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Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.
Special type of Wards: Additional accommodation or differences in size and shape of some of the special type of wards e.g. Intensive Care Ward, Infectious diseases ward and
Explain disease typhoid Typhoid is often called enteric fever because the infection or bacteria is found in the intestines and attaches itself to the epithelium of the intes
How do I use a pedigree charts
What is a vesicle? A vesicle is a relatively minute intracellular, membrane-enclosed sac that keeps or transports substances
Adrenal glands There are two adrenal glands one each on the top of each kidney. Adrenal gland has two parts, the outer cortex and inner medulla. Both cortex and medull
What is vernalisation? How is the process of vernalisation benefits to plants?
discuss about protozoa,metazoa,mesozoa and parazoa
Boiling Point There are certain properties of solutions which are directly connected with vapour pressure and one of it is boiling point. You must have observed that water bo
Adsk question #Minimum 100 words accepted#saliva enzyme
#questitypes of plant breeding on..
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