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Explain about the Gel bands detection techniques?
Gel bands, resulting from a gel electrophoretic separation, may be detected by staining, radioactive counting or immunoblotting. Proteins in microgram quantities are often visualized by staining with Coomassie Brilliant Blue. Gel bands with lesser amount of protein can be visualized with silver stain which is 50 times more sensitive. Fluorescamine an alternative type of protein stain is a non- fluorescent molecule that reacts with primary amines, such as lysine, to yield an addition product that is highly fluorescent under UV irradiation. Proteins, DNA etc. can be detected through the UV absorption of a gel along its length.
If the sample is radioactive, the gel may dry under vacuum after wrapping in cellophane sheet and then damped over a sheet of X-ray film. After some time (few minutes to many weeks depending on the radiation intensity), the film is developed and the resulting autoradiograph shows the position of the radioactive components on 3. Enumerate the different types of electrophoresis.latter method yields quantitatively more accurate results than autoradiography. If an antibody to the protein of interest is available, it is possible to specifically detect this protein on the gel in the presence of many other proteins by immunoblot or western blot. With this brief review we move over to the last electrophoresis method i.e. disc electrophoresis.
describe polymorphism in lysosomes in short
An A=T mispairing leads to an A=C substitution. The other DNA helix will contain a(n) __ pair. a. A=C b. A=T c. G=C d. B=Q e. T=T Can you also explain it please so if I'm ask
During mitotic anaphase is there separation of homologous chromosomes or separation of identical chromatids? In the anaphase of mitosis the identical chromatids separate and co
Define Consistency and Texture - Dysphilgia? Aspiration of liquids, including water, into the lungs can result in complications. To avoid this problem and yet fulfilling the fl
nutrition requirement for protoa
PURPOSES OF PATIENT's /CLIENT's RECORDS Following purposes are served by maintaining patient's/client's records: A means of communication. A basis on which therap
Limitations of Computed Tomograpy Scan This technology is costly Increased amount for radiation
The concentration of the tissue fluid, which bathes all cells in the body, is kept more or less constant. Why is this important? If the tissue fluid became more dilute, the ce
Define Proline It is a prominent amino acid found in fish and may contribute to sweetness. The sugars ribose, glucose and glucose-6-phosphate are flavour contributors, as is 5-
What is Physical activity ratio? Physical activity ratio (PAR): The energy cost of an activity per unit of time (usually a minute or an hour) expressed as a multiple of BMR. It
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