General procedure of titration - nutritional biochemistry, Biology

Assignment Help:

Define General procedure of titration - Nutritional  Biochemistry?

In general a titration requires that we add precise amounts of the known titrant to the unknown analyte or titrand. The usual setup is to use a burette, as illustrated in the margin figure. The titrant is placed in the burette and the volume noted on the graduations. This is the initial burette reading. A measured amount of the analyte is placed in a flask set below the tip of the burette as shown in the margin picture. The titrant is added slowly to the analyte by opening the stopcock and letting it flow into the flask.

By controlling the stopcock one can add titrant at any rate including drop by drop. The flask is swirled during addition to mix the components. When the endpoint is detected the titration is stopped. One must take care to slow down the addition of titrant as one approaches the endpoint to avoid overshooting it. At the endpoint the volume of titrant left in the burette is read. This is the final burette reading. The difference between the burette readings is the volume of titrant added to analyte. Since the titrant and analyte are present in stoichiometric amounts one can calculate the quantity of analyte from the titrant by a stoichiometric conversion which will be explained in every practical. The information we have read so far is very basic to our understanding of volumetric analysis. It is very important that you understand this concept clearly, primarily so because you shall be conducting the volumetric analysis in many of the experiments included in this manual. To help you consolidate your knowledge on this topic, we have included some review questions herewith. Answer these questions and recapitulate what you have learnt about the qualitative and quantitative technique.


Related Discussions:- General procedure of titration - nutritional biochemistry

Excretion, living organisms and their excretory products

living organisms and their excretory products

Procedures for diagnosis - polymerase chain reaction (pcr), P o lymer...

P o lymerase chain reaction (PCR): The PCR technique enables the exponential amplification of nucleic acid sequences from any biological samples. In the last few ye

Define the stability of your favourite protein kinase, You measure the stab...

You measure the stability of your favourite protein kinase and find that half of the protein is degraded every 10 minutes. How might you test whether the protein is degraded throug

Explain the properties of gum arabic, Properties Gum Arabic is unique b...

Properties Gum Arabic is unique between the natural hydrocolloids because of its extremely high solubility in water and can yield solutions of up to 55% concentrations. Solutio

Prevention of iron deficient anaemia, Prevention   You as nurse needs t...

Prevention   You as nurse needs to ensure that iron supplementation  is given to children who are at risk for iron deficiency.  Iron supplementation  should start between 4 and

Agro industrial-processing of roughage, Processing of roughage Roughag...

Processing of roughage Roughages constitute the bulk of the ration of farm animals and these include green fodder (leguminous and non-leguminous), grasses, and various crop re

Define mechanisms for antinutritional effect on human, Define mechanisms fo...

Define mechanisms for antinutritional effect on Human? There are several possibilities: reduced availability of amino acids to cancer cells by reversing the carcinogen-induced

Canned and retort pouched meat products, C a nned and Retort Pouched Meat...

C a nned and Retort Pouched Meat Products Canning as a process contributes to preservation, storage and distribution of food supplies. Production of thermally processed meat

Advantages of pyramid of energy, Advantages of Pyramid of Energy A pyr...

Advantages of Pyramid of Energy A pyramid of energy must be based on determination of the actual amounts of energy that individuals take in, how much they burn up during metab

Mixed aortic stenosis and regurgitation, Mixed Aortic Stenosis and Regu...

Mixed Aortic Stenosis and Regurgitation :  The combined aortic valve stenosis and regurgitation may be of congenital or acquired aetiology, as in aortic stenosis. Most common

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd