How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

Explain various types of microorganisms, Q. Various types of microorganisms...

Q. Various types of microorganisms? Microbes are single-cell organisms so tiny that millions can fit into the eye of a needle. They are the oldest form of life on earth. Micro

Why diabetics patient should have vegetables every day, Why Diabetics patie...

Why Diabetics patient should have Vegetables every day Diabetics should have vegetables every day. Fresh vegetables are to be preferred because they have less fats and salt. Da

Define physiologic functions of iodine, Define Physiologic Functions of Iod...

Define Physiologic Functions of Iodine? Thyroid hormone performs multiple functions as regulator of cell activity and growth. The hormone has crucial metabolic roles in the foe

Describe open circulatory system, Q. What is an open circulatory system? ...

Q. What is an open circulatory system? Open circulatory system is the one in which blood doesn't circulate only inside blood vessels but it also falls in cavities that irrigate

Define fluids required for underweight - nutritional care, Define Fluids re...

Define Fluids required for underweight - nutritional care? Take fluids only after a meal instead of with or before meals so that food intake is not reduced. High calorie nouris

Determine uses of soybean protein, Determine uses of Soybean protein? S...

Determine uses of Soybean protein? Soybean protein concentrates have been used as stabilized dispersions in milk- like beverages and simulated dairy products, such as sour crea

Monocot and dicot plants, project formate for monocot and dicot plants in f...

project formate for monocot and dicot plants in feild per month

What is st heart rate index, Q. What is ST Heart Rate Index? To calcula...

Q. What is ST Heart Rate Index? To calculate the index, the maximum ST depression is divided by the Delta heart rate (difference between the resting and maximum heart rate). As

Explain the origins of replication, Normal 0 false false fa...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain procedure for sub-culturing of a culture, Explain Procedure for Sub...

Explain Procedure for Sub-Culturing of a Culture Now carry out the exercise following the steps given herewith: 1. Sterilize the inoculating loop and needle by heating to re

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd