Gene targeting in es cells, Biology

Assignment Help:

Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.


Related Discussions:- Gene targeting in es cells

Explain glucokinase, Glucokinase In the fed state, clearance of blood  ...

Glucokinase In the fed state, clearance of blood  glucose is mainly by  liver via glucokinase. Glucokinase, which  is an  inducible enzyme, removes most of the blood glucose fr

Vaccine against tuberculosis, Q. Is there vaccine against tuberculosis? ...

Q. Is there vaccine against tuberculosis? The vaccine against the tuberculosis is called as BCG (bacillus Calmette-Guérin). BCG is not used in a few countries where tuberculosi

Explain about the dietary fibre, Explain about the Dietary Fibre? You a...

Explain about the Dietary Fibre? You are all aware that fibre is an important component in the structure of plants. However, fibre as a dietaiy constituent was considered impor

Preparation of the standard curve for haemoglobin, Preparation of the stand...

Preparation of the standard curve for haemoglobin? Label 6 clean dry rest tubes and label them as S1 -S6 Add 5 ml of Drabkin's solution to test tube numbers S2 - S6

Define the concept of ventilation, Which of the following are true for vent...

Which of the following are true for ventilation? A. The problems with ventilation induced by injection of curare occur because of the drug's direct action on nicotinic ACh Rec

Heat production, Heat Production In the Homeotherms heat production m...

Heat Production In the Homeotherms heat production must be elevated if the ambient temperature falls below the critical temperature. Although all metabolic processes result i

Sterility in angiosperms, Sterility in Angiosperms Sterility in angios...

Sterility in Angiosperms Sterility in angiosperms may arise due to several causes. Either the anther may not be formed, or anthers are formed but meiosis may be abnormal. A

Glycogenesis, Glycogenesis :   This  is an anabolic process  in which  gluc...

Glycogenesis :   This  is an anabolic process  in which  glucose is polymerized  into glycogen by the   sequence of  reactions.  Since glycogenesis occur  in all  body  cells .but

What is hdls, HDLs have the opposite function to that of LDLs in which they...

HDLs have the opposite function to that of LDLs in which they deleted cholesterol from the tissues.  The HDLs are synthesized in the blood mainly from components derived from the d

Define the different causes of under nutrition, Define the different causes...

Define the different causes of under nutrition ? 1. Scales (Screening Tool): The basic biochemical and anthropometric parameters are used in this screening. 2. Recognizing th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd