Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

What is the properties of aqueous solutions, Which of the following is TRUE...

Which of the following is TRUE about the properties of aqueous solutions? Select one: a. A pH change from 5.0 to 6.0 reflects an increase in the hydroxide ion concentration (

Explain causes of shigellosis, Q. Explain causes of Shigellosis? Shigel...

Q. Explain causes of Shigellosis? Shigellosis is caused by the genus Shigella and is a common enteric (intestinal) infection. The genus has four serological groups,

O-linked oligosaccharides , Most proteins made by ribosomes on the RER (rou...

Most proteins made by ribosomes on the RER (rough endoplasmic reticulum) are glycoproteins, which is they hold short chains of carbohydrates (oligosaccharides) covalently related t

Define methods for estimation of iron and haemoglobin, Define Methods for E...

Define Methods for Estimation of Iron and Haemoglobin? In this section we shall study the procedure involved in estimation of iron in any sample first followed by a review on t

Rehabilitation phase for nutritional treatment -neuro trauma, Rehabilitatio...

Rehabilitation Phase for nutritional treatment - Neuro Trauma? Once the patient stabilizes and starts recovering, be sides nutritional replacement, there is a need to assess fu

Myelinated fibre, Myelinated fibre Axon is a relatively long process (...

Myelinated fibre Axon is a relatively long process (also quite often referred to as nerve fibre) and can be considered as functionally specialised for conduction of excitation

Define meat as a rich source of protein, Define Meat as a Rich Source of Pr...

Define Meat as a Rich Source of Protein? Skeletal or striated muscles are used for food purposes. Flesh of cattle, sheep and swine comprise most of the meat contents. Edible me

Explain the modes of ventilation, Explain the Modes of Ventilation? In ...

Explain the Modes of Ventilation? In general, mechanical ventilation can be done to deliver a set tidal volume (volume controlled) or a set pressure (pressure controlled). Some

What is the meaning of trocophore, What is the meaning of Trocophore? T...

What is the meaning of Trocophore? This free-swimming ciliated larval stage is found in numerous animal phyla including Mollusca and Annelida. Larvae has a unique circle of pre

What are the antigens and antibodies of abo blood system, What are the anti...

What are the antigens and antibodies of each blood type of the ABO blood system? Type A: antigen A, antibody anti-B. Type B: antigen B, antibody anti-A. Type AB: antigens A and

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd