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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
You need to prepare 500 ml of a solution containing 10 mM Tris, 0.15 M NaCl and 1 mg/ml SDS. At your work disposal are stock solutions containing 1 M Tris, 2.5M NaCl and 10% (w/v)
High fibre diets High fibre diets: The patients are advised to eat high fibre cereals as whole grain flour and bread, whole grain bread cereals, whole wheat pasta and b
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what is Acute Massive Pulmonary Embolism ? Acute Massive Pulmonary Embolism : Before the era of open-heart surgery, Trendelenburg (1908) described technique of open p
TYPES OF MENTAL DISORDER - 1 . Psychosis 2 . Neurosis 3. Personality & character disorders 1 . PSYCHOSIS - It effects mental capa
Explain Iron Uptake by Cells? Iron participates in a large number of biochemical reactions. However, for iron to perform any function, it first needs to be taken up by the cell
Explain the Gomez classification? It will be of interest to you to learn that in Mexico, a child specialist named Gomez and his co-workers proposed a classification expressing
MIDDLE LAMELLA It is thin, amorphous, intercellular matrix between 2 adjacent plant cell. The cells of plant tissue generally cemented together by an intercellular matrix
Feeding mechanism in holozoic animal
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