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You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?
Formation of lactate and its consumption Formation of lactate and its consumption : If anaerobic conctitions prevail, the reoxidation of NADH through the respiratory cha
Q. What are the major components of the cytoskeleton? The cytoskeleton is a network of very small filaments and tubules distributed throughout the cytoplasm of eukaryotic cells
Explain the Colorimetric Method Glucose Estimation? Alkaline copper reduction methods are by far the most commonly used methods for glucose estimation. This time honoured metho
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Explain Fontan Operation ? The original Fontan operation was done in a case where classical Glenn operation had been done (end-Lo-end anastomosis of superior vena cava and lig
Classification of Living Organisms The world of living organisms is extremely diverse. Biologists call these diverse forms 'species'. It is estimated that over fifteen lakh (1
Pollen Tube Structure The pollen tube in the stigma is filled with cytoplasm containing numerous mitochondria and dictyosomes. The number of dictyosome cisternae is reduced i
CELL WALL Discovered by Robert Hooke , 1665 when he saw dead empty cork cells. Bonne r - studied the chemical nature of cell wall. The cell wall is a rigid & prote
Explain the primary function of blood? The primary function of the blood to transport oxygen from the lungs to body tissues for interior respiration. The blood helps in maintai
Define Use of Isotopically Labelled Nutrients? Nutrient Turnover Radioactive labelled nutrients are used to know the total body pool and the compartment in which it is stored.
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