Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
1. Northern blotting
The quantity of rna species can be quantified by calculating the quantity of rna through northern blotting which provide size and sequence information regarding the rna molecule.
In this technique a sample of rna is separated by an agarose gel and hybridise to a radioactive rna probe that is corresponding to target sequencer. The radio labeled rna is then identified through an autoradiograph.
2. Reverse transcriptase-real time quantitative pcr
The other technique for quantification of mrna is rt-qpcr, in this technique reverse transcription creates a dna template from the mrna, this single stranded template is known as cdna. The c dna template is amplified through rt-qpcr in a quantitative step during which fluorescence emitted through labeled hybridization probes or intercalating dyes variates as dna amplification process proceeds. With the standard curve qpcr can create an absolute measurement of the number of copies of real rna.
3. Hybridisation micro array
This method comprises of single array or chip that include probes to know transcript level for every known gene in the genome therefore assist in quantification of rna species.
4. Serial analysis of gene expression and rna sequencing are tag based methods which can give a relative measurement of the cellular concentration of diverse rna.
Biota of Lakes Lakes exhibit life zones. So far you have been reading about the vertical zonation of lakes. The lakes can be divided into horizontal zones as well. This divis
State Diploblastic in brief. Organisms formed from only two primitive cell layers- ectoderm and endoderm. Though there may be some type of a matrix between two cell layers, gen
how to write a project on phylum arthropoda
Q. What is the spinal cord? Of which elements is the spinal cord constituted? The spinal cord is the dorsal neural cord of vertebrates it is the part of the central nervous sys
Zone of Polarizing Activity (ZPA) The A-P axis is the 1 st axis to be fixed in the presumptive limb mesoderm at a very early stage. On the basis of results of a series of cur
DRU G NOMENCLATURE - Generally drug has three types of names - Chemical, Proprietary & Non-proprietary. Chemical Name - Indicates chemical nature. Proprietary Name -
How many pints (473 ml) of Fat Tire amber ale can an 80 kg person drink in Arizona before reaching the blood alcohol limit? Fat Tire's ethanol concentration of 5.3% (v/v). Assume c
Q. Principles of Binomial Nomenclature? There are certain, basic principles of binomial nomenclature which are as follows: i) Different nomenclatural systems are independent
What is reproduction?
Degradation of Ecosystem Ancient man was simple-minded food gatherer and hunter. He looked upon nature with awe and respect and in fact he worshipped it. But from the time he
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd