What is fluorescent microscope, Biology

Assignment Help:

What is Fluorescent Microscope?

In above said microscopes, image is produced from light that passes through a specimen. In fluorescent microscope, however, specimen image is formed because of the light emitted by the object itself. This microscope is used more frequently to visualize specimens that are tagged with a fluorescent dye. The source of illumination here is an ultraviolet light obtained from a high-pressure mercury vapour arc lamp or hydrogen quartz lamp.

Fluorescent dye absorbs the ultraviolet light of desired wavelength and re-emits the energy in visible range. Fluorescent portion of the dye becomes visible against a black background. The fluorescent microscope has become an essential tool in medical microbiology and microbial ecology for identifying the pathogens and other microbes. The forms of light microscopy just considered, forms two-dimensional images. Besides, certain new forms of light microscopy have been developed which gives three-dimensional images. These are differential interference contrast microscopy, atomic force microscopy and confocal scanning laser microscopy.


Related Discussions:- What is fluorescent microscope

What are the properties of titanium, What are the Properties of titanium ...

What are the Properties of titanium Titanium has several favourable properties like low specific gravity with a density of 4.5 gm/cm 3 , high heat resistance and high strength

What microbiology lab technician left the safranin out, If a microbiology l...

If a microbiology lab technician left the safranin out of the Gram stain procedure, what would be the result?

Class turbellaria, plz answer me how I arrange my topic with its order ?

plz answer me how I arrange my topic with its order ?

Artificial respiration, Artificial respiration First - aid for drown...

Artificial respiration First - aid for drowning victims should aim to restore respiration as quickly as possible. First of all, nose, mouth and throat of the victim shoul

Ganong''s potometer, What happens to the movement of the air bubble if the ...

What happens to the movement of the air bubble if the apparatus is kept in i. In the dark ii. In sunlight iii.In front of a fan

Determine the molecular function, Please answer the following three questio...

Please answer the following three questions on Sequence Z: Metadata The GO Ontology is a very widely-used resource in the bioinformatics community as a tool to annotate

Plant Taxonomy, Ecology and genetics as taxonomic units

Ecology and genetics as taxonomic units

Iron age, Explain the factors that led to the decline of Science in Europe ...

Explain the factors that led to the decline of Science in Europe during iron age

Describe about psycho-social change in children, Describe about Psycho-soci...

Describe about Psycho-social Change in children? Adolescence is a period of maturation for both mind and body. Along with the physic4 growth, emotional and intellectual develop

Define drug effects on metabolism of dietary components, Define Drug effect...

Define Drug effects on Metabolism of dietary components? Drugs can also compete with, or inhibit, the metabolic conversion of some micronutrients to their active metabolites,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd