How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

Draw a graph for an iv multiple infusion of a drug, Draw A graph for an IV ...

Draw A graph for an IV multiple infusion of a drug, and label on the graph where the trough (predose) blood draw would be collected and where the peak (postdose) blood draw would b

#title.circulatory system, #question.what is the mechanism of circulatory s...

#question.what is the mechanism of circulatory system .

What information must be included on the form, Submission of evidence items...

Submission of evidence items to the forensic laboratory requires that a specific "Evidence Submission Request Form" be completed. What information should be included on the form?

Biochemical changes - consequences of aging, Biochemical Changes - Conseque...

Biochemical Changes - Consequences of Aging Several biochemical changes are correlated with aging. Detailed biochemical studies in rats have been carried out by Prof. M.S. Kan

Deifition, what''s the define of mitochodria

what''s the define of mitochodria

Determine the use of water soluble vitamin A, Water soluble Vitamin A  ...

Water soluble Vitamin A  Water soluble vitamin A is a yellowish green, slightly turbid, fluorescent liquid of faint characteristic odour. The taste is at first faintly sweet an

Define lipids - tests for presence of exoenzymatic activity, Define Lipids ...

Define Lipids - Tests for Presence of Exoenzymatic Activity? Lipids are also high molecular weight compounds. Enzyme lipases (esterases) cleaves the ester bond to form glycerol

Zoology, what are the physical requirements to become a zoologist?

what are the physical requirements to become a zoologist?

Vertibrates, what is the classification of vertibrates

what is the classification of vertibrates

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd