How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

Agro industrial-evaluation of feedstuff, Evaluation of feedstuff In th...

Evaluation of feedstuff In the feed manufacturing process evaluation of the feed ingredients is very important in achieving consistent quality throughout the year. If one is a

Calculate the electric potential, The three charges in the are at the verti...

The three charges in the are at the vertices of an isosceles triangle. Let q = 7.00 nC and calculate the electric potential at the midpoint of the base.

Adult (post natal) stem cells-types of stem cells, Adult (Post natal) stem ...

Adult (Post natal) stem cells : act as repair system for the body replenishing specialized cells but also maintain the normal turnover of regenerative organs such as blood, skin o

Cellular machinery for the replication of nucleic acids, Which of the follo...

Which of the following statements is true? Answer Viruses are distict from cells by the absence of a lipid membrane around them. Viruses are parasites that require the cell's metab

Byproducts high in energy and nitrogen, Byproducts high in energy and nitro...

Byproducts high in energy and nitrogen Byproducts in this category include products such as blood meal and poultry offal meal, fish meal, extracted oilseed meals/cakes and wast

Define the food system in space, Define the Food System in space? Mercu...

Define the Food System in space? Mercury (1961-1963) astronauts had to eat bite-sized cubes, freeze dried powders, and semi liquids stuffed in aluminium tubes. For most asbonau

Dna replication, DNA Replication Deoxyribonucleic acid (DNA) is the car...

DNA Replication Deoxyribonucleic acid (DNA) is the carrier of genetic data for all living creatures. An organism's genome, made of DNA, encodes the genetic blueprint for buildi

Micro biology, explane the role of nitrogenase enzyme

explane the role of nitrogenase enzyme

Explain glutathione peroxidase, Explain Glutathione peroxidase Glutathi...

Explain Glutathione peroxidase Glutathione peroxidase is a natural antioxidant present in many tissues. Together, with vitamin E it  is part of the body's  defense against lipi

Define chronic fever and infection, Chronic fever and  infection Chron...

Chronic fever and  infection Chronic fever  and  infection are always supported will a long standing history of symptoms  or repeated episodes of infection. One of the commonl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd