Explain about the gel bands detection techniques, Biology

Assignment Help:

Explain about the Gel bands detection techniques?

Gel bands, resulting from a gel electrophoretic separation, may be detected by staining, radioactive counting or immunoblotting. Proteins in microgram quantities are often visualized by staining with Coomassie Brilliant Blue. Gel bands with lesser amount of protein can be visualized with silver stain which is 50 times more sensitive. Fluorescamine an alternative type of protein stain is a non- fluorescent molecule that reacts with primary amines, such as lysine, to yield an addition product that is highly fluorescent under UV irradiation. Proteins, DNA etc. can be detected through the UV absorption of a gel along its length.

If the sample is radioactive, the gel may dry under vacuum after wrapping in cellophane sheet and then damped over a sheet of X-ray film. After some time (few minutes to many weeks depending on the radiation intensity), the film is developed and the resulting autoradiograph shows the position of the radioactive components on 3. Enumerate the different types of electrophoresis.latter method yields quantitatively more accurate results than autoradiography. If an antibody to the protein of interest is available, it is possible to specifically detect this protein on the gel in the presence of many other proteins by immunoblot or western blot. With this brief review we move over to the last electrophoresis method i.e. disc electrophoresis.


Related Discussions:- Explain about the gel bands detection techniques

Parasitic adoptaion in animal, #question.what do u mean by parasitic habit....

#question.what do u mean by parasitic habit.give account of various parasitic adoptation in animal.

Fever, what are the two benefits of fever with explaination??

what are the two benefits of fever with explaination??

Explain water-soluble and fat-soluble vitamins, What is the difference betw...

What is the difference between water-soluble and fat-soluble vitamins? Why can fat-soluble vitamins cause harm when ingested in excess? Water-soluble vitamins are those soluble

How many cellular nuclei does the pollen tube have, How many cellular nucle...

How many cellular nuclei does the pollen tube of angiosperms have? What is the ploidy of each of these nuclei? The pollen tube that is the mature male gametophyte of angiosperm

Outbreak of staphylococcal food poisoning, Q. What are the conditions favou...

Q. What are the conditions favouring the outbreak of Staphylococcal food poisoning? • Botulism is caused by the bacteria Clostridium botulinum. • All low acid foods can s

Risk factor modification, Risk Factor Modification :  Even after CABG,...

Risk Factor Modification :  Even after CABG, patients are at risk of progression of native coronary artery disease and development of lesions in the conduits. Modification of

Define biocompatible and biodegradable material, 1. Define each term and gi...

1. Define each term and give an example of an application that uses the following: a. Biocompatible material b. Biodegradable material c. Biomimetic

Main parts into which a neuron can be divided, Q. What are the three main p...

Q. What are the three main parts into which a neuron can be divided? What are their respective functions? The three mains parts into which a neuron can be didactically divided

Assessment of diastolic function, Diastolic dysfunction is  responsible for...

Diastolic dysfunction is  responsible for one third of cases of heart  failure alone and rest two  third of cases in  combination with systolic dysfunction. Diastolic dysfunction c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd