Blotting, Biology

Blotting is the technique for detecting one RNA within the mixture of RNAs (a Northern blot) or one type of the DNA within of a mixture of DNAs (a Southern blot). A blot can prove whether that one species of RNA or DNA is present, how much is there, and its estimated size. Mainly, blotting involves gel electrophoresis, transfer to the blotting membrane (typically nitrocellulose or activated nylon), and incubating with the radioactive probe. Exposing membrane to X-ray film creates darkening at the spot correlating with the position of the DNA or RNA of interest. The darker is the spot, the more nucleic acid was present there.

Posted Date: 8/1/2012 7:12:52 AM | Location : United States







Related Discussions:- Blotting, Assignment Help, Ask Question on Blotting, Get Answer, Expert's Help, Blotting Discussions

Write discussion on Blotting
Your posts are moderated
Related Questions
Q. What are the secondary and the primary constrictions of a chromosome? And what is the other name given to the secondary constriction? Primary constriction is the narrower re

Problem 1: How do you differentiate between a dossier and a submission document? Define dossier Define submission document differentiate between a dossier and a

Thalassemia   Thalassemias are the commonest hemolytic anaemias or disorder seen in children in India. This is characterised by deficiency  in the synthesis  of one of the norm

EPIDIDYMIS - All seminiferous tubules form short straight tubules as tubuli recti, open into wider network of rete testis. From rete testis 15-20 fine convoluted tubules,

In fact a real earthquake ground motion at a particular site is much more complicated that than the simple waveform depicted. It would be beneficial if we make comparison between t

Define SEPSIS - Nutrition during Stress? Sepsis is defined as the presence of an infection due to an identifiable organism. Bacteria and their toxins lead to a strong inflammat

Q: What are disaccharides? Although theoretically disaccharides could be made up of two trioses, tetroses, pentoses but all naturally occurring disaccharides contain hexose mol

Express the term ctene. This unique locomotory structure is found in Ctenophora and is made up of fused cilia arranged into flattened plates. Ctenes are then organized into eig

Describe Class Holothuroidea in details? Members of this Class resemble soft and squishy cucumbers lying on their sides on the bottom of the sea. On first glance, they appear n

Q What is the mass transportation across the cell membrane? Mass transportation is the exiting or the entrance of substances in or from the cell engulfed by portions of membran