Isolate genomic dna, Biology

Assignment Help:

You may have heard in the news currently that a new species of frog was discovered within a rainforest.

You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.

Questions:

a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.

b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.


Related Discussions:- Isolate genomic dna

Haptotaxis - modes of cell movement, Haptotaxis - Modes of Cell Movement ...

Haptotaxis - Modes of Cell Movement Haptotaxis considers to the directed movement of cells in response to a concentration gradient of an adhesive molecule that might be presen

Digestion in the intestine, Digestion in the Intestine About  2  to  3 ...

Digestion in the Intestine About  2  to  3  liters  of  alkaline  fluid  is secreted every  day  by  the intestine  and completes the digestive process.  Sodium bicarbonate and

Explain the acquired deficiency of the vitamin k metabolism, Why are some t...

Why are some types of hemorrhagic diseases caused by genetic or acquired deficiency of the vitamin K metabolism? Lack of vitamin K predisposes to hemorrhages as this vitamin i

Ageing, AGEING - The appearance of some changes as the body grows older...

AGEING - The appearance of some changes as the body grows older is called ageing. It ultinately leads to death. It can be defined as deteoration in structure and function of

Define factors that affecting the calcium absorption, Define Factors that A...

Define Factors that Affecting the Calcium Absorption? It is a well known fact that the amount of calcium that we eat need not be the amount of calcium that gets absorbed. The d

Affect the rate of cellular respiration, how does temperature affect the ra...

how does temperature affect the rate of cellular respiration? please explain with great detail!

Show the directly visible chemical characters, Q. Show the Directly Visible...

Q. Show the Directly Visible Chemical Characters? Very few chemical substances in plants can be observed directly, but the few substances such, starch grains which are pres

Developmental concepts unique to infants and young children, Developmental ...

Developmental Concepts Unique to Infants and Young Children The cognitive and social emotional development of infants and very young children has unique features. This age rang

Show major treatments of diabetes mellitus, Q. What are the major treatment...

Q. What are the major treatments of diabetes mellitus? The general goal of the diabetes curing is to keep normal glycemic levels. Type I diabetes is treated with parenteral

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd