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Assume an experimental target that is to make vast amounts of a particular DNA fragment in pure form from a combination of DNA fragments. Whereas the DNA fragments can be introduced into bacterial cells the most or all will lack the ability for self-replication and will fast be lost. Furthermore, two kinds of DNA molecule are known that can replicate autonomously in bacterial cells; plasmids and bacteriophages. Plasmids are little circular double-stranded DNA molecules which exist free inside bacterial cells, frequently carry certain genes which confer drug resistance and are self-replicating of the genes. If a recombinant DNA molecule is establish by joining a foreign DNA fragment to plasmid or bacteriophage DNA then the foreign DNA is replicated when the plasmid or phage DNA is replicated. In that role the plasmid or phage DNA is known as a vector. Todays, a population of recombinant DNA molecules can be build, every recombinant molecule having one of the foreign DNA fragments in the original combination.
Q. What is Insulin Dependent Diabetes Mellitus? Mostly children and adolescents suffer from this type of diabetes however; it may appear in adults and elderly too. In this, the
What is Isonietric Exercise in dynamic auscultation ? Can be carried out by doing handgrip exercise which is sustained over 20-30 second. It results in transient increase in SV
What are plasmids? Plasmids are circular DNA molecules there in the genetic material of some bacteria. They might be containing genes responsible for bacterial resistance to so
Caruncle - Seed Appendages This is a white, collar-like structure borne on the micropylar end of the seed in many members of the Euphorbiaceae, such as castor, Ricinus communi
Define the Dietary Requirements of phosphorus? We read in the previous section that the requirements of phosphorus are closely linked with those of calcium. The phosphorus req
where does kreb cycle takes place
Define the Disturbances in Fluid Balance? The correct functioning of cells and tissues depends on appropriate concentrations of nutrients; so any abnormal loss or accumulation
Reproduction and Life Cycles – Protozoan Asexual reproduction occurs in all protozoan through fission, budding and cyst formation. In this method the organism reproduces to fo
Initial Maintenance Steps of implants It is very essential, that from the beginning of the patient interaction regarding the implant therapy, through the diagnosis and planning
The Nurse's Role in Relieving the Child's Stres s Turning passive experiences into active ones that facilitate the child's healthy - Problems-I adjustment to hospitalization
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