Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Non-viral vectors
Viral vectors are highly efficient but when it comes to large scale production at the commercial level, non-viral serve as a better choice. These methods produce low immunogenic response and hence are also considered to be safe. With the recent advancement in this area the efficiency of transfection has also improved (Anderson, N.D). The best examples of non viral vectors include:
Apart from these few other non viral methods include, the use of DNA complexing reagents, liposomes etc.
Table 1: A table showing comparison of vectors (Gould and Favorov, 2003, Table 1)
Vector
Transgene capacity
Immunogenicity
Genome integration
Long-term expression
Transfer into dividing (D) and quiesent (Q) cells
Plasmid-naked
Unlimited
Low
No
Only in muscle
D and Q
Plasmid-complexed
Ad 1st generation
5 kb
High
Ad 2nd generation
8 kb
Ad gutless
37 kb
Less
Longer
AAV
4 kb
Yes and episomal
Yes
HSV
35 kb
Retrovirus
Up to 8 kb
D
Lentivirus
What is the main moral problem about the cloning of human individuals? Besides biological perils, a very serious moral problem includes the nucleus transplantation technology c
can you help me with my essay
Q. What is the destination of the notochord in protochordates and in vertebrates? In vertebrates the notochord disappears and gives birth to the spinal column known as vertebra
1. Hazard Is An Extreme Event Whereas Disaster Is Its Consequence. 2. A hazard can occur without human interference but it may become a disaster on account of careless
adaptation and distribution of synaptura (flat fish)
What are hydrophobic molecules (or hydrophobic molecular regions)? What are hydrophilic molecules? How can they be characterized in relation to their polarity? Hydrophobic mol
Q. What is the vena cava? Which type of blood circulates within the vena cava? The vena cava is either of two large veins that debouch into the right atrium the superior vena c
what is the mode of nutrition in cow
What is Physical Accommodation Physical Accommodation is the actual physical deformation in the lens power. It is measured in diopters, e.g., if the focussing power of the eye
In the eukaryotes, eukaryotic release factor eRF-1 recognizes all three termination codons that are UGA UAA, and UAG and, with the help of protein eRF-3, terminates translation.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd