How much template dna will you add, Biology

Assignment Help:

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in your reaction. How much primer do you need to add? b) For the same reaction in (a), you have a stock concentration of template DNA that is 10 ug/mL. You want to add a total of 50 ng to your reaction. How much template DNA (volume) will you add?


Related Discussions:- How much template dna will you add

Explain the term- horizons, Explain the term- Horizons These processes ...

Explain the term- Horizons These processes take a long time- may be a few thousand years to create a soil. As the time passes, the soil matures and generally becomes deeper and

Developmental biology, a sea urchin egg is injected with an mRNA encoding t...

a sea urchin egg is injected with an mRNA encoding the cadherin protein fragment described above and an mRNA encoding the Pmar1 transcription factor. The embryo is allowed to devel

Explain about the optic disc, Explain about the optic disc The optic di...

Explain about the optic disc The optic disc is also called a blind spot while the macula is referred to as the yellow spot. The ora serrata (the intersection between the retina

Development of genetics-a historical perspect, DEVELOPMENT OF GENETICS-A HI...

DEVELOPMENT OF GENETICS-A HISTORICAL PERSPECT As systematic study of genetics began, answers were sought to questions such as i) why are we like our parents? ii) why do org

Describe class ophiuroidea in general way, Describe Class Ophiuroidea in ge...

Describe Class Ophiuroidea in general way? Brittle stars have a central disc with five long, thin, flexible arms that can be detached from the body at will in times of danger.

What is community ecology explain their characterstics, What is Community E...

What is Community Ecology explain their characterstics? Community Ecology: In ecological terms, a community consists of an assemblage of all of the populations living and int

Oogenesis., write breif notes on oogenesis

write breif notes on oogenesis

#biology tutor title.., When nd how i can srt my online work in this site q...

When nd how i can srt my online work in this site question #Minimum 100 words accepted#

What is adaptive convergence?, What is adaptive convergence? The Adapti...

What is adaptive convergence? The Adaptive convergence is the phenomenon by which living beings facing the same environmental pressure (problems) and undergoing genetic variabi

BIN Report, Ask plquestion #Minimum 100 words accepted#

Ask plquestion #Minimum 100 words accepted#

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd