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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Define Precautions for preparation of bacterial smear 1. The slide should not be greasy. 2. Hold the slide by their edges only. 3. Label should not come in contact with s
What is the type of genetic inheritance of daltonism? Is daltonism more frequent in men or in women? What is the physiological explanation for the daltonism ? The Daltonism
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What is Gene therapy Gene therapy targets non germ line cells, ie somatic cells, so will not directly affect gene pool. The frequency of the allele for the genetic disease w
Where do the two neuronal pathways diverge For both the neuronal pathways i.e. light reflex and near reflex the efferent fibres go to are 3rd cranial nerve and then to sphinct
tell me the general characters of monopisthocotylea
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Planning of Nursing Care Planning of the nursing care depends on the decision of the surgery. The surgery may be performed in two stages depending upon the degree of the d
Q. What is severity of the oesophagitis? The severity of the oesophagitis resulting from oesophageal reflux is determined by the content of gastric reflux mucosal resistance,
why is the phylum annelida in trouble?
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