Methods including quantification of rna species, Biology

Assignment Help:

1.   Northern blotting

The quantity of rna species can be quantified by calculating the quantity of rna through northern blotting which provide size and sequence information regarding the rna molecule.

In this technique a sample of rna is separated by an agarose gel and hybridise to a radioactive rna probe that is corresponding to target sequencer. The radio labeled rna is then identified through an autoradiograph.

2.    Reverse transcriptase-real time quantitative pcr

The other technique for quantification of mrna is rt-qpcr, in this technique reverse transcription creates a dna template from the mrna, this single stranded template is known as cdna. The c dna template is amplified through rt-qpcr in a quantitative step during which fluorescence emitted through labeled hybridization probes or intercalating dyes variates as dna amplification process proceeds. With the standard curve qpcr can create an absolute measurement of the number of copies of real rna.

3.   Hybridisation micro array

This method comprises of single array or chip that include probes to know transcript level for every known gene in the genome therefore assist in quantification of rna species.

4.  Serial analysis of gene expression and rna sequencing are tag based methods which can give a relative measurement of the cellular concentration of diverse rna.

 


Related Discussions:- Methods including quantification of rna species

Classification of diabetes mellitus, Classification of Diabetes Mellitus ...

Classification of Diabetes Mellitus a) Type1Diabetes Mellitus i) Type 1 a ii) Type 1 b b) Type 2 Diabetes Mellitus c) Others i) Gestational Diabetes Mellitus

How many possible codons are there, A codon comprise three nucleotides. The...

A codon comprise three nucleotides. There are four possible nucleotides, each of which has an equal probability of being in any one of three spots. How many possible codons exist?

Can you explain about cardiomyopathies, Q. Can you explain about Cardiomyop...

Q. Can you explain about Cardiomyopathies? Cardiomyopathy is a primary disorder of heart muscle that may cause cardiac dysfunction and is not related to any obvious disease pr

Ecological isolation, Ecological isolation is based on the fact that popula...

Ecological isolation is based on the fact that population shows preference to one habitat over the other. This extensive forests become barriers to the dispersal of organims living

Explain the culture media and its types, Explain the Culture Media and Its ...

Explain the Culture Media and Its Types? A culture medium (Pl. media), we already know, is a solid or liquid preparation containing all the nutrients required by microorganisms

Middle ear, MIDDL E EAR - Protected by tympanic bone. It's cavity...

MIDDL E EAR - Protected by tympanic bone. It's cavity is tympanic cavity. It is lined by simple cilliated columnar epithelium. It is connected to naso pharynx by Eu

Water supply, A rural town in an established agricultural area with no pros...

A rural town in an established agricultural area with no prospects for extensive development is located along a river that is used as the source of water supply for the community.

Define drawbacks for underwater weighing method, Define drawbacks for under...

Define drawbacks for underwater weighing method? Since the major drawbacks with UWW method are that the subject needs to be totally submerged underwater and exhale all of the a

Explain development of excretory system, Explain Development of excretory s...

Explain Development of excretory system? The filtration rate of kidney is low and infants find it difficult to eliminate high concentration of solutes. However, by the end of t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd