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You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add
Describe about the stomach
A cDNA library is made by using the reverse transcriptase of a retrovirus to synthesize cDNA (complementary DNA) copies of the total mRNA from a cell (or perhaps a subfraction
Define drawbacks of Nutritional status that assessed traditionally? Nutritional status assessed traditionally with the use of anthropometric, biochemical clinical and dietary a
Mr. Smith read that cholesterol was bad for his health so he eliminated all foods and food products containing this molecule. He later found that his cholesterol level dropped only
Which of the following best defines the reasons why the sliding clamp is able to confer processivity on DNA polymerase and not DNA primase? A. The sliding clamp coats the templ
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Radiation Pollution It is unfortunate that harmful radiations cause severe pollution but they cannot be seen or observed by eyes and are difficult to judge, unless present in
Q. How are artificial and natural pearls created? Pearls are made from small strange particles that deposit between the mantle and the shell of the oyster. These particles trig
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Endocrine glands Endocrine glands have no ducts - so these are also called as ductless glands Eg.Pituitary etc., These glands secrete chemical substances called HORMONES
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