Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

How much template dna will you add, You are setting up a PCR reaction. a) T...

You are setting up a PCR reaction. a) The total volume of your reaction is 50 uL. Your stock primer solutions are 25 mM each. You want a final concentration of primer of 1 mM in yo

Which are mineral salts, Q. Which are mineral salts? Where in living beings...

Q. Which are mineral salts? Where in living beings can found mineral salts? Mineral salts are simple inorganic substances made of metallic chemical elements, like sodium, iron,

Which is right of the resting membrane potential, Which is true of the rest...

Which is true of the resting membrane potential? A. It requires few ions to be distributed unevenly. B. It has the same value in all cells C. Only nerve and muscle cells h

Explain Stirred-tank bioreactors, Normal 0 false false fals...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What would most likely be true, You are given a metaphase chromosome prepar...

You are given a metaphase chromosome preparation (a slide) from an unknown organism that contains 12 chromosomes. Two that are clearly smaller than the rest appear identical in len

Explain the process of diagnosis of cholera, Explain the process of diagnos...

Explain the process of diagnosis of cholera Diagnosis: Cholera can be confirmed only by the isolation of the causative organism from the diarrheic stools of infected individual

Slow walking or crawling, Slow walking or Crawling This type of locom...

Slow walking or Crawling This type of locomotion is seen while the animal moves on the substratum. It involves a metachronal rhythm of action in the parapodia. Each fifth or

How many distinct hexapeptides made from 20 amino acids, How many distinct ...

How many distinct hexapeptides can be made from 20 amino acids?

Explain etiology and clinical feature of parkinson''s disease, Explain the ...

Explain the Etiology and Clinical Features of Parkinson's disease? The cause of Parkinson's is unknown. Genetic predisposition (in most cases the reason for the death of these

Of which substance are microtubules made, Of which substance are microtubul...

Of which substance are microtubules made? In which structures and cellular processes do microtubules participate? Microtubules are made of consecutive dimers of the protein tub

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd