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Fluorescence MicroscopyCertain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.
Define Strategies for to overcome from alzheimer's disease? Some of these, based on the stage of the disease and individual needs are: a. supervising meal times with minimal
Gills - Respiration Gills are highly vascularised extensions of gas exchange membranes. The simple type may be extension of the body surface as in sea-slugs, sea stars and ma
A solute passes from higher concentration on the left of a container to the right side of container thru a membrane. What would happen if a second solute of lower concentration was
Cholesterol is a steroid. It is very significant constituentof cell membranes in mammals, it modulates their fluidity. Cholesterol is also the precursor of steroid hormones such
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What are the synthetic auxins and what are their uses? The Synthetic auxins like naphthalenic acid (NAA) and indolebutyric acid (IBA) are substances similar to IAA (a natural a
P a r a s i t i c Diseases F a s cioliasis It is a liverfluke infestation characterized by hepatic insufficiency, bile duct obstruction, and poor production per
Explain the Absorption, Storage and Elimination of Folate? Folic acid is readily absorbed from the small intestines through the portal vein and passed onto the tissues throug
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