Explain precautions for estimation of reducing sugar, Biology

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Explain Precautions for Estimation of Reducing Sugar by Fehling Soxhlet Method?

1. Clamp the burette so that the tip of the burette is exactly above the mouth of the conical flask.

2. Maintain continuous evolution of steam by continuous heating of the conical flask to prevent reoxidation of Cu⁺ ions.

3. Each titration should be completed within three minutes.

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