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Explain about the Gel bands detection techniques?
Gel bands, resulting from a gel electrophoretic separation, may be detected by staining, radioactive counting or immunoblotting. Proteins in microgram quantities are often visualized by staining with Coomassie Brilliant Blue. Gel bands with lesser amount of protein can be visualized with silver stain which is 50 times more sensitive. Fluorescamine an alternative type of protein stain is a non- fluorescent molecule that reacts with primary amines, such as lysine, to yield an addition product that is highly fluorescent under UV irradiation. Proteins, DNA etc. can be detected through the UV absorption of a gel along its length.
If the sample is radioactive, the gel may dry under vacuum after wrapping in cellophane sheet and then damped over a sheet of X-ray film. After some time (few minutes to many weeks depending on the radiation intensity), the film is developed and the resulting autoradiograph shows the position of the radioactive components on 3. Enumerate the different types of electrophoresis.latter method yields quantitatively more accurate results than autoradiography. If an antibody to the protein of interest is available, it is possible to specifically detect this protein on the gel in the presence of many other proteins by immunoblot or western blot. With this brief review we move over to the last electrophoresis method i.e. disc electrophoresis.
Q. What are some representatives of the echinoderm phylum? Starfishes, sea urchins, sea cucumbers (holothurians) and brittle stars are examples of echinoderms.
You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate
Development of Ovule The ovule develops from a specialised region of the ovary - the placenta. Initially it appears as a small mound on the placenta, and is composed of homoge
1. In cattle, the polled condition (H) is dominant to the horned condition (h). A cross between an individual with red coat (R) and white coat (W) results in roan (RW). A polled, r
Candidiasis Candidiasis (Moniliasis) is a disease of man, animals and birds and is caused chiefly by Candida albicans. Crop mycosis (thrush) of poultry is caused by C. albicans
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Define Types of Indicators? Macro indicators are used at strategic levels while micro indicators are used at performance levels. From the previous sections it is clear that man
Define beneficial effects of functional foods? Several beneficial effects of functional foods have been reported, which include effects such as antioxidant, anticarcinogenic, b
Anaphase This phase is of shortest duration. It begins with a sudden separation of the two chromatids of each due to splitting of its centromere and then a slow mov
what are the general methods for studying microbial physiology
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