Prior preparation for fixing and staining animal tissue and/or plant tissues: Find out what procedure the lab technician follows for preparing fixative (either plant andor animal), alcohol series and stain for animal (acetoorcein or aceto carmine) andor plant (safianin).

Enquire what calculation the technicians undertake to prepare the various solutions and stains so that they are neither in excess nor too little. Also whether the quantities are prepared on the basis of the requirement of each student or are some other calculation used?

Also whether the technicians prepare all the solutions a day or two before the experiment or on the same day. After the experiment do the technicians retain any of the prepared chemicals or do they discard them? If the chemicals are not discarded, how long can they be-kept without deterioration?