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You may have heard in the news currently that a new species of frog was discovered within a rainforest.
You objective are to isolate genomic DNA and construct an expression library by using λ phage. You are interested within cloning the gene which encodes an enzyme that produces an intense green skin color and also you have an antibody which you think will work.
Questions:
a) Design a well controlled experiment to illustrate that your antibody will work for the library screening. Sketch a picture of your expected experimental effects as part of your reply.
b) Propose two reasonable explanations why you may not be capable to isolate the DNA even if you plated three genomes worth of DNA into your screening.
Technique : The suitability of the pulmonary autograft for aortic valve replacement has to be studied by accurate measurement by echocardiogram of the aortic and pulmonary annuli.
Signal transduction occurs when an extracellular signaling molecule activates a cell surface receptor. In words, this receptor change intracellular molecules changes a response. Th
what is the structure of hiv virus?
A patient was complaining of frequent urination, excessive thirst and dehydration. His fasting glucose level was found to be normal. Name the disease and its cause. Describe
State the definition of Bio-medical waste 'Bio-medical waste' means any solid and/or liquid waste including its container and any intermediate product, which is generated durin
Explain the Canal Obstruction File work shorter than the normal apical and feel a great resistance to reach true length. a. A retained instrument o Canal calcification b.
Q. Factors Affecting Water requirements of microorganisms? Factors that may affect aw requirements of microorganisms include: 1. Kind of solute employed to reduce the aw: F
Q. How are gametes formed in the pteridophyte life cycle, by mitosis or meiosis? What is the type of meiosis that occurs in pteridophytes? In pteridophytes gametes are made by
Explain the Chemical Properties of Monosaccharides? As you already know the chemical properties of monosaccharides depend on the presence of the hydroxyl the aldehyde or the ke
Dextrins Dextrins are also polymers of D-glucose held by a(l,4) glycosidic linkages. Dextrins are, in fact, formed due to partial hydrolysis of starch by enzymes such as sa
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