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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Characters of vitamins: Vitamins are organic substances. They are micronutrients and are required in small quantities. They are not synthesised in the body and therefo
Cilia and flagella are structures found in various prokaryotes as well in some eukaryotic cells. They play defense, nutrition and movement roles for the cell. In eukaryotic cells o
Q. What do you mean by Myocarditis? Myocarditis is defined as inflammation of the myocardium. The most common cause is Coxsackie B virus infection. But it can also be due to
Vitamins Vitamins, as you may already know, derive its name from the word 'Vital Amine'. These are the organic compounds required for normal metabolism, growth and developme
Q. What are the proteins? How can the protein diversity of living beings be described? Proteins are molecules made of sequences of amino acids bound by a peptide bond. The g
Non-Surgical Therapy The most conservative approach to treatment involves non-surgical therapy. This treatment modality includes three subcategories: a) Pharmacological ther
What is the primary structure of a protein? What is the importance of the primary structure? Ans) The primary protein structure is the linear sequence of amino acids that produc
NAILS - Cover terminal segment of fingers & toes. Highly vascular dermis under lying the nail is called nail bed . It gives pinkish colour to nail. The small part
Zygote - Embryogenesis The fertilized egg or zygote is situated at the micropylar end/pole of the embryo sac, its basal (micropylar) end is attached to the embryo sac wall and
Define Burette - Nutritional Biochemistry A burette is a vertical cylindrical piece of laboratory glassware with a volumetric graduation on its full length and a precision tap,
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