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Define Principle of colorimetric method-2, 4 dinitrophenylhydrazine?
Ascorbic acid is oxidized to dehydro ascorbic acid by copper (Cu++) ions. 2, 4 dinitrophenyl hydrazine reacts with the ketone group of dehydro ascorbic acid under acidic conditions to give osazone. The depth of the yellowish orange coloured osazone produced upon acidification with sulphuric acid is directly proportional to the amount of ascorbic acid present and can be measured photometrically at 540 nm wavelength.
As you carry out the procedure in the laboratory you will understand the method even better. So then let's get started and practically learn the two methods we have studied above. There are four activities in this practical.
Q. Protein Requirement during congestive cardiac failure? Protein: The protein requirements remain the &me as healthy adult men and women, About 0.8 - lg of protein per kg
Explain the Minimum Requirement of nutritional needs? Minimum nutrient requirement is defined as the 'lowest amount of the nutrient ji-om the diet that will prevent clinically
STRUCTUR E OF A MYOFIBRIL - The dark bands of the myofibril are termed the A-bands (Anisotropic bands). Each A-band has at its middle a light zone called H-zone (Henson'
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Define about Iodine Metabolism? Now, we will very briefly study how iodine is absorbed, distributed in the body and ' excreted out. Like other nutrients, dietary iodide is eith
Classification of Viruses No evolutionary or phylogenetic exist between viruses. A nature system of classification cannot, therefore, be devised for viruses. Holmes (1948)
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