Nuclear transfer method for gene transfer, Biology

Assignment Help:

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human diseases. The production of Dolly followed by subsequent confirmation in cow and mice successfully established a cloning technology, which have opened the new horizons of application of nuclear transfer technique in production of transgenic animals. Current available technique of pronuclear microinjection of a DNA expression cassette is inefficient and expression levels are unpredictable. Nuclear transfer (cloning) offers an appealing alternative. Hence, one could perform gene targeting in cell lines that is able to donate its nuclei to recipient oocytes. This would allow selection of the most productive cell line showing high expression for conversion into highly productive animals, and all animals will be transgenic. For example, the time from microinjection to a milk ­producing flock is 3.5 years, if the founder is female, and 2.5 years, if it is male. With somatic cloning this time can be reduced to 1.5 years. This saving of time will be greater with cow. Production of transgenic founder animals by nuclear transfer also uses less than half the number of experimental animals than a pronuclear injection.


Related Discussions:- Nuclear transfer method for gene transfer

Explain night blindness - micronutrient deficiencies, Explain Night Blindne...

Explain Night Blindness - micronutrient deficiencies? Night blindness is the earliest symptom of Vitamin 'A' deficiency. The reduction in the supply of vitamin A aldehyde i.e.

Formulate a specific hypothesis, A professor wonders whether good study hab...

A professor wonders whether good study habits lead to better grades in the final exam. Formulate a specific hypothesis and then design an experiment to test it. In your answer outl

Chest and leg wound complications, Chest and Leg Wound Complications ...

Chest and Leg Wound Complications :  The patients under going CABG are usually elderly, obese and nearly a quater of them are diabetic. So sonic of them get superficial or d

Aves, detail information about ave?

detail information about ave?

How do the climate affect the growth of population, Q. How do the availabil...

Q. How do the availability of water and light and the climate affect the growth of a population? The availability of light and water and the climate are abiotic factors that li

List a few applications of starches in the food industry, List a few applic...

List a few applications of starches in the food industry. A few applications of starches in the foods industry include thickener, a fat sparing agent, adhesive, binder,

Death of post-thymic lymphocytes induced by corticosteroid, A 21-year-old w...

A 21-year-old woman presents with a 3-month history of malaise, joint pain, weight loss, and sporadic fever. Her temperature is 38°C (101°F). The serum antinuclear antibody (ANA) t

Anatomy and physiology, write two part each in the body where cuboidal epit...

write two part each in the body where cuboidal epithelia tissue are found in absorption and excretion

Explain the wash technique used in sterilization process, Q. Explain the Wa...

Q. Explain the Wash Technique used in sterilization process? Use of artificial nails is contraindicated as is use of colored nail polish. Nails should be short enough to allow

Decreased performance of the removable prosthesis, Removable soft tissue-bo...

Removable soft tissue-borne partial dentures have one of the lowest patient acceptance rates in dentistry. Those patients wearing the partial dentures often exhibit greater mobilit

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd