Creating recombinant plasmids

Assignment Help Biology
Reference no: EM1387491

Question: Ligation of the multiple ? fragments and pBLU produces a number of different hybrid molecules, although not as many as you might expect. Each clonable fragment must have one BamHI and one HindIII end, to combine with the BamHI and HindIII ends of the pBLU backbone and form a circular recombinant plasmid. Only 5 of the 13 BamHI/HindIII framgnets from the linear ? genome satisfy this requirement: 493 bp, 784 bp, 2,396 bp, 2,409 bp, and 4,148 bp. A sixth clonable fragment of 9,866 bp is derived from the COS circle. Multiple copies of a single fragment or combinations of two different ? fragments can also be ligated into a single pBLU backbone. Three fragment plasmids can be constructed by combining the pBLU backbone with one of hte five fragments having one BamHI end and one HindIII end and with one of the eight fragments having two BamHI or two HindIII ends. Constructs with more than one unit of pBLU are also formed. Oly constructs with at least one unit of pBLU backbone contain an origin necessary for replication and the ampicillin-resistance gene required for growth on selective media.

1. Make scale drawings of all potential two-fragment (vector + insert) recombinant plasmids. Include the total bp size, fragment bp sizes, and the locations of BamHI sites and HindIII sites, the origin, the amplicillin-resistance gene, and the lacZ gene.

 

Reference no: EM1387491

Previous Q& A

  Find angular speed while on frictionless side of track

A car of mass = 800 travelling at 45.0 enters a banked turn covered with ice. The road is banked at an angle, and there is no friction between the road and the car's tires. What is the radius of the turn if = 20.0 (Assume the car continues in unif..

  Illustrate what is the estimate of the standard deviation

Illustrate what is the estimate of the standard deviation of the sampling distribution of sample proportions for whenever this process is under control?

  Explain why bni and carol do or do not have a contract

In the letter, BNI stated which Carol had ten days to accept. On May 5, Carol sent BNI a fax which stated, "The price for the audit seems too low. Would you consider paying $12,000? Explain why BNI and Carol do or do not, have a contract.

  Explain performance measures for balanced scorecard system

Explain three specific performance measures which could be utilized in Balanced Scorecard system in industry of your choice.

  Confidence interval for proportion of applicants that fail

Develop a 90 percent confidence interval for the proportion of applicants that fail the test. (Round your answers to 2 decimal places.)

  Show the conservation of mechanical energy

A 150 g ball and a 220 g ball are connected by a 36{rm cm}-long, mass less, rigid rod. The balls rotate about their center of mass at 130 rpm. What is the speed of the 150 g ball.

  Explain how much income must michael report due to the use

His ability to stay in the hotel without charge is based on the availability of empty rooms. Explain how much income must Michael report due to the use of the San Francisco hotel room?

  Genetics probability questions

Vitamin D resistant rickets is usually an X-linked dominant situation. The disease is caused by mutations the PHEX gene and results in bone deformity and bow leggedness.

  Corporatio rid of the variability in its lead-time per order

Suppose the corporation could pay C dollars per year to get rid of the variability in its lead-time per order. Illustrate what is the most it would be willing to pay to do this (and still have a 95% service level)?

  Determine the moons orbital speed

A motorcycle has a constant speed of 21.0 m/s as it passes over the top of a hill whose radius of curvature is 132 m. The mass of the motorcycle and driver is 343 kg. Determine the magnitude of the normal force that acts on the cycle.

Reviews

Write a Review

 

Similar Q& A

  Explanation for current potassium level

A patient with hypertension is being treated with hydrochlorothiazide and is due for a twenty-five mg dose shortly. Her latest potassium lab result is 2.4 mEq.

  Describe the cultural function of myths in general

They repeated the study with a larger number of samples but got the same results. What may be among their conclusions.

  What proportion of the f1 generation will be pink

When Mexican hairless dogs are crosses with normally haired dogs, about half the pups are hairless and half have hair. When, however, two Mexican hairless dogs are mated, about a third of the pups produced have hair, about two thirds are hairless, an..

  Effects of cholesterol on the people

Determine what can a disease like familial hypercholesterolemia tell us about the effects of cholesterol on the people without the disease?

  Which statement is inconsistent with the role of atp

Describe how a slight (but smooth) contraction differs from a vigorous contraction of the same muscle using the understandings of multiple motor unit summation.

  Find weight of the double stranded dna molecules

Assume you had supply of nucleotides made with atoms that were twice as heavy as normal, and you permitted some cells to replicate their dna once, using only heavy nucleotides.

  What force has to the student applied to the ice

The pH of the inner thylakoid space has been measured, as have the pH of the stroma and of the cytosol of a particular plant cell. Which, if any, relationship would you expect to find.

  Question about recombination frequency

CC DD and cc dd individuals were crossed and hte F1 generation was backcrossed to get cc dd parent. 903 Cc Dd, 897 cc dd, 98 Cc dd. c and d are 10 m.u apart.

  Proteins that exist on earth

Discuss what you would require to know to answer the following question: of all proteins that exist on earth at this time, what fraction of them have a known structure?

  Design a biotechnological approach for the amino acid

Design a biotechnological approach to getting better the amino acid content of wheat. How are you going to communicate your decision to the surgeons, medical center employees, governing board, and to public.

  What would be trc for a male with the genotype

What would be TRC for a male with the genotype AABbCcDD? For a female. In contrast, each dominant allele in an individual's genotype contributes 12 ridges to the TRC in males and 10 ridges to the TRC in females.

  Why did child become so upset

A young mother is encouraging her son to try another bite of a disliked food. The child is quietly whining, but when the mother spreads the food around to cool it, the child becomes hysterical. Why did child become so upset? Recognize the cognitive s..

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd