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You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?
Explain Ventricular Fibrillation (VF) and Pulseless Ventricular Tachycardia (VT) The commonest rhythm seen in cardiac arrest is VF, which may be preceded by a short period of
Explain the term of Monosaccharides? Monosaccharides : The simplest carbohydrates are Monosaccharides, simple sugars. These generally have three to six carbon atoms, and con
Q. Diagnosis of diabetes mellitus? Timely and proper diagnosis plays a key role not only in identifying new cases but also in managing old cases with or without diabetic compli
How does the hypophysis-corpus luteum negative feedback work? What is the name given to the atrophied corpus luteum after this feedback process? After ovulation the estrogen an
Define Factors Which May Lead To Errors in Count? There are many factors, which may lead to errors in count. (i) Counts may be low if the agar medium used in the technique d
Explain the Concept of Menu Planning? You have learnt from the previous units that our body's many functions, be it physical activity, mental exercise, growth (in the case of c
Regulation of HMP Pathway The following factors play an important role in regulation of HMP pathway: a) The first reaction of this pathway catalysed by glucose-6-phosphate
Define the meaning of value - Soil Value refers to relative lightness or darkness of a colour and is a measure of the amount of light that reaches the eye under standard lighti
Admission Procedure From the time the child and his family enter the hospital doors, the admission procedure should be carried out in a friendly and efficient manner. Admiss
Q. How do malign neoplasias appear? The Neoplasias appear due to DNA mutations in genes that regulate the cellular proliferation thus making the cell lose its capacity to contr
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