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Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.
How can the abundance and diversity of living beings in the tropical forests be explained? The biodiversity of these ecosystems can be described by the great availability of th
Research and Documentation - Conservation of wildlife First, list of endangered species are established by various national and international agencies. Another important actio
Types of Organization Chart: There are two important types of organization chart. a) Vertical i.e., from top to bottom. b) Horizontal i.e., from left to right.
Abnormalities in the lipid profile are found in patients with diabetes mellitus. The lipid abnormalities increase the risk of heart disease in diabetics and hence should be regular
Dictyosomes are produced on the Organelles in the plant cells composed of the series of flattened membrane sacs which sort, chemically modify, and package protein srough endoplasm
How to reduce calorie intake from fat? Efforts should be made to increase the nutrition knowledge of the general public through mass media. The foods with lower fat co
biological significance of biology
what is the classificatin of phylum protozoa with orders
Which of the following is not a difference among DNA primase and DNA polymerase? A. DNA primase can initiate replication of DNA de novo whereas DNA polymerase needs a short oli
Q What are the three periods into which interphase are divided? Interphase is the preceding phase to the mitotic division. It is divided into three periods, G1, S and G2 the le
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