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Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.
Prokaryotes and Eukaryotes By now, you know that cell theory holds good for all living forms. However, if you examine under the microscope, the cells of a bacterium, a blue gr
Q. What is the clitellum of earthtworms and where it is located? The clitellum is a special region of the annelid constituted by rings (metameres) with reproductive function. I
COMMON DISEASE OF PIG - 1 . FOOT AND MOUTH DISEASES 2 . ANTHRAX 3 . TUBERCLOSIS 4 . SWINE FEVER - Charactrized by high fever, d
Preventive steps for global warming : Growing trees in vacant places. Growing social forests and protect them. Promoting abundant growth of phytoplankton plant species in marine
How Rb protein's phosphorylation state affects the cell cycle and cancer progression. please put link or citation of where you find information.
The citric acid cycle, also called as the tricarboxylic acid (TCA) cycle or Krebs cycle (after its discoverer in the year of 1937), is used to oxidize the pyruvate making during th
in oogenesis only on functional egg cell is normally formed from primary oocyte. Briefly explain
Regulation of the Citric Acid Cycle The citric acid cycle is regulated by certain enzymes and by the availability of ADP.
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Where does hematopoiesis occur? Hematopoiesis happens in the bone marrow (mainly within flat bones), where erythrocytes, leukocytes and platelets are made, and in the lymphoid
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