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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Give number of valence electrons for technetium. Write out electronic configuration for the valence electrons. Give the number of valence electrons and the number of electronic con
Chitra Valve : This valve was designed and developed at Sri.Chitra Institute of Medical Sciences in Thiruvananthapuram and marketed by TTK Industries. It is a tilting di
What are sarcomeres? Sarcomeres are the contractile units of the muscle tissue formed of alternating actin blocks (thin filaments) and myosin blocks (thick filaments). Various
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What do you understand by Mesocoel? The middle of three coelomic spaces found in tripartate body plan characteristic of deuterostome lineage of animals. Other coelomic compartm
Why do the sons of a haemophiliac father never vary from this trait? Why is the cleavage in mammals referred as simple homoplastic?
Complete feed system in ruminants Complete feed system for feeding dairy animals is a departure from conventional individual rationing method. It involves the self-feeding of
Q. Economic aspect of biodiversity? Biodiversity serves as an income generating activity for countries around the world. Many people visit forests, beaches, mountains, grasslan
What do we mean by the term isomer? Existence of different compounds having same molecular form but dtfferent structural forms are isomers. Monosaccharides exhibit a varie
In phylum colenterata if tissues are present in the body of an organism then why organs are not present as group of tissues makes an organuestion..
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