Use pcr to sequence the particular gene, Biology

Assignment Help:

You wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volume. How would you dilute your primer, which is currently at 1mg/ml to provide 3.2pmole in 2 micro liters if the molecular weight of your primer is 13,425?


Related Discussions:- Use pcr to sequence the particular gene

Dna, What is a helix?

What is a helix?

Determine what is dermal branchiae, Determine what is dermal branchiae? ...

Determine what is dermal branchiae? External extensions of outer epidermis and peritoneum of the echinoderm body cavity. Both outer epidermis and inner peritoneum are lined wit

Osteoporasis, OSTEOPORASIS - Reduction in bone tissue mass causing weak...

OSTEOPORASIS - Reduction in bone tissue mass causing weakness of skeletal strength. It leads to crush fracture. It occurs in pastmenopausal women & elderly man. Common

Explain starch phosphates monoesters, Explain Starch phosphates monoesters ...

Explain Starch phosphates monoesters and  Hydroxypropyl starches   Starch phosphates monoesters Emulsifying agent and thickening agent. Hydroxypropyl starches

LPN, In many tests it is acceptable to read a positive result before the in...

In many tests it is acceptable to read a positive result before the incubation time is completed. why is this not the case with starch agar?

Vertebrate heart, VERTEBR A T E HEART Heart = Cardia. Most import...

VERTEBR A T E HEART Heart = Cardia. Most important organ of body. Main function is to pump the blood. Study of heart is cardiology. In heart auricles and ventricles pr

Light harvesting in green plants, Sunlight is absorbed by chlorophyll molec...

Sunlight is absorbed by chlorophyll molecules.  Chlorophyll is a porphyrin in that nitrogen atoms are coordinated to a magnesium ion instance for it is a magnesium porphyrin.  This

Symptoms of oesophagitis, Q. Symptoms of oesophagitis? The symptoms of ...

Q. Symptoms of oesophagitis? The symptoms of oesophagitis include heartburn or pyrosis, iron-deficiency anaemia due to chronic tissue bleeding, aspiration, which may cause coug

Cryo preserved allografts -surgical techniques, Cry o Preserved Allograf...

Cry o Preserved Allografts (Homograft) :  As these are not mounted, a free hand suturing in two layers has to be done. The valve is thawed by protocol. The septal muscle i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd