Fluorescence microscopy, Biology

Assignment Help:

Fluorescence Microscopy

Certain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.

The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.


Related Discussions:- Fluorescence microscopy

Explain the alpha-helix and the beta-sheet protein, Normal 0 fa...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Define the thin and thick filaments of a sarcomere, When the overlap betwee...

When the overlap between the thin and thick filaments of a sarcomere in a skeletal muscle is decreasing, A. the total length of the I band is increasing in the sarcomere. B.

Prolactin - vertebrates, Normal 0 false false false EN-...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Transplantation - clonal propagation, Transplantation - Clonal Propagation ...

Transplantation - Clonal Propagation The shoots or plantlets multiplied on a medium containing organic nutrients, show poor photosynthetic capability. Moreover, in these pla

Which kind of circulatory system do molluscs have, Q. Which kind of circula...

Q. Which kind of circulatory system do molluscs have? How can it be compared to the respiratory system of annelids? Molluscs, with the exception of cephalopods, have an open ci

Lymph, LYMPH - Lymph is a mobile connective tissue comprising lymph pla...

LYMPH - Lymph is a mobile connective tissue comprising lymph plasma (fluid) and lymphcorpuscles (cells).   COMPOSITIO N - (i )      Lymph Plasma - It is simil

Cilia and flagella – protozoans, Cilia and Flagella – Protozoans Cilia...

Cilia and Flagella – Protozoans Cilia and flagella basically have a similar structure and distinction between the two on structural basis does not exist. There is a filament o

Define primary motor cortex corticospinal interneuron a, Define Primary mot...

Define Primary motor cortex corticospinal interneuron A Primary motor cortex (M1) corticospinal interneuron A that produces action potentials during movements of the big toe of

Salivary analyse works on foods, Explain how salivary amylase works on food...

Explain how salivary amylase works on foods like crackers

Define the normal squid intracellular saline, At 1 AM, a researcher places ...

At 1 AM, a researcher places a healthy squid giant axon in a bath of normal squid physiological extracellular saline and internally perfuses the axon with normal squid intracellula

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd