How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

Biology Question, Why is VeggieTales such a consistently high-quality progr...

Why is VeggieTales such a consistently high-quality program?

Vacuoles, VACUOLES They are of 4 types - 1.       Sap Vacuole - ...

VACUOLES They are of 4 types - 1.       Sap Vacuole - Outer membrane of sap vacuole is called T onoplast . Sap vacuole contains amino sugars, carotenoids, proteins, m

Lens regeneration in amphibians, Lens Regeneration in Amphibians It i...

Lens Regeneration in Amphibians It is an amazing fact that between all vertebrates only certain amphibians have the unique ability to regenerate lens, iris and retina in adul

State the disorders of sleep, State the Disorders of sleep The need for...

State the Disorders of sleep The need for sleep varies considerably from one person to another, as well as in the same person at different stages of life. We have all been told

Odyssey, how does odysseus get the dead to talk to him?

how does odysseus get the dead to talk to him?

Ringworm, R i n g w o r m It is also known as dermatophytosis re...

R i n g w o r m It is also known as dermatophytosis resulting in alopecia, scab formation and ring like lesions in the affected body parts. Eti o logy: Zeop

Define fluorides metabolism, Define fluorides Metabolism? Soluble fluor...

Define fluorides Metabolism? Soluble fluorides, even at high intake levels are almost completely absorbed from gastrointestinal tract. These include aqueous solutions of fluori

Inferior epigastric artery-other arterial conduits, Inferior Epigastric Art...

Inferior Epigastric Artery (I.) :  This is a branch of external iliac artery supplying the abdominal wall. It is raised as a free graft for CABG. The usual length availab

What is the structure that maintains identical chromatids, What is the stru...

What is the structure that maintains identical chromatids bound? The structure that maintains identical chromatids bound is the centromere. Cell Nucleus Review - Image Diver

Respiration, lesson plan practical requirements

lesson plan practical requirements

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd