Explain about the gel bands detection techniques, Biology

Assignment Help:

Explain about the Gel bands detection techniques?

Gel bands, resulting from a gel electrophoretic separation, may be detected by staining, radioactive counting or immunoblotting. Proteins in microgram quantities are often visualized by staining with Coomassie Brilliant Blue. Gel bands with lesser amount of protein can be visualized with silver stain which is 50 times more sensitive. Fluorescamine an alternative type of protein stain is a non- fluorescent molecule that reacts with primary amines, such as lysine, to yield an addition product that is highly fluorescent under UV irradiation. Proteins, DNA etc. can be detected through the UV absorption of a gel along its length.

If the sample is radioactive, the gel may dry under vacuum after wrapping in cellophane sheet and then damped over a sheet of X-ray film. After some time (few minutes to many weeks depending on the radiation intensity), the film is developed and the resulting autoradiograph shows the position of the radioactive components on 3. Enumerate the different types of electrophoresis.latter method yields quantitatively more accurate results than autoradiography. If an antibody to the protein of interest is available, it is possible to specifically detect this protein on the gel in the presence of many other proteins by immunoblot or western blot. With this brief review we move over to the last electrophoresis method i.e. disc electrophoresis.


Related Discussions:- Explain about the gel bands detection techniques

Illustrate the removal of carbon dioxide from the body, Which of the follow...

Which of the following processes in capillaries in the lung assist in the removal of carbon dioxide from the body? A. Net flux of carbon dioxide from red blood cells into plasm

Explain the phase contrast microscope, Explain the Phase Contrast Microscop...

Explain the Phase Contrast Microscop? Unpigmented and unstained living cells can be easily observed by phase contrast microscope. It has a special objective and a condenser th

Prove thet nucleic acid is the transforming agent, Fredrick Griffith publis...

Fredrick Griffith published a series of experiments that collectively were dubbed "The Transforming Principle" in 1928. Which of the following stopped him from being able to declar

Ecosystem and environment, How ecosyestem concept is useful to the study of...

How ecosyestem concept is useful to the study of environment?

What is the difference between nematocyst and spicules, What is the differe...

What is the difference between nematocyst and spicules? Nematocysts are on Cnidarians Spicules are in Poriferae

Describe the main cycle in metabolism of cornea, Describe the main cycle in...

Describe the main cycle in metabolism of cornea. Main Cycle: Glycolysis is represented by the central chain and involves the anaerobic conversion of glucose into pyruvic

Illustrate fluoxetine is an antidepressant drug or not, Fluoxetine is an an...

Fluoxetine is an antidepressant drug that presents an action mechanism related to the synaptic transmission. What is that mechanism? Fluoxetine is a substance that inhibits th

Emboly movement - involution, Emboly Movement - Involution It den...

Emboly Movement - Involution It denotes turning in or rolling over. Involution of mesodermal blastomeres has been observed in Amphioxus, reptiles, monotremes, amphibians,

What would be its final volume, Consider a simple spherical model cell that...

Consider a simple spherical model cell that consists of cytoplasm and a plasma membrane. The cell's initial volume is 2 nL and contains 0.2 M protein. The cell is placed in a la

Explain the urinary system in human body, Explain the Urinary System in hum...

Explain the Urinary System in human body? The urinary system removes the waste materials produced as a result of all the metabolic process required to sustain life. The process

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd