How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

When did life appear on earth?, When did life appear on earth? It is es...

When did life appear on earth? It is estimated that the life on earth emerged about 3.5 billion years ago, thus 1 billion years after the formation of the planet.

Population and sigmoid curve, Name two possible why the number of live bact...

Name two possible why the number of live bacteria cell have reached the stationary growth by 60hrs and start to die off after 12hrs?

Signify the glands which are located at upper end of kidney, Part of the en...

Part of the endocrine system in humans, these 2 glands are small bodies located at upper end of every kidney. While these glands perform a range of functions, two of the most signi

Vasodilatation from the time of injury, The blood vessels surrounding an ac...

The blood vessels surrounding an acutely inflamed site usually represent progressive vasodilatation from the time of injury.

Sarcolemma, Normal 0 false false false EN-IN X-NONE ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What is pleiotropy, What is pleiotropy? Pleiotropy (or pliotropy) is th...

What is pleiotropy? Pleiotropy (or pliotropy) is the occurrence in which a single gene conditions several dissimilar phenotypical traits. Some phenotypical traits might be s

Determine the fructose to glucose ratio in honey, Q. Determine the fructose...

Q. Determine the fructose to glucose ratio in honey? This activity will help you to: • check the given samples of honey for any adulteration with commercial sugar

Explain the food preparation and cooling - canning, Explain the Food prepar...

Explain the Food preparation and Cooling - Canning? Food preparation - Preparation methods will change with the type of food. For fruit, cutting to size, washing, grading, peel

Treatment and nursing management of cleft lip, Treatment and Nursing Manage...

Treatment and Nursing Management The initial treatment is surgical repair of  the cleft. The timing of the repair varies from surgeon to surgeon, but most prefer cleft lip rep

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd