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Each of the following statements describes a type of column chromatography. Sort the statements to the type of chromatography they describe. If a statement can describe all of the types, place that statement in the "All" category. (Note that size-exclusion chromatography may also be called gel-filtration or molecular exclusion chromatography.)
Categories: Size-exclusion Chromatography, Affinity Chromatography, Ion-exchange Chromatogrpahy, and All Statements:
Separate molecules by size.
Separate molecules by charge.
The stationary phase has a covalently bound group to which a protein in the mobile phase can bind.
Uses a mobile phase and a stationary phase to separate proteins.
The stationary phase contains cross-linked polymers with different pore sizes.
Can separate molecules based on protein-lignand binding.
The stationary phase may contain negatively or positively charged groups.
How could you demonstrate experimentally that the selective antagonist binds the receptor specifically? Illustrate the experiment you would perform and also the controls you would contain.
In metabolic processes of cell respiration and photosynthesis, prosthetic groups as in heme and iron-sulfur complexes are encountered. What do they do.
Pneumonia is diagnosed by a presence of fluid (dark shadows in an X-ray image) in the alveoli. Given that pneumonia generally is caused by a microorganism, what causes the fluid accumulation? Name a bacterium, a virus, a fungus, a protozoan, and a he..
define how the axonal membrane potential is restored. Describe how resting ionic distributions are maintained.
Illustrate what is meant by motivation. What types of non-financial reward might a company employ to motivate employees.
The "Primordial soup" includes the mixture of elements and gases which are thought to have started life on the Earth. Based upon what you have learned related to the cells and complex molecules, do you feel this theory makes sense?
How several 25 ml vials can they fill. An Eon of time is defined as 1 billion years (1x10^9 years). How long is this in seconds? Assume one year is equal to 365.25 days.
Determine volume of the sodium citrate in order to make up 7.5% solution. A solution needs 250 ml of a 7.5% (w/v) solution of the tri sodium citrate.
Approximately what percentage of the energy of glucose is transferred to storage in ATP as the result of the complete oxidation of glucose to CO and water in cellular respiration.
Select the viruses below that would be able to replicate if you injected only their genome into a permissive cell line (Hint: You should be able to answer this question by looking up the Baltimore classificaton of each virus)?
In winter, plants exchange the saturated lipids in their membranes for unsaturated lipids. Unsaturated lipids are "bent" and keep the membranes more fluid since they cannot be stacked closely together. Of what benefit would this be for herbaceous pla..
How is MPF involved in one or more of checkpoints. A chemist wishes to create an organic molecule non-acidic.
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