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O-acetyl serine has the following structure:
O O II IIC---C---O---C---C---C---OH I NH2 It can can be hydrolyzed to acetic acid and serine by O-acetyl serine acetylase.
A reactio mixture containing 20 mM O-acetly serine, 50 mM NaCl, 50 mM HEPES buffer (pH 7.6), and O-acetyl serine is incubated at 37 C until the O-acetyl serine is completely hydrolyzed. What is the pH of the mixture at the end of the reaction? (For O-acetyl serine, pKa1= 1.9, pKa2= 8.6; for serine, pKa1= 2.2, pKa2= 9.2; for acetic acid pKa1= 4.76; for HEPES, pKa1=3, pKa2= 7.6)
Means of transport would most probable be used for moving a medium-sized molecule (like a monosaccharide or an amino acid) from a low concentration on the outside of a cell to a high concentration on the inside of a cell.
Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase.
Discuss how medical technologies are evaluated. Give examples of at least two evaluation methods that have been discussed either in popular press or scholarly journals.
Determine how much in mL of .5 M NaOH total can you add to the solution in question 4 and still have a good buffer also Extimate the pH of a 0.05 M solution of TRIS acid (pKa=8.3)?
What are the targets and mechanisms of action of antiviral drugs to influenza and why are there so few antivirals to influenza available?
Provide an instance where this definition does not apply and in brief explain why this example does not apply to the species concept.
If humans and shrews shared the common ancestor roughly 80 million years ago, ignoring multiple substitutions at the same site, how several amino acid differences have to be expecting to see between a human and shrew Fibrinogen B sequence.
In Drosophila a cross was made between a yellow-bodied male with vestigial (not fully developed) wings and a wild-type female (brown body). The F1 generation consisted of wild-type males and wild-type females.
The matrix in an experiment has a fractionation range of 20000 to 5x10^6 Daltons. Would you see this matrix purify plasmid that had molecular weight of 6x10^6
When digested DNA is analysed using agarose gel electrophoresis the total size of fragments sometimes does not add up to the size of the original DNA molecule. How can this be explained and how can this be seen in ethidium bromide stained agarose ..
select a chromosomal disorder from the March of Dimes Foundation Website.
My favorite bird is the black crowned night heron. The relative proportion of males to females is hypothesized to vary with season.
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