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Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.
What are the Consequences of PEM? The consequences of PEM are most often long lasting and irreversible. The common consequences include: • Irreversible growth retardation
Q. What is the difference between venous and arterial blood? Arterial blood is the oxygen-rich and carbon dioxide-poor blood that irrigates the tissues. Venous blood is the oxy
Define Precipitation or Solubility of Proteins? Most of the functional properties are dependent on the degree to which the proteins are soluble. The solubility behaviour provid
Which is the part of the human visual system where the receptors that sense light, i.e., the photoreceptor cells, are located? How do those cells work? The photoreceptor cells
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What are the main structures within the cell nucleus? Within the cell nucleus the major structures are: the nucleolus, an optically dense region, spherical shaped, where there
Q. Write in 3-4 lines about the term belief? Beliefs are learned by us when we were young. These include religious beliefs and beliefs about behaviour. Some beliefs lead to hea
Advantages of vegetative propagation Vegetative propagation helps to maintain fixed qualities and characteristic features of the parent plant. For Example in ornamental p
Pathophysiology The blood clotting mechanism occurs in three phases each dependent on preceding I phase. Due to the inherited deficiency of factor VIII and or factor IX, the
Explain the functis of the main organelles in plant and animal cell as seen under the lightmicroscope
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