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Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availability of large number of fertilized eggs and translucent zona. By contrast, the use of embryonic stem (ES) cells in the mouse has enabled us successfully in developing target specific modifications. When these cells with modified DNA are inserted into the early embryo, may contribute to the germline. They undergo a homologous recombination with transferred DNA of interest resulting in to precise and targeted changes in host genome. It is because of combination of these two properties, gene targeting including knockout of some undesired genes in mouse germline is a routine procedure. Despite extensive efforts globally, stable ES cells have not been isolated from livestock and this has constrained many potential applications for germline manipulation. Therefore, it was with some excitement that researchers in the field greeted the news that cloning by nuclear transfer (NT) from cells grown in culture had been achieved as it pointed the way to the development of an alternative, cell-based, system for genetic modification.
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Leptospirosis Synonyms: Weil’ disease, canicola fever, 7-day fever Leptospirosis is an important and widespread zoonosis. It was first described by Adolf Weil in 1886 when h
What is reproductive isolation? The Living beings are considered under reproductive isolation when they cannot cross among themselves or if they can cross but cannot generate f
Plants of the open water zone In this zone plants are restricted to the limnetic one and generally consist of phytoplankton such as dinoflagellates, blue green algae and green
What are the uses of a barrier membrane is grafting procedures? Uses of barrier membrane in grafting procedures : i) Help in stabilizing the graft ii) Prevents ingrowth
Properties of pyridoxine a) It forms white, odourless crystals. b) The compound is readily soluble in water. c) When a neutral or alkaline solution of pyridoxine is
Q. Is there a larval stage in echinoderms? In echinoderms embryonic development is indirect, with ciliated larvae.
Counseling is a process whereby a qualified person purposefully assists another person to handle his or her problems. Counselling is not just giving advice. It is dependent on mut
How can an organism that once underwent contact with an antigen be immunized against future infections by the same agent? This phenomenon is known as immune memory . When an a
what is nitrogen cycle?
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