The principle of dna cloning, Biology

Assignment Help:

Assume an experimental target that is to make vast amounts of a particular DNA fragment in pure form from a combination of DNA fragments.  Whereas the DNA fragments can be introduced into bacterial cells the most or all will lack the ability for self-replication and will fast be lost. Furthermore, two kinds of DNA molecule are known that can replicate autonomously in bacterial cells; plasmids and bacteriophages. Plasmids are little circular double-stranded DNA molecules which exist free inside bacterial cells, frequently carry certain genes which confer drug resistance and are self-replicating of the genes.  If a recombinant DNA molecule is establish by joining a foreign DNA fragment to plasmid or bacteriophage DNA then the foreign DNA is replicated when the plasmid or phage DNA is replicated.  In that role the plasmid or phage DNA is known as a vector.  Todays, a population of recombinant DNA molecules can be build, every recombinant molecule having one of the foreign DNA fragments in the original combination.

 


Related Discussions:- The principle of dna cloning

Explain about the microcolony deft, Explain about the Microcolony DEFT? ...

Explain about the Microcolony DEFT? It allows determination of viable cells. Food homogenate is filtered through a DEFT membrane and then placed on the surface of appropriate c

Agro industrial-early embryonic mortality, Early embryonic mortality E...

Early embryonic mortality Embryo mortality is a serious problem for farmers, practitioners and researchers. The establishment and maintenance of pregnancy is a highly complica

Digestion, explain digestion in protozoans

explain digestion in protozoans

Are nematodes diploblastic or triploblastic animals, Are nematodes diplobla...

Are nematodes diploblastic or triploblastic animals? Just like platyhelminthes, nematodes are triploblastics, i.e., they show three germ layers (ectoderm, mesoderm and endoder

Indications for surgery, Indications for Surgery :  Patients with moderate...

Indications for Surgery :  Patients with moderate or severe tricuspid regurgitation that is functional will need tricuspid annuloplasty at the time of correction of mitral valve d

Define the term parasite -giardia, Normal 0 false false fal...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Define the term - the dna transistor, Regarding the article "The DNA Transi...

Regarding the article "The DNA Transistor" which of the following is a false statement? A. The DNA transistor refers to the use of an electrical sensor to read one nucleotide a

Spoilage of fish, Q. Spoilage of Fish and other Sea Foods Fish and other...

Q. Spoilage of Fish and other Sea Foods Fish and other marine foods are second only to meat and poultry as a staple animal food around the world. India is one of the important f

Describe the mechanism responsible for the cessation, Describe a. the facto...

Describe a. the factors responsible for promoting lactation, and b. the mechanism responsible for the cessation of lactation when a mother weans her baby.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd