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Fluorescence MicroscopyCertain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.
Explain the Recommended Dietary Allowances - Nutrition? RDA: The RDA is the average daily dietary intake that is sufficient to meet the nutrient requirement of nearly all healt
Determine Pull-ups test for check the body strength? This test is also done to measure upper body strength or endurance by maximum number of pull-ups completed. Subject hangs f
Two chromosomes in a cell have identical length and centromere position yet are not homologous, what is different about them.
Characteristic Features of Phylum Arthropoda Well-developed muscular system along with striated muscles attached to the exoskeleton, and visceral organs having smoot
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Uterine Torsion Incidences of uterine torsions in cattle and buffaloes are not common. However, they are very common in heavy breeds and buffaloes and animals in slopy terrain
Q. Nutritional Management Goals for atherosclerosis? The nutritional management goals include: • Reduction of weight if overweight or obese • Reduction in the intake of
Q. Define Premotor Cortex Located in the frontal lobes? Premotor Cortex - Involved in control of more complex, learned motor skills like writing, driving, etc. Broca's area or
Define human beings need energy For Basal metabolism? This comprises a series of functions that are essential for life, such as cell function and replacement; the synthesis, se
Cytology: The study of form or culture including the behavior of nucleus and other organelles is called cytology. Cytology means "the study of cells". Cytology is the branch of lif
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