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Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.
Why is the determination of the blood types of the donor and of the recipient important in transfusions? The Red blood cells have different antigens in the outer surface of the
Boiling Point There are certain properties of solutions which are directly connected with vapour pressure and one of it is boiling point. You must have observed that water bo
Please answer the following two questions on Sequence X: 1) DNA sequence databases 1. What is the EMBL-Bank accession number for this sequence (use BLAST)? 2. Which gen
Define Tests of Cardiovascular Endurance? Cardiovascular endurance or aerobic fitness is the ability to exercise continuously for extended periods without tiring. It can be me
What is the mode of nutrition of Ascaris?
State the prevention of Campylobacteriosis Prevention: the various ways to prevent campylobacteriosis are: Wash hands before preparing foods. Wash hands immediatel
Explain the summary of morbidity in CAD? i) The prevalence of CAD among immigrant Indians is about three-fold higher than in comparable indigenous populations; ii) CAD tends t
Group specificity Some enzymes prefer a specific functional group to be present\%on the substrate molecules. Example:- alcohol dehydrogenase acts on alcohols having -OH gr
Foods with moderate amounts of protein can be eaten in limited amounts. These foods include grains, bread, pasta, rice, potatoes, corn, and peas. Foods with little or no protein ar
what are the origin, morphology, active constituents, uses and market preparation of tanco beans ?
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