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Fluorescence MicroscopyCertain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.
Define Factors that Affecting the Calcium Absorption? It is a well known fact that the amount of calcium that we eat need not be the amount of calcium that gets absorbed. The d
please i want detailed explanation on the mechanism of enzyme catalysis
A fatty acid having of a hydrocarbon chain and a terminal carboxylic acid group that is shown in the figure. Most fatty acids are in biology have an even number of carbo
Dextrins Dextrins are also polymers of D-glucose held by a(l,4) glycosidic linkages. Dextrins are, in fact, formed due to partial hydrolysis of starch by enzymes such as sa
What are sori? The word "sori" is the plural form of "sorus". In ferns, a sorus (pl. sori) is a cluster of sporangia on the edge or underside of a fertile frond. In lots of spe
How does the study of fossils strengthen the theory of evolution? The study of fossils reveals ancient and extinct species having many same structures to others of the present
Explain the term- Cerebral Ischemia Ischemia refers to any of a group of disorders in which the symptoms are caused by vessel blockage preventing a sufficient supply of blood t
Isomerases These enzymes (code EC 5) catalyze geometric or structural changes within a molecule. According to the type of isomerism, they may be called racemases
After Purchasing The following points should be considered for after purchase facilities. Avail of any training the software engineer or vendor may provide, even if it
Define Autoclave - Food Microbiology? For sterilization, steam under pressure is generally employed using an instrument called autoclave. Figure illustrates the autoclave. Auto
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