Explain precautions for gram staining of bacterial cultures, Biology

Assignment Help:

Explain Precautions for Gram Staining of Bacterial Cultures?

1. Heat fix the smear, otherwise cells would be washed off.

2. Fresh 24 hrs old culture should be used. Gram variability can be seen in old culture. Since with age gram Positives lose their ability to retain primary stain, in gram variability some cells appear purple and others appear red.

3. Decolourization is a critical step. Decolourization is done till the colour of the primary stain comes out. Over-decolourization can make gram positives appear gram negatives and under decolourization can cause gram negatives to appear as gram positives.

4. Slide should be thoroughly washed after every step to remove excess reagent under running water or water applied with an eye dropper.

 


Related Discussions:- Explain precautions for gram staining of bacterial cultures

What is the inactivation of the x chromosome, What is the inactivation of t...

What is the inactivation of the X chromosome? What is a Barr body? Inactivation of the X chromosome is a phenomenon that happens in women. As women have two X chromosomes only

Mention the main cause of air pollution in metro cities, a) Mention the mai...

a) Mention the main cause of air pollution in metro cities. Write any three ways by which it can be decreased. b) How did Eli Lilly synthesize the human insulin? Mention

State the types of bone density, State the types of bone density Misch ...

State the types of bone density Misch classified bone density into following types (1988) D1. Dense cortical bone- which is almost never observed in maxilla and approximatel

Chromosomes and molecular homology, CHROMOSOME S AND MOLECULAR HOMOLOGY - ...

CHROMOSOME S AND MOLECULAR HOMOLOGY - The DNA content of diploid cells is similar. Hoyer et al in 1965 showed that DNA of humans was 100% similar with chimpanzee and 94%

Antioxidants and flavanoids requirement in dyslipidemia, Q. Antioxidants an...

Q. Antioxidants and Flavanoids requirement in dyslipidemia? Antioxidants and Flavanoids: You must have already read about different antioxidants present in our foods. The body

What is the treatment for hiv, Anti-retroviral drugs are given to the patie...

Anti-retroviral drugs are given to the patient. They lesser the viral load and gives relief from infection, but it is not lasting it is temporary relief i.e. it cannot cure.

Analytic characters - nature and structure of community, Analytic Character...

Analytic Characters - Nature and Structure of Community As you know a community has its own characteristics. Which are not shown by its individual component species. These cha

Will there be an opportunity to comment, Will there be an opportunity to co...

Will there be an opportunity to comment?  In a supplemental notice accompanying the last rule package, EPA is soliciting additional information and public comment on issues fro

Concepts and definitions in relation to nutrient requirement, Concepts and ...

Concepts and Definitions in Relation to Nutrient Requirements 1) The probability concept describes the relationship between the levels of intake and the probability of risk of

Temperate evergreen forest - ecosystem, Temperate evergreen forest - Ecosys...

Temperate evergreen forest - Ecosystem Many parts of the world have a mediterranean type of climate which is characterised by warm, dry summers and cool, moist winters. These

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd