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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Features of Plant and Animal Cell However plant and animal cells also differ. One of the most prominent features of the plant cell is its huge vacuoles filled with cell sap.
Why can the amnion also be considered an adaptation to terrestrial life? The amnion is also an adaptation to dry land since one of its functions is to prevent desiccation of th
What is eutrophication" describe its consequences on the life of plants and animals living in such waters. Why is oxygen depletion in a eutrophicated water-body faster at nigh
Q. Does the mitosis properly occur after or before the interphase? Is it a simple "point of view" issue? Mitosis have to be considered a succeeding phase after interphase since
Explain Kashin Beck disease caused by selenium deficiency? Kashin Beck disease was identified to affect growing children in parts of Siberian Russia and China. It is characteri
Q What is the usual reproduction cycle of a DNA virus? A usual virus has proteins on its capsid that bind to the outer membrane of the host cell. In the place where the virus a
Define the Importance of Zinc in Humans? Zinc deficiency in humans was reported by A.S. Prasad among people consuming mostly breads and very little animal protein in Middle Eas
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What is the criterion used to classify hosts as intermediate hosts or as definitive hosts? The criterion used to classify hosts as intermediate hosts or as definitive hosts is
Define about Calcium and Osteoporosis? Gain in bone mass occurs throughout childhood; however, during adolescent growth spurt, the gain in bone mass, as well as, calcium retent
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