How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

The genetic code within a dna molecule, How is a genetic trait determined b...

How is a genetic trait determined by the genetic code within a DNA molecule? A DNA molecule has 4 dissimilar bases, either CGTA. Any specific combination of these things forms

Complications during angina pectoris, Q. Complications during angina pector...

Q. Complications during angina pectoris? It is a symptom giving a warning of impending myocardial infarction, sudden cardiac death or even ischemic necrosis of the brain leadin

Replication of chromatin, Whenever a chromosome is replicated the replicati...

Whenever a chromosome is replicated the replication machinery passes by the nucleosomes without deleting the histones from the DNA. How this happens is since not fully understood.

Tree, what is a seed?

what is a seed?

Different types of ecosystem, An ecosystem is a self sufficient interacting...

An ecosystem is a self sufficient interacting system in the biosphere. Eg. forest, desert, pond etc. almost, all the ecosystem have more or less similar fundamental plan for their

Chemical element is the central in the chlorophyll molecule, Q. Which chemi...

Q. Which chemical element is the central in the chlorophyll molecule? The chemical element that is the central in the chlorophyll molecule is magnesium. One atom of magnesium

What are sensory receptors, What are sensory receptors? Sensory recepto...

What are sensory receptors? Sensory receptors are structures specialized in the acquiring of information, such as temperature, mechanical pressure, pH, chemical environment and

Imaginal discs, Imaginal Discs In the holometabolous larva, there are ...

Imaginal Discs In the holometabolous larva, there are two cell populations: (1) The larval cells that are used for the larval structures and (2) The imaginal disc and th

Define the symptoms in bone marrow of pernicious anaemia, Define the sympto...

Define the symptoms in Bone marrow of pernicious anaemia? The nucleated red cells of the marrow are greatly increased.  The successive nucleated cell stages in erythropoiesis a

Endosperm with micropylar and chalazal haustoria, Endosperm with Micropylar...

Endosperm with Micropylar and Chalazal Haustoria Some plants develop haustoria from both micropylar and chalazal ends of endosperm. In Nemophila aggressive haustoria arise fr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd