Blotting, Biology

Blotting is the technique for detecting one RNA within the mixture of RNAs (a Northern blot) or one type of the DNA within of a mixture of DNAs (a Southern blot). A blot can prove whether that one species of RNA or DNA is present, how much is there, and its estimated size. Mainly, blotting involves gel electrophoresis, transfer to the blotting membrane (typically nitrocellulose or activated nylon), and incubating with the radioactive probe. Exposing membrane to X-ray film creates darkening at the spot correlating with the position of the DNA or RNA of interest. The darker is the spot, the more nucleic acid was present there.

Posted Date: 8/1/2012 7:12:52 AM | Location : United States







Related Discussions:- Blotting, Assignment Help, Ask Question on Blotting, Get Answer, Expert's Help, Blotting Discussions

Write discussion on Blotting
Your posts are moderated
Related Questions
Ammonification Many heterotrophic bacteria actinomycetes and fungi in soil and water metabolise the organic nitrogen and release it in an inorganic form as ammonia. This proce

Factors Controlling Stomatal Aperture 'Khul Ja Sim Sim' the two rocks slide and the gates to a great treasure open. With these magic words "Ali Baba and Chalis Chor" could ope

Describe Class Holothuroidea in details? Members of this Class resemble soft and squishy cucumbers lying on their sides on the bottom of the sea. On first glance, they appear n

Epimerism:- Another type of stereo isomerism known as epimerism occurs with respect to a single asymmetric carbon atom of a monosaccharide possessing more than one asymmetric

what are some technologys used in the integumentaryy systems

Systemic Infection Serious infections increase total body metabolism and thus impose hemodynamic burden on the heart. Increased heart rate associated with infections is an addit

Why do the sons of a haemophiliac father never vary from this trait? Why is the cleavage in mammals referred as simple homoplastic?

Use the rules of probability to predict the ratios of progeny expected from two parents who are heterozygous for albinism. Note: Albinism is recessive to normal skin color. 1) What

Light - Marine Ecosystems Light is a limiting factor in the ocean as it contributes significantly to organic production and distribution of marine life. The ocean is divisible

COMMO N EYE DEFECTS - 1 .       Glaucoma - Pressure of aquous humour is increased, retina is degenerated. 2 .       Presbyopia - Due to non etastic cilliary muscles,