Explain about the gel bands detection techniques, Biology

Assignment Help:

Explain about the Gel bands detection techniques?

Gel bands, resulting from a gel electrophoretic separation, may be detected by staining, radioactive counting or immunoblotting. Proteins in microgram quantities are often visualized by staining with Coomassie Brilliant Blue. Gel bands with lesser amount of protein can be visualized with silver stain which is 50 times more sensitive. Fluorescamine an alternative type of protein stain is a non- fluorescent molecule that reacts with primary amines, such as lysine, to yield an addition product that is highly fluorescent under UV irradiation. Proteins, DNA etc. can be detected through the UV absorption of a gel along its length.

If the sample is radioactive, the gel may dry under vacuum after wrapping in cellophane sheet and then damped over a sheet of X-ray film. After some time (few minutes to many weeks depending on the radiation intensity), the film is developed and the resulting autoradiograph shows the position of the radioactive components on 3. Enumerate the different types of electrophoresis.latter method yields quantitatively more accurate results than autoradiography. If an antibody to the protein of interest is available, it is possible to specifically detect this protein on the gel in the presence of many other proteins by immunoblot or western blot. With this brief review we move over to the last electrophoresis method i.e. disc electrophoresis.


Related Discussions:- Explain about the gel bands detection techniques

Synapse, what is a synapse and what does it do?

what is a synapse and what does it do?

Mouth examination of new born, Mouth Inspect and palpate the lips, ...

Mouth Inspect and palpate the lips, gums, tongue, palate and oropharynx. Rule out cleft lip and palate, size of chin if small and receding it  shows micrognathia ind

What are important neurotransmitters, Q. What are some important neurotrans...

Q. What are some important neurotransmitters? The following are some neurotransmitters: serotonin, noradrenaline (norepinephrine), histamine,acetylcholine, adrenaline (epinephr

Do you know how ketoacidosis develops, Q. Do you know how ketoacidosis deve...

Q. Do you know how ketoacidosis develops? The chain of reaction begins with insufficient insulin to meet the needs of the body. Several factors then work together. These inclu

Distribution of stomata, Distribution of Stomata There are two paramet...

Distribution of Stomata There are two parameters normally used for expressing the distribution of stomata. Stomatal frequency: The number of stomata per unit area

What is the vector of chagas disease, Q. What is the vector of Chagas' dise...

Q. What is the vector of Chagas' disease? How is the disease transmitted? The vector of the Chagas' disease is its intermediate host, a triatomine bug. The key species is Triat

Digestive gland, which system give the signals to salivary gland

which system give the signals to salivary gland

How bone density affect osseointegration, How Bone density affect Osseointe...

How Bone density affect Osseointegration The most important bone property is density which is influenced by factors such as patient age and genetics. Higher density bones have

How different are the concepts of neoplasia-tumor and cancer, Q. How differ...

Q. How different are the concepts of neoplasia, tumor and cancer? Not all tumor is neoplastic and not every neoplasia creates tumor. The Tumor is the generic name given to the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd