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Provided the rising rates of hypercholesterolemia and hypertension in the U.S. and other industrialized nations, you are interested in studying the importance of cholesterol transport in the sodium re-absorptive pathways in the kidney that are important for blood pressure control. One way to study this pathway is to use a mouse model in which an important transporter for cholesterol is "knocked out."Standard gene targeting methods to "knock out" the cholesterol transporter, Abca1, create mice with several severe developmental abnormalities including glomerulonephritis, a kidney defect that would confound the study of other renal processes in this model. For this reason, you decided to make a tissue specific knockout mouse where Abca1 is only "knocked out" in your cell type of interest, the renal collecting duct principal cell. Luckily, you discover a commercially available, floxed Abca1 transgenic mouse model and a Cre-recombinase expressing mouse with the Cre gene downstream of the promoter for the water channel that is expressed in collecting duct principal cells (Aqp2).Questions:1) Describe the Cre/Lox system used to generate tissue-specific knockout mice.2) Reading about the Aqp2-Cre mouse model, you discover that the Aqp2 promoter also causes Cre expression in the vas deferens and testes of male mice. Since these tissues also express Abca1, you desire to avoid mating Aqp2-Cre males as they are infertile. Design a breeding scheme (hint: use Punnett squares) that will produce Aqp2-Cre and floxed Abca1 mice without breeding Aqp2-Cre expressing male mice. (Remember the Cre recombinase gene only needs to be present on one chromosome to function.)What are the ratios of different genotypes that this breeding scheme will produce?3) In question 2 above, which genotypes would serve as your controls for future experiments? Why?
According to your understanding of the nitrate reductase and nitrite reductase tests, why would the inclusion of nitrite inhibit anaerobic growth?
A man and woman are both of normal pigmentation and have a one child out of three who is albino (without melanin pigmentation). Albinism is an autosomal (not sex-linked) recessive trait. What are the genotypes of albino's parents.
What are the steps in isolating the DNA from strawberries. What are the materials required to isolate DNA.
Determine what makes a molecule a hydrogen bond donor or an acceptor? Can a hydrogen on a carbon atom be a hydrogen bond donor? Explain your answer.
What do you think would happen if a person had a mutation that prevented the jaw part of their HLA molecules from becoming the correct shape.
DNA sequences are used quite often to evolutionary relationships between species. Explain why is the DNA sequence of one gene a less accurate indicator of the evolutionary.
The population of two hundred individuals goes through a generation of reproduction in which all HWE assumptions are met except for finite population size.
The U.S government has placed this strain of C.taxifolia on noxious weed list, which means that any possible source of the contamination of the weed will be highly restricted. Shipments that contain any kind of C.taxifolia and pass through an area wh..
What is the possibility that their first child will have attached earlobes. which among the conditions or substances do ALL archaeans need for survival and reproduction.
The ATP solution used in this experiment had a concentration of 0.25 percent, which represents the normal physiological cytoplasmic concentration of ATP.
Black furin mice (B) is dominant to brown fur. short tail is dominant to long tail (t). Short whiskers (W) are dominant to long whiskers.
Assume 2-students are doing a poster on the Amazon Water Lily for a class project and have compiled research to determine if it is in actuality a land plant or an algae. Discuss difference between monoecy and dioecy
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