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Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.
How do meiosis do in developing of new varieties of crops
NUCLEUS First described and christened by Robert brown 1831, a nucleus is the largest and most important organelle of eukaryotic cells. It is membrane bound and generally locat
Q. Explain nutritional management of metabolic diseases? The nutritional management of metabolic diseases such as gout and a few inborn errors of metabolism such as phenylketon
figure of contractile vacuole of amoeba
Domesticated animals provide much of the world's meat products today, but wildlife still constitutes to be an imp ortant source of food of many rural communities. The International
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DISORDERS OF LIVER: In the foregoing sections and sub-sections we have discussed about the common disorders of upper and lower gastrointestinal tract. Now we shall discuss
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