Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment.....
Question:Suppose restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment. NarI cuts DNA also into two fragments; fragments 1200 and 4800 bp long. Relative to HindIII cut site, there are two probable ways in which NarIcould have cut DNA. How can you determine correct cleavage site for NarI with respect to HindIII?
Answer
To determine this, one should subject DNA to a double digest in which both enzymes are allowed to cut DNA at the same time. When double digest is allowed to take place, if three fragments which appear upon electrophoresis of the restricted DNAare 400, 800 and 4800 bp, then the only possible way in which data can be interpreted is with 1200 bp NarI fragment containing the HindIII recognition site 800 bp from the end of linear piece of DNA. If 4800 bp NarI fragment contained the cut site, you would visualize fragments of sizes 800, 1200, and 4000 bp after electrophoresing the doubly digested DNA.
How do the availability of water and light and the climate affect the growth of a population? The availability of water and light and the climate are abiotic factors that limi
Patent Ductus Arteriosus (PDA) PDA is one of the most common cardiac anomalies. It occurs twice as frequently in girls as in boys. In patent ductus Arteriosus there is
Shrub Stage - Xerarch Sufficient soil is formed in the herbs stage, for supporting the woody plants or the shrubs. They migrate with the help of seeds or rhizomes from the adj
Explain Lipoprotein (Lp(a) Excess ? While cholesterol and triglycerides are important lipid risk factors, another lipoprotein factor called Lipoprotein (a) is thought to play a
Levels of organization of Matter- Metazoa The smallest structural units of all matter are subatomic particles, mainly electrons, protons and neutrons. The next larger units a
Q. Which are the major positive ions found in living beings? The major cations found in living beings are the calcium cation (Ca++), the potassium cation (K+), the sodium catio
Explain the term Brown-Sequard Syndrome Brown-Sequard syndrome is a rare spinal disorder that results from an injury to one side of the spinal cord in which the spinal cord is
Pisciculture: This is the culturing of fishes. There is a principal form of aquaculture called Fish farming, while other methods can come under mariculture. Fish farming main aim i
Particulates: These are suspended droplets, solid particles or mixture of two. So all the atmospheric substances that are not gases are called particulates. A number of term
Define Principle for Determination of Blood Glucose by Nelson Somogyi Method? Proteins are precipitated from the sample and protein free filtrate is heated with alkaline copper
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd