Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

Deficiency diseases-thiamine deficiency (hypothiaminosis), Thiamine deficie...

Thiamine deficiency (hypothiaminosis) Thiamine deficiency is characterized by signs of nervous system. The primary  thiamine deficiency is not common in animals. However, seco

Epiboly of ectoderm, Epiboly of Ectoderm Like the presumptive endoder...

Epiboly of Ectoderm Like the presumptive endodermal notochordal and mesodermal cells migrate inward the areas in the epiblast vacated through them are occupied by expansion (

Properties of pyridoxine, Properties of pyridoxine a)  It forms white, ...

Properties of pyridoxine a)  It forms white, odourless  crystals. b)  The compound is readily soluble  in water. c)  When  a neutral or alkaline solution of pyridoxine is

Volvox, what is the necessity of interdependence of a volvox organism in th...

what is the necessity of interdependence of a volvox organism in the colonial existence?

Which part of the skin is the pigment injected, Permanent tattoos are made ...

Permanent tattoos are made by injecting pigment into the skin. Into which part of the skin is the pigment injected, the epidermis or dermis?

Functions of testis, Functions of Testis The testis performs the follo...

Functions of Testis The testis performs the following two functions: Proliferation of spermatozoa and Secretion of steroid hormones

Explain wire loop and tube method, Explain Wire loop and Tube Method 1....

Explain Wire loop and Tube Method 1.  Use a 25-gauge dental injection needle, cut to remove beveled end 2. Both ends of-mm diameter steel ligature wire passed through the ne

Surgical treatment options, Cardiomyoplasty This is a procedure wherei...

Cardiomyoplasty This is a procedure wherein the latissimus dorsi muscle is wrapped around the heart and stimulated to contract synchronously with it. It has been largely given

Explain nutritional management for neuro trauma patients, Define Feeding an...

Define Feeding and Nutritional Management for Neuro trauma? The main objective of nutritional management is to counteract the hypermetabolism associated with inflammation. The

What is sex-influenced dominance, What is sex-influenced dominance? The...

What is sex-influenced dominance? The Sex-influenced dominance is the phenomenon in which the manifestation of a phenotype of a gene in heterozygosity depends on the sex of the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd