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You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add
Ask que1. Design a 4 colour Flow cytometry experiment with an aim to identify B220-, CD3+, CD4+ (DOUBLE POSITIVE) AND B220+, CD3-, CD4- (DOUBLE NEGATIVE) cells. Select from the fol
Write two cytological approaches in taxonomy.
Spina Bifida Occulta There is no protrusion of the meninges but the vertebrae are bifid. There are three components of the spina bifida occulta. a) Vertebral- there is
what is a seed?
Define the Public Nutrition Public nutrition is concerned with improving nutrition in populations in both poor and industrialized countries, linking with continuity and public
Q. What is the concept of universality of the genetic code? What are the exceptions to this universality? The genetic code is universal because the rules of protein codificatio
CARDIO PULMONARY BYPASS : Open-heart surgery is considered as one of the most significant advances in medicine of 20th century. Establishment of safe cardio pulmonary bypass (CP
What is the greenhouse effect? The greenhouse effect is a phenomenon in which atmospheric gases like CO 2 trap reradiated heat from the Earth, much as the glass panes of a gre
Define Food Business and Food Technology? The food business and food technology are practically inseparable. The food business may be characterized as: vulnerable to spoilage,
Explain why the chance of fertilisation in humans is restricted to only a few days each month. Sperms can fertilise an ovum for up to about three days after entering the female
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