Explain Pure Culture Techniques

We learnt the techniques involved in sub-culturing, i.e., the process involved in transfer of culture from one medium to another or transfer of culture from the parent growth source to another. What is the role of sub- culturing? Basically, for maintaining and transferring the culture for various microbiological test procedures, sub-culturing as a technique is important. You would realize that microbiological studies require microorganisms in pure form. How to obtain these pure forms i.e., a single type of microorganism in a culture? This Practical introduces the concept of pure culture and the ways of obtaining it. After going through the practical, you will be able to understand the importance of obtaining pure culture in careful study of an individual microbial species.

Objectives

After studying this practical and conducting the exercises given herewith, you will be able to:  

• Explain the concept of pure culture,
• Describe the ways i.e., the methods employed in obtaining pure cultures, and
• Prepare and maintain pure cultures.