Gene targeting in es cells, Biology

Assignment Help:

Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.


Related Discussions:- Gene targeting in es cells

What is consciousness, The importance of the brain in our everyday lives ca...

The importance of the brain in our everyday lives can never be underestimated. The brain has physical properties that are in a constant state of flux. The brain never rests totally

Mitral flow velotity, The normal flow pattern cross-mitral valve is a tall...

The normal flow pattern cross-mitral valve is a tall E wave where is due to early rapid filling and small A-wave which is due to atrial contraction. E/A ratio >1. Pulmonary

Explain the history of nutrition, Explain the History of Nutrition? Kno...

Explain the History of Nutrition? Knowledge of human nutritional needs and relationship between diet and health was placed on a modern scientific footing only since the 19th ce

Define consistency and texture - dysphilgia, Define Consistency and Texture...

Define Consistency and Texture - Dysphilgia? Aspiration of liquids, including water, into the lungs can result in complications. To avoid this problem and yet fulfilling the fl

Explain bacterial vaginosis, Bacterial vaginosis  The role of sexual tr...

Bacterial vaginosis  The role of sexual transmission is unclear in bacterial vaginosis, in which normal H2O2-producing Lactobacillus  species are replaced by overgrowth with  G

Define about the environmental deficiency of iodine, Define about the Envir...

Define about the Environmental deficiency of iodine? The ultimate causative Factor is deficient intake of iodine. Iodine occurs in soil and sea as iodide, the ions of which arc

Fast response - biological responses, Fast Response - Biological Responses ...

Fast Response - Biological Responses Some of the fast responses are discussed below: It was found that when mung bean root tips were kept in a specific solution (contain

What kind of agent causes sars, Q. SARS is a disease that appeared in 2003 ...

Q. SARS is a disease that appeared in 2003 with epidemic features in the province of Guangdong, in east China. What kind of agent causes SARS? SARS is caused by a virus from th

Give brief description about the generalised seizures, Generalised Seizures...

Generalised Seizures Generalised seizures are bilaterally symmetrical without focal onset. One subtype, the grand mal attack, is characterised by loss of consciousness and by s

Maintenance and regulation of peripheral circulation, Maintenance and Regul...

Maintenance and Regulation of Peripheral Circulation Pressure receptors (baroreceptors) located in the wall of the internal carotid arteries and in the arch of aorta, when

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd