Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

Effects of environmental disasters, Primary effects 1.      Nuclear con...

Primary effects 1.      Nuclear contamination 2.      Biological contamination 3.      Chemical contamination 4.      Destruction of infrastructure facilities. 5.

What type of building block is atp, Please show a diagram of the ATP molecu...

Please show a diagram of the ATP molecule and label the major parts ot this molecule. Also, what kind of building block is ATP?

Implications of pharmacogenomic research, Problem 1: Describe the funct...

Problem 1: Describe the functions of: a. Glutathione transferases b. Sulfotransferases. -Describe the functions of: a. Glutathione transferases Describe the functions of

Production of healthcare - contestability and measurability, Normal 0 ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What is the basic structure of a virus, What is the basic structure of a vi...

What is the basic structure of a virus? Viruses are constituted of genetic material (DNA or RNA) covered by a protein capsule also called as a capsid. Some viruses, like HIV, h

What are protozoans? explain their functions, Protozoans are microscopic in...

Protozoans are microscopic in size however some are large enough to be seen with naked eye. Microscopic organisms such as these have numerous advantages, one of which is that they

What are some examples of organs and tissues, What are some examples of org...

What are some examples of organs and tissues where mitosis is more frequent, less frequent or practically absent? Generally in vertebrates mitosis is more frequent in a tissue

Transport in plants, Osmosis and transpiration both play a part in the move...

Osmosis and transpiration both play a part in the movement of water through a plant. Which of these two processes makes the greater contribution to the movement of water up the t

Countercurrent flow, Countercurrent flow is the arrangement by which fish ...

Countercurrent flow is the arrangement by which fish get oxygen from the water which flows by the help of their gills. The water flows across the respiratory surface of the gill i

Etiologic factor of congestive cardiac failure, Q. Etiologic factor of Cong...

Q. Etiologic factor of Congestive Cardiac Failure? Congestive heart failure develops over a period of time when the necrotic tissues are not replaced by functional connective c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd