Carbon dioxide for photosynthesis, Biology

Aim: To prove that Carbon dioxide is essential for photosynthesis.
Apparatus: A potted healthy plant with long and narrow leaves, a wide mouthed glass bottle, split cork.
Chemicals: Potassium Hydroxide solution (KOH), vaseline, Iodine solution.
Procedure :
1) Select a healthy potted plant with long and narrow leaves and keep it in darkness for two or three days so that leaves are free from starch. Take a wide mouthed glass bottle, a cork.
2) Take 5-10ml of potassium hydroxide solution into the bottle.
3) Close the mouth of the bottle with split cork.
4) Early in the morning insert one selected half of the leaf without cutting it from the plant, into the bottle through the split cork.
5) The other half of the leaf is outside the cork.
6) The bottle is kept air tight by applying vaseline around the rim of the mouth of the bottle.
7) Keep this apparatus in sun light for 3-4 hours. After the completion of time, detach the leaf from the plant and take it out of the bottle.
8) Test the detached leaf for starch test with iodine solution.
Observation : The part of the leaf which is inside the bottle does not turn deep blue, while the basal part of the leaf that is exposed to surroundings prepares starch and turns deep blue.
Inference : The potassium Hydroxide absorbs the carbon dioxide present inside the bottle hence the leaf inside the bottle does not turn deep blue, while the basal part of the leaf that is exposed to surroundings prepares starch and turns deep blue.

Conclusion : Green leaves can perform photosynthesis only when Carbon dioxide is available. Hence carbon dioxide is essential for photosynthesis.
Precautions :
1. The bottle should be kept airtight by applying vaseline around the rim of the mouth of the bottle.
2. Make sure that potassium hydroxide does not come in contact with leaf inside the bottle.
3. The leaf should not be detached from the plant till the experiment is completed.

1175_Carbon dioxide is essential for photosynthesis.png

Posted Date: 8/29/2012 8:24:32 AM | Location : United States







Related Discussions:- Carbon dioxide for photosynthesis, Assignment Help, Ask Question on Carbon dioxide for photosynthesis, Get Answer, Expert's Help, Carbon dioxide for photosynthesis Discussions

Write discussion on Carbon dioxide for photosynthesis
Your posts are moderated
Related Questions
what is the main quality of this phylum

Q. How do ascaris obtain food? An ascaris live within the human gut and feed from the food ingested by the infected person.

Define Functions of sodium and chloride? So far you have learnt that most minerals participate in important functions of body as they support the activity of specific enzymes.

Post-partum anoestrus Reproductive efficiency among animals greatly depends upon detection of estrus. This is even more important in reference to small herds managed under tro

Body Musculature - Locomotion The layout of the muscle layers surrounding the coelom is necessarily the same in all the major classes of Annelida. The body wall has a layer of

Name the one autosomal dominant and autosomal recessive Mendel an disorder in Humans. a) How is the action of exonucluease dissimilar from that of end nuclease? b) India has

Risk during crown removal -Tooth inadvertently extracted using a crown\bridge remover . -Endodontic therapy was performed , and the tooth was replanted , a procedure known

Q. What are pericardium, pleura and peritoneum? Pleura are the membrane that covers the inner wall and the lungs of the chest pericardium are the membrane that covers the heart

Q. Define Hepatic Cholesterol Synthesis? This is a highly complex process beginning with acetyl CoA formed from fatty acid oxidation or from carbohydrate breakdown. The rate-de

Determine the Fransworth 100 Hue Panel Test This is a simple and useful test using colour chips arranged in a particular sequence. Colour deficient patients make errors in arra