingredient

Results of CABG: In most centres where this operation is routinely conducted the mortality is 1-2 per cent. In high-risk group of patients this may go up to 5 to 10 per cent.

Define Feeding and Nutritional Management for patient of alzheimer's disease? Keeping the clinical manifestations of Alzheimer's disease in mind, treatment involves personaliz

Food Chain - Ecosystem In a food chain, the food energy is transformed from a given source through a series of species, each of which eats the one before itself in the chain.

Bioinert Metals:-  Commercially pure titanium/ titanium alloys Ceramics:-  Aluminum oxide, zirconium oxide

what is the difination of osmosis

Define meal planning and nutrient needs of fast-growing infants? We just got to know the meal planning and nutrient needs of fast-growing infants and preschoolers in the previo