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You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?
Surgical Therapy The clinician may use a surgical approach when non-surgical therapies are not indicated or are unsuccessful. The surgical techniques are modified from those
Q. Photosynthesis is the most significant producer of molecular oxygen (O2) on our planet. From which molecule do oxygen atoms liberated by photosynthesis come and from which other
Define about the Listing Plane Listing proposed that only horizontal movements of the eye are performed around a vertical axis in the equatorial plane and vertical moveme
The active transport of molecules requires an input of metabolic energy and this can be derived either from direct coupling to the hydrolysis of ATP or by coupling to
Temperature - Estuaries Another important variable is temperature. The temperature of estuary keeps on fluctuating it heats up and cools down more rapidly under prevailin
Explain about the Chytridiomycota - Fungi Chytridiomycota - Look at the Figure, which illustrates chytridiomycota. These are the simplest among true fungi and are commonly call
Occurrence of Jaundice Excessive production of bilirubin - this occurs in some diseases due to destruction of large number of red blood cells in the liver. Obstruction to
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Q. What is the function of the pupil and of the iris? The iris works like the diaphragm of a photographic camera since it has muscles that relax or contract varying the pupil d
Explain the Compartments of Body Water? Within the body, water is found in two major compartments. These are: - The intracellular compartment (inside the cell) - The extr
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