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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Inorganic Substances - Abiotic Components There are about forty elements that are required in various processes of living organisms. Some of these are macronutrients which the
trycocyst
Cells are classified into eukaryotic or prokaryotic. Prokaryotic cell is that with no a delimited nucleus. Eukaryotic cells are those with nucleus delimited by membrane.
Define Iodothyronine Deiodinases - functions of selenium in humans? Another group of selenoproteins are the iodothyronine deiodinases essential for the conversion of thyroxine
Treatment of travelers'diarrhea The most common cause of travelers' diarrhea, usually a self-limited illness lasting several days, is infection with noninvasive enterotoxigeni
Q. Dietary tips for hyperlipideinic patients? It is important to note here that the dietary modifications need to be individual in each patient and these should be tailored lo
Inhibition Model - Models of Succession According to this model, succession is very heterogenous because the development at any one site depends on who gets there first. Speci
Debate over ethics of using gene therapy for treating human beings started since the discovery of recombinant DNA technology. The mere fact that it involves the alteration of DNA w
Explain in brief Chitinous? A structure which is composed of chitin, a complex carbohydrate composed of linearly arranged Nacetyl-glucosamine units. Chitin is a characteristic
What was the Oral Status of the Patient It is imperative that the patient carry out a strict oral hygiene regimen as dental plaque is one of the main factors that leads to impl
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