Explain about the zone electrophoresis, Biology

Assignment Help:

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.


Related Discussions:- Explain about the zone electrophoresis

Organization levels of life, Q. Define the Organization Levels of Life? ...

Q. Define the Organization Levels of Life? Ans. The cell is the basic unit of life - the smallest unit that can carry on all the processes of life. Organisms that consist of a

Determine the benefits of exercises, Benefits of Exercises Benefits of ...

Benefits of Exercises Benefits of exercises for a diabetic patient are given below: - Improves sugar control. - Decreases HbA1C level. - Decreases blood pressure. -

Ethological isolation, Members belonging to different species refrain from ...

Members belonging to different species refrain from mating because of the behavioural differences between them. Such behavioural differences usually centre around specific courtshi

Classification of freshwater ecosystems, Classification of Freshwater Ecosy...

Classification of Freshwater Ecosystems Fresh water ecosystems depend on the terrestrial ecosystems for large quantities of organic and inorganic matter which are constantly a

Excretion, what is the composition of urine in fishes and reptiles ?

what is the composition of urine in fishes and reptiles ?

Elaborate the internal vascular circulation of an eye, Elaborate the intern...

Elaborate the internal vascular circulation of an eye. Retinal vessels are derived from central retinal artery and are distributed within the inner two-third of retina. Outer o

Taxonmy, classification on taxonomy

classification on taxonomy

Explain about modifiable risk factors, Q. Explain about Modifiable Risk Fac...

Q. Explain about Modifiable Risk Factors? 1. Behavioural Smoking Sedentary Lifestyle Habits Dietary Errors 2. Physiological Hyperlipidemia Hypertension Obe

Hydrogen-ion concentration, The acidity and alkalinity (pH) of an environme...

The acidity and alkalinity (pH) of an environment has a strong influence on the activity and stability of macromolecules such as enzymes. These enzymes play an important role durin

Hormones of pancreas, HORMONE S OF PANCREAS AND THEIR ROLE - (i) Glu...

HORMONE S OF PANCREAS AND THEIR ROLE - (i) Glucagon (Secreted by a-cells) It stimulates the liver to convert stored glycogen into glucose. Glucagon is controlled b

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd