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Explain Wire loop and Tube Method
1. Use a 25-gauge dental injection needle, cut to remove beveled end
2. Both ends of-mm diameter steel ligature wire passed through the needle from the injection end until slide out of the hub end, creating a wire loop that extends from the injection end of the needle
3. Inserted inside the canal & tighten around the separated file using small hemostat then withdraw.
Assume that you have 1 mL of a solution amylase (an enzyme) at a concentration of 15 mg protein/mL. Calculate the volume of diluting buffer that you would have to add to 1.0 mL of
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