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Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.
Explain the Process of Canning? The basic principles of canning have not changed dramatically since Nicholas Appert and Peter Durand developed the process. Heat sufficient to d
Explain the Difficulty of establishing and sustaining collaborations? Working with another is both rewarding and challenging. Working across disciplines is even more so. Though
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Q. What are the fundamental constituents of the cell membrane? The cell membrane is formed of lipids, carbohydrates and proteins. The membrane lipids are phospholipids, a sp
Precautions to prevent from HIV To avoid HIV infection one should practice safe sex (use of condoms). Besides a few simple precautions are given for protection against HIV inf
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State the term - structural abnormality and unique behavioural The link between structural abnormality and unique behavioural aberration has become clearer as technological adv
Pneumonia Pneumonia is an inflammatory process in which there is consolidation in lung caused by exudates filing the alveolar spaces. Gas exchange cannot take place in the c
Explain about Skeletal muscle These are attached to the bones by tendons, which help in the movement of the part of the skeleton. These muscles are controlled by our conscious
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