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Q. Mismatch and Recombinational repair?
Mismatch repair:
1. Mismatches can take place when DNA polymerase inserts wrong nucleotide during replication 2. Mismatch repair is"coupled" to replication
Recombinational repair:
1. Takes place during DNA replication 2. Major steps: (a) DNA polymerase skips over damaged DNA leaving a gap opposite the lesion (b) Undamaged parental strand recombines into the gap (this is facilitated by recA protein in E. coli) (c) the new gap in parental strand is filled by DNA polymerase and ligase.
Explain about the Vacuum Evaporators? It is common to construct various vacuumised vessels in series that is why the product moves from one vacuum chamber to the next and thus
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