Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Question: Ligation of the multiple ? fragments and pBLU produces a number of different hybrid molecules, although not as many as you might expect. Each clonable fragment must have one BamHI and one HindIII end, to combine with the BamHI and HindIII ends of the pBLU backbone and form a circular recombinant plasmid. Only 5 of the 13 BamHI/HindIII framgnets from the linear ? genome satisfy this requirement: 493 bp, 784 bp, 2,396 bp, 2,409 bp, and 4,148 bp. A sixth clonable fragment of 9,866 bp is derived from the COS circle. Multiple copies of a single fragment or combinations of two different ? fragments can also be ligated into a single pBLU backbone. Three fragment plasmids can be constructed by combining the pBLU backbone with one of hte five fragments having one BamHI end and one HindIII end and with one of the eight fragments having two BamHI or two HindIII ends. Constructs with more than one unit of pBLU are also formed. Oly constructs with at least one unit of pBLU backbone contain an origin necessary for replication and the ampicillin-resistance gene required for growth on selective media.
1. Make scale drawings of all potential two-fragment (vector + insert) recombinant plasmids. Include the total bp size, fragment bp sizes, and the locations of BamHI sites and HindIII sites, the origin, the amplicillin-resistance gene, and the lacZ gene.
Read the review entitled "RNA Virus Replication Complexes" by Tao & Ye. Choose one of the following: (+) RNA Viruses, (-) RNA viruses or dsRNA virus and describe the location in the cell of its replication complex and the mechanism by which replicati..
What part of the sewage treatment plant deals with insoluble and complex organic wastes? Which molecule contains more energy, X or Y.
Here are the distances between adjacent pairs of loci. A map distance of fifty = independent assortment. Please map genes giving distances between adjacent pairs of loci.
Chlamydias and rekettsias were once classified as viruses because of their small size and obligate intracellular growth requirement. What are some of techniques can be used to illustrate that they are really bacteria.
There is a population of 100 frogs. Twenty are heterozygotes, 30 are homozygous recessive, 25 are homozygous dominate. What is the frequency of the dominate allele?
define What would the genotypes and phenotypes. Follow an X-linked recessive mutation from a cross between a heterozygous female and a wild-type male.
Imagine that the sample of sediments are cemented together to form a sedimentary rock. After that, that rock is heated and compressed into a metamorphic rock.
You are conducting field work and discover two groups of squirrels living on opposite sides of a lake. How would you plan a study to find out whether these two groups belong to the same species.
Explain how the potato cylinders changed in their turgidity. Recognize which of the solutions were isotonic, hypertonic, and hypotonic in relation to the potato.
Suppose that the length of ten base pairs of DNA found in one turn of the helix is 34 A, what is the average length of a human chromosome in centimeters?
The binomial name of an organism consists of the names of which two classification categories. Smoking, air pollution and altitude all affect the rate of respiration. Explain the effects of each of these on respiration and Why.
How does this neurotoxin affect the nervous system's ability to stimulate skeletal muscle contraction? How does it affect the capability of skeletal muscle fibers to respond to stimulation.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd