How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add


Related Discussions:- How much template dna will you add

Define the term- new life for ancient dna, Based on your reading of the art...

Based on your reading of the article "New Life for Ancient DNA" which of the following is a  false statement in regards to the comparison among genes in extinct and living species?

What is the function of the left ventricle, What is the function of the lef...

What is the function of the left ventricle? Where does the blood go after leaving the left ventricle? The function of the left ventricle is to get blood from the left atrium an

Discuss important factor of obesity, Discuss important factor of obesity ...

Discuss important factor of obesity Weight is a most important factor for identifying obesity. Weight is measured in kilograms (kg). It is measured during the first visit and n

Lithosphere, The lithosphere contains all of the hard solid land of the ear...

The lithosphere contains all of the hard solid land of the earth crust and the semisolid land underneath the crust. The surface of the lithosphere is very uneven contains high moun

Define the g1 phase during their cell cycle, What would happen to a cell an...

What would happen to a cell and its offspring if the cells did not go by a G1 phase during their cell cycle? Describe Without a G1 growth phase, the cells would not grow to th

Water cycle-hydrological cycle, Water Cycle (Hydrological Cycle) The mo...

Water Cycle (Hydrological Cycle) The movement of water on the earth is continuous and forms many complex inter-related loops (Figure shown below). Cycling of water involves atm

What is market in management term, What is market in management term? ...

What is market in management term? A market: There is Buyers and sellers for a good or a service come within contact for the function of normally exchange, for money. Lik

Starch, why is it necessary to grind the food samples before testing?

why is it necessary to grind the food samples before testing?

Explain full liquid diet, Explain Full Liquid Diet Full Liquid Diet:  ...

Explain Full Liquid Diet Full Liquid Diet:   This diet provides foods and fluids that are liquid or semi liquid at room temperature. The type of food provided may vary dependi

Lipids - digestion process, Lipids are essential fats that have much import...

Lipids are essential fats that have much importance to the human body. Lipids are biological molecules that are insoluble in aqueous solutions and soluble in organic solvents are c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd