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Explain Wire loop and Tube Method
1. Use a 25-gauge dental injection needle, cut to remove beveled end
2. Both ends of-mm diameter steel ligature wire passed through the needle from the injection end until slide out of the hub end, creating a wire loop that extends from the injection end of the needle
3. Inserted inside the canal & tighten around the separated file using small hemostat then withdraw.
organism that can not exist in the presence of oxygen ?
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Excretory organ for protozoa
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