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Fluorescence MicroscopyCertain compounds when exposed to short wavelength radiations, e.g., UV and X-rays. absorb and emit energy as light of a longer wavelength. This process of emitting light from a compound is known as fluorescence and is now commonly used in microscopy. Compounds which naturally emit light exist in most living tissues. For example, the mitochondria of liver and kidney, collagen and chlorophyll give a strong fluorescence. Such a type of natural fluorescence is called as autofluorescence. Sometimes fluorescent dyes llKe rhodamine, quinacrine etc. called fluorochromes are used to stain cell components to produce secondary fluorescence. Such important dyes are also used in the study of chromosome behaviour.The fluorescent microscope is essentially an ordinary optical instrument. It has been modified by the incorporation of special filters. These filters allow only the required wavelength of light to pass and cause fluorescence in the specimen. The most significant application of fluorescent microscopy is in the field of immuno-fluorescent,antibody labelling technique about which you will study later in this Unit.
Explain about the Secondary Protein Derivatives? These are the products of further hydrolytic cleavage of the protein molecule, Examples include: a) Proteoses: Soluble in wa
Cellulose is a polysaccharide which is composed of the unbranched chains of glucose; the major structural carbohydrate of the plants, insoluble in water, and indigestible in the h
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Define interaction of vitamin k with Calcium? Since vitamin D functions have an impact on calcium metabolism, an inter-relationship exists between vitamin IS-dependent proteins
What is the role of Linked Traits in genetics? In his study of inheritance in the fruit fly, Morgan observed that certain genes are distributed together, or linked, rather than
Define Transport Proteins in Plasma? Transport proteins, embedded in lipid membranes, make easy the import of nutrients into cells or the release of toxic products into the sur
Disadvantages of Protozoa
Explain the Failure of Endodontic Treatment 1. Microorganisms are left inside the canal - Improper/inability of cleaning and shaping, - Source of infection could be: peri
Ephemeral fever It is also known as three days sickness and affected animals suffer from pyrexia, muscular stiffness and lameness. Et i o l o g y :
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