Explain Precautions for Gram Staining of Bacterial Cultures?

1. The dilutions should be chosen on the basis of expected counts in the food sample.

2. Food should be kept in the laboratory refrigerator or in cold till assay starts.

3. Food sample should be kept in a sterile container and should be collected aseptically.

4. Weighing of the sample should be done in a sterile stomacher bag (for stomacher samples) or into a sterile petri plate (for blender sample).

5. While using pour plate technique, do not remove all the media tubes simultaneously to avoid premature solidification of the medium.