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You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?
Q. Mismatch and Recombinational repair? Mismatch repair: 1. Mismatches can take place when DNA polymerase inserts wrong nucleotide during replication 2. Mismatch repair is
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