You now want to express the gene in E coli, but unfortunately it has an intron in it that will not be processed out in bacteria, hence it must be removed.

(a)  Design PCR primers (indicate regions of primers with black, blue and red colours by sketching on the plasmid map) for a PCR approach that could result in the deletion of the intron from this gene so that it can be expressed in the resulting plasmid in E. coli.

(b)  Sketch what the two PCR amplified strands would look like from the parental plasmid,

Sketch what the subsequent two PCR amplified strands would look like from newly synthesised DNA [DNA strands indicated in (b)].