How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add

Related Discussions:- How much template dna will you add

Explain briefly protein hydrolysates, Explain Protein hydrolysates It i...

Explain Protein hydrolysates It is valued for their superior nutritional qualities, including increased bioavailability and reduced antigenecity. Several enzymatic modification

Is nitrogen is important plant nutrients, Is nitrogen is important plant nu...

Is nitrogen is important plant nutrients Nitrogen is an important plant nutrient which is assimilated by most of the plants as nitrate and ammonium ions. Organic matter is the

Explain the structure of fungi, Explain the Structure of Fungi? The veg...

Explain the Structure of Fungi? The vegetative structure of a fungus is called thallus. It varies in complexity and size, ranging from unicellular yeasts to multicellular mould

Explain fixer1 split s in second heart, Explain Fixer1 Split S in second he...

Explain Fixer1 Split S in second heart ? A2P2 interval is wide and persistent and doesn't change during respiratory cycle. It is an auscultatory hallmark of uncomplicated ostiu

Transport system in higher animals, Transport system in higher animals ...

Transport system in higher animals The circulatory system in higher animals has undergone several changes during evolution. The heart has become totally muscular. It p

Explain the methodology for seliwanoff test, Explain the Methodology for se...

Explain the Methodology for seliwanoff test? Take 5 ml of seliwanoff's reagent in a test tube. Add 5-6 drops of the glucose solution and heat the mixture to boiling for about 3

Scope of biology, SCOPE OF BIOLOGY- Present century is witnessing ex...

SCOPE OF BIOLOGY- Present century is witnessing explosion of information in modem biology directly related to life and health. Issues like global warming, pollution, populat

Unit three biology AS level coursework, I need help with my AS level corsew...

I need help with my AS level corsework

What are the major theoretical models, Q. What are the major theoretical mo...

Q. What are the major theoretical models that try to explain the formation of the enzyme-substrate complex? There are two major models that explain the formation of the enzyme-

Describe in brief about pseudoisoehromatic plates test, Describe in brief a...

Describe in brief about Pseudoisoehromatic Plates test Commonly known as Ishihara's plates, these reveal one pattern to the normal eye and another to the colour deficient. Colo

Write Your Message!

Name

Email id

Message

Verfication Code

User Account

All Pages