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You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?
Q. Describe Infection control sterilization protocol? Today is the age of modern technology coupled with medical challenges. Pervasive increase in serious transmissible disease
who is the first person who saw the cell
Explain the process of diagnosis of cholera Diagnosis: Cholera can be confirmed only by the isolation of the causative organism from the diarrheic stools of infected individual
Define Micronutrient needs of a lactating mother? Vitamin and mineral deficiencies can have profound influence on the composition of milk. Calcium is a nutrient of special conc
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What is Mefloquine Mefloquine is taken once weekly, but recent reports of serious psychiatric adverse effects have prompted the manufacturer, along with the FDA, to strengthen
Direct demonstration of the causative agent by electron microscopy (EM): Where facilities for electron microscopy are available and a viral disease is suspected, presence o
Explain for Procedure Quantitative Determination of Viable Microbes? Now carry out the exercise following the steps included herewith: 1. Label the diluent tubes from 10 -1
Mrs. TT seventy three-year old has a long history of chronic obstructive pulmonary disease (COPD). She came to the medical ward as shortness of breath, wheezing and a cough for the
Q. What are the major characteristics of the bryophytes? Bryophytes are nonvascular plants that is they do not have conductive tissues and they perform transport of nutrients a
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