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You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add
What volume of 4x protein sample buffer do you need to add to 15 ml of a cell extract in order to load it on a gel?
In the lack of tryptophan shown in the figure, a trp repressor protein encoded by a split operon, trpR that is synthesized and forms a dimer. Furthermore, this is inactive and so
Regeneration - Root and Shoot Morphogenesis Higher plants have three kinds of regenerative activities; All plants possess inherent ability to regenerate lost parts. Fo
Impoundments - Lentic Ecosystems We have so far discussed natural lakes. In addition to these there are a number of lakes both small and large artificially created by man cal
Q. Mismatch and Recombinational repair? Mismatch repair: 1. Mismatches can take place when DNA polymerase inserts wrong nucleotide during replication 2. Mismatch repair is
Phototherapy As you know exposure of the jaundiced infant to blue, white or green light is effective in reducing the serum billirubin level. When bilirubin absorbs light, ser
Plankton - Aquatic Ecosystem This group includes both microscopic plants (phytoplankton) and animals (zooplankton) found in all aquatic ecosystems, except certain swift moving
diff b/w protonephridia and metanephridia
how to make a assignment on recent research on marine microbiology
What do you understand by Open circulatory system? A circulatory system in which circulating fluid (blood) flows into vessels or tubes not connected to each other by small capi
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