Explain the determination of folic acid, Biology

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Determination

Reductive hydrolysis of folic acid produces 4-aminobenzoyl glutamic acid which is determined photometrically after diazotization and coupling with N-(1-naphthyl)-ethylenediamine.

In the microbiological assay, it is either by the turbidimetric growth measurement of Streptococcus faecalis ATCC 8043 or titrimetric determination of lactic acid produced by Lactobacillus casei ATCC 7469.

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