Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Non-viral vectors
Viral vectors are highly efficient but when it comes to large scale production at the commercial level, non-viral serve as a better choice. These methods produce low immunogenic response and hence are also considered to be safe. With the recent advancement in this area the efficiency of transfection has also improved (Anderson, N.D). The best examples of non viral vectors include:
Apart from these few other non viral methods include, the use of DNA complexing reagents, liposomes etc.
Table 1: A table showing comparison of vectors (Gould and Favorov, 2003, Table 1)
Vector
Transgene capacity
Immunogenicity
Genome integration
Long-term expression
Transfer into dividing (D) and quiesent (Q) cells
Plasmid-naked
Unlimited
Low
No
Only in muscle
D and Q
Plasmid-complexed
Ad 1st generation
5 kb
High
Ad 2nd generation
8 kb
Ad gutless
37 kb
Less
Longer
AAV
4 kb
Yes and episomal
Yes
HSV
35 kb
Retrovirus
Up to 8 kb
D
Lentivirus
Strategies for genetic characterization and conservation - During last 20 years concerted efforts have been made to create awareness among all the developmental organizations in th
What are the main degenerative diseases of the heart? The major degenerative diseases of the heart are heart failure, arrhythmias, valvular heart diseases, coronary insufficie
GREGOR JOHANN MENDEL 1. Mendel was born on 22 July 1822 at Heizendorf in Austria at Selesia village. 2. Mendel was worked in Augustion Monastry as monk at Br
Define Use of Isotopically Labelled Nutrients? Nutrient Turnover Radioactive labelled nutrients are used to know the total body pool and the compartment in which it is stored.
List of Conditions Requiring Rapid Treatment of Hypertension 1) Cardiac: • Acute aortic dissection • Acute left ventricular failure • Acute or evolving myocardial i
HISTORY OF THE CELL Term "Cytology" was given by Hertwig , he also wrote a book on " Cell and Tissue ". Father of cytology = Robert Hooke. Father of Modern cytology
Phosphorus Phosphorus has been known as “master mineral” because it is involved in most metabolic processes. Phosphorus, which is stored in bones and teeth, is often discussed
What are the result of Arterial Switch operation ? The operation is highly specialised. When carried out in experienced centers the mortality for simple TGA and TGA with VSD i
Q.Explain about Stress Testing in Women? Data on mortality in men is about 2.5 times that of women. At younger ages, however, CAD in men exceeds that found in women by 5 to 1.
how does a cell work ?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd