Question about molecular biology

Assignment Help Biology
Reference no: EM1386472

Assume you infected an E. coli culture with a virulent bacteriophage. Most of the cells lysed, but a few survived - 1x10^-4. You wonder where the resistant bacteria came from. Were they caused by the bacteriophage infection or did they already exist in the bacterial culture? Earlier, for a different experiment, you ha spread a dilute suspension of E. coli onto solid medium in a large Petri dish, and, after seeing that about 10^5 colonies were growing up, you made an imprint of the colonies on that plate and transferred it to three other plates - a process known as replica plating - which creates identical patterns of the colonies on 3 plates. You realize that you can use these plates to distinguish between the two possibilities. You pipette a suspension of the bacteriophage onto each of the 3 replica plates so that the bacteria can be infected. What result do you expect if the bacteriophage cause resistance? What result do you expect if the resistant bacteria pre-exist?

Reference no: EM1386472

Questions Cloud

How many cars would you expect to see in the system : How many cars would you expect to see in the system. A toll tunnel has decided to experiment with the use of a debit card for the collection of tolls. Initially, only one lane will be used
What torque is required to the motor : A 24.00 kg child is on a swing that hangs from 1.6-m long chains. What is her highest speed if she swings out to a 45° angle.
Find running time of heap sort input sorted-ascending order : Determine the running time of Heap Sort if input is sorted in ascending order. Determine the running time of Heap Sort if input is sorted in descending order.
Formulate a spreadsheet model that will give the profit : Formulate a spreadsheet model that will give the profit in part b for any of the two numbers. Use the Excel goal seek command to calculate the break-even point found in part a.
Question about molecular biology : Assume you infected an E. coli culture with a virulent bacteriophage. Most of the cells lysed, but a few survived - 1x10^-4. You wonder where the resistant bacteria came from.
Determine the amount by which the spring is stretched : A 9.10 kg board is wedged into a corner and held by a spring at a 50° angle, as the Illustrating shows. The spring has a spring constant of 196 N/m and is parallel to the floor.
Determine the mode shapes and frequencies of vibration : Determine the mode shapes and frequencies of vibration of the system of cars by Sweeping the rigid body mode
The objective is to find a production plan that maximizes : The objective is to find a production plan that maximizes the daily profit. Formulate your model mathematically and solve it by using the Excel solve
Define the average force exerted on the ball : While a certain rubber band is stretched a distance x, it exerts a restoring force of magnitude F=ax+bx^2, where a and b are constants. The work completed in stretching this rubber band from x=0 to x=L is what.

Reviews

Write a Review

Biology Questions & Answers

  Immune response to infectious disease

It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.

  A review on advanced glycated end products (ages)

This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.

  Plastic as a soil stabilizer

Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.

  Principles of microbiology

This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.

  List the biologic functions

Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?

  Biologic function relationships

Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.

  Case study on patient in the haematology laboratory

Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.

  Use of pcr and genetic approaches in biotechnology

The use of PCR and genetic approaches in biotechnology

  Describe the role of this enzyme in honey

Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.

  Genetic problems

What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?

  Prepare an essay on nosocomial infection

Prepare an essay on nosocomial infection.

  Monitoring and recording the blood pressure

To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd