Explain about the gel bands detection techniques, Biology

Assignment Help:

Explain about the Gel bands detection techniques?

Gel bands, resulting from a gel electrophoretic separation, may be detected by staining, radioactive counting or immunoblotting. Proteins in microgram quantities are often visualized by staining with Coomassie Brilliant Blue. Gel bands with lesser amount of protein can be visualized with silver stain which is 50 times more sensitive. Fluorescamine an alternative type of protein stain is a non- fluorescent molecule that reacts with primary amines, such as lysine, to yield an addition product that is highly fluorescent under UV irradiation. Proteins, DNA etc. can be detected through the UV absorption of a gel along its length.

If the sample is radioactive, the gel may dry under vacuum after wrapping in cellophane sheet and then damped over a sheet of X-ray film. After some time (few minutes to many weeks depending on the radiation intensity), the film is developed and the resulting autoradiograph shows the position of the radioactive components on 3. Enumerate the different types of electrophoresis.latter method yields quantitatively more accurate results than autoradiography. If an antibody to the protein of interest is available, it is possible to specifically detect this protein on the gel in the presence of many other proteins by immunoblot or western blot. With this brief review we move over to the last electrophoresis method i.e. disc electrophoresis.


Related Discussions:- Explain about the gel bands detection techniques

Define supplementary feeding to control under nutrition, Define Supplementa...

Define Supplementary feeding to control under nutrition? Supplementary feeding has remained an important component to control under nutrition. Considering the dietary inadequac

Importance of fat synthesis and storage, Importance  of fat  synthesis  ...

Importance  of fat  synthesis  and storage : As  is clear from above  account, all  surplus nutrients, digested and  absorbed  from above the  immediate need of  body, are readily

TISSUES, HOW TO WRITE A CONCLUSION ON A ASSIGNMENT OF EPITHELIAL TISSUES

HOW TO WRITE A CONCLUSION ON A ASSIGNMENT OF EPITHELIAL TISSUES

Four distinct phases of demographic transition, Four Distinct Phases of Dem...

Four Distinct Phases of Demographic Transition Demographic transition takes place in four distinct phases. 1. Re-industrial stage in which harsh living conditions lead to

How can the presence, Q. How can the presence, function of muscular tissue ...

Q. How can the presence, function of muscular tissue and localization in beings of the phylum Annelida is explained? In these beings there are a longitudinal muscular layer und

Neurulation in amphibians, Neurulation in Amphibians The first step i...

Neurulation in Amphibians The first step in the neurulation process is the flattening and thickening of dorsal ectoderm to form neural plate. The plate of cells are different

Fluids requirement during congestive cardiac failure, Q. Fluids requirement...

Q. Fluids requirement during congestive cardiac failure? Fluids: Fluid intake should be monitored in accordance with urine output and severity of oedema. Fluid restriction is

What is the results of congenital pulmonary stenosis, What is the Results o...

What is the Results of Congenital Pulmonary Stenosis? Early mortality for open pulmonary valvotomy in neonates varies between 6 and 10 per cent. For infants and children, surgi

State the success which signifies optimum health, Success which signifies O...

Success which signifies Optimum Health The features or clinical conditions which are representative of this group are: i) No pain or tenderness in the implant upon function.

What do you mean by genera, Q. What do you mean by Genera? Like species...

Q. What do you mean by Genera? Like species the genus represents a concept. Genera (Plural) are aggregates of closely related species. There is no size requirement for a genus.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd