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Explain about the Gel bands detection techniques?
Gel bands, resulting from a gel electrophoretic separation, may be detected by staining, radioactive counting or immunoblotting. Proteins in microgram quantities are often visualized by staining with Coomassie Brilliant Blue. Gel bands with lesser amount of protein can be visualized with silver stain which is 50 times more sensitive. Fluorescamine an alternative type of protein stain is a non- fluorescent molecule that reacts with primary amines, such as lysine, to yield an addition product that is highly fluorescent under UV irradiation. Proteins, DNA etc. can be detected through the UV absorption of a gel along its length.
If the sample is radioactive, the gel may dry under vacuum after wrapping in cellophane sheet and then damped over a sheet of X-ray film. After some time (few minutes to many weeks depending on the radiation intensity), the film is developed and the resulting autoradiograph shows the position of the radioactive components on 3. Enumerate the different types of electrophoresis.latter method yields quantitatively more accurate results than autoradiography. If an antibody to the protein of interest is available, it is possible to specifically detect this protein on the gel in the presence of many other proteins by immunoblot or western blot. With this brief review we move over to the last electrophoresis method i.e. disc electrophoresis.
List three important discoveries that resulted from the Human Genome Project. Answers should contain three of the following: Only about 2 percent of the human genome encodes p
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Why would the O-H bond in C=O-H and the OH bonding with a water molecule be considered weak?
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What is the quaternary structure of a protein? Do all proteins have quaternary structure? The quaternary protein structure is the spatial conformation because of interactions b
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