Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Enzymes and recombinant DNA technology
Recombinant DNA technology, also called as genetic engineering makes use of a variety of enzymes, particularly restriction endonucleases (from bacteria) and DNA ligases to insert extra genes into cells with the help of vehicles termed 'vectors'. One important group of vectors is 'plasmids', which are small, circular, cytoplasmic molecules of DNA, acting as extrachromosomal genes in bacteria. It is possible to extract and purify plasmids and to insert extra genes into the circles. These altered plasmids can then be taken up again from the medium by the microorganism. Then as the bacterium is grown in culture, the inserted gene will be replicated together with the vector. The production of identical copies is termed as 'cloning.
Another group of vectors are the variants of a bacterial virus known as λ phage. The phage DNA is about 45 kb long, of which the middle third has no role in the infection process and can be replaced by another piece of DNA of about the same length (again by means of restriction endonuclease and ligase enzymes) without affecting the ability of the phage to infect bacteria and be reproduced in abundance. Genes or gene fragments of about 15 kb length can be inserted and cloned in this way, in contrast to the limit of 10 kb when plasmids are used as vectors.
Q. What are the mechanisms of hemorrhage contention called? The physiological mechanisms of hemorrhage contention one of them is blood clotting are generically named hemostatic
economic importance of tubularia
In which meiotic division does the separation of identical chromatids occur? After the end of this process what are the ploidies of the new cells? The separation of identical c
DEXTRIN Intermediate product of hydrolysis of starch. It consists of maltose & glucose. It is found in yeast & some bacteria.
Production of haploids by tissue culture,
Stereochemistry of Amino Acids: Optical Properties All amino acids (except glycine) rotate the plane of polarized light because of the presence of an asymmetric center at C-2
Q. How does phosphocreatine act in the muscle relaxation and contraction? Phosphocreatine is the major means of energy storage of the muscle cells. During relaxed periods AT
Threatened Species - Wildlife Many plant and animal species are threatened by the possibility of extinction. However, the seriousness of the threat varies. For example, a spec
Define Hormonal imbalance as a factor for obesity? Certain diseases associated with secretion of hormones, e.g., hypothyroidism, hypogonadism and Cushing's syndrome exhibit obe
B a b e s i o s i s It is also known as red water disease or cattle tick fever and is characterized by fever, intravascular haemolysis, acute anaemia and haemoglobinur
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd