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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Protein denaturizing can be source by temperature variation, pH change, and changes in the concentration of surrounding solutes and by other processes. Most proteins are denatured
Explain Crossing Over in genetics? Crossing over is when the arms of homologous chromosomes exchange segments (and therefore genes) with each other. Recall that one of the homo
list and explain the modes of nutrition
Q. Why is it said that during photosynthesis carbon dioxide is improved to form glucose? During photosynthesis carbon dioxide is energetically improve with hydrogen from water.
Q. What structure of the eye produces tears? Lachrymal gland, which is a part of larger lachrymal apparatus, produces tears that flow over anterior surface of the eye. Most of
Given how the Na+/K+ pump works, which part of the cell (inside or outside) will have an excess of negative charge?
Q What is the constitution of the endocrine system? The endocrine system is constituted by the hormones and the endocrine glands they secrete. Q. What is the histological n
#question.why obelia is considered of special interest in zoology.
What is Primary Growth in Shoot? The growth in height of a plant stem results from the division of cells at the apex, or tip of the stem, called the shoot tip. Here, a group o
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