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Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.
How many parts of human body?
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P l e u r iti s It is the acute or chronic inflammation of the pleural membranes. It is characterized by pain during respiration, pleural effusion, and shallow rapid resp
what are some adaptations of flatworms
Air pollution is complex in origin and varied in effect. It is very crucial from the point of human health. Every human being inhales about 15-22 kg of air daily and air is pollute
What is the fuction of Starch Starch is the main thickener in gravies, sauces and puddings. Starch absorbs water, and becomes a gel when cooked. As the starch swells up with w
Q How are the concepts of chromosome, chromatids and chromatin related? In which phase of the cell cycle does the DNA duplicate? Chromatin is a set of filamentous the DNA molec
Explain what is Light Microscope - Microscopy Modern light microscopes are compound microscopes. Here the magnified image formed by the objective lens is further enlarged by on
how they reproduce?
Chronic Renal Failure (CFW) Chronic renal failure begins when the diseased kidney can no longer maintain the normal chemical structure of body fluids under normal conditio
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