Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

Temperate deciduous forest - ecosystem, Temperate deciduous forest - Ecosys...

Temperate deciduous forest - Ecosystem The temperate forests are characterised by a moderate climate and broad-leafed deciduous trees, which shed their leaves in fall, are bar

Light and dark reactions, Evidence for Existence of Light and Dark Reaction...

Evidence for Existence of Light and Dark Reactions The process of photosynthesis was known in its bare outline already at the beginning of this century. But the phenomenon wa

Define procedure for determination of fungal and yeast count, Define Proced...

Define Procedure for Determination of Fungal and Yeast Count? Now carry out the exercise following the steps enumerated herewith: 1. Shake the food sample and aseptically tr

Define growth hormone releasing hormone receptors, Define GHRH Receptors (G...

Define GHRH Receptors (Growth Hormone Releasing Hormone Receptors)? A. GHRH Receptors are only located in the plasma membranes of cells in the hypothalamus. B. GHRH Receptor

Horse diseases-equine encephalomyelitis, Equine encephalomyelitis Encep...

Equine encephalomyelitis Encephalomyelitis or inflammation of the brain tissue and the spinal cord, can be brought about by different viruses. Eastern-, Western- and Venezuelan

Fuel requirements - deforestation, Fuel Requirements - Deforestation T...

Fuel Requirements - Deforestation The increasing demand for fuel wood is one of the major factors leading to the degradation of the forest ecosystem. Fuel wood is of such majo

Is rabies caused by virus or by bacteria?, Q. Is rabies caused by virus or ...

Q. Is rabies caused by virus or by bacteria? How is it transmitted? Which organs and tissues are affected by the disease? How is it prevented and treated? The Rabies, as well k

Agro industrial-calcium, Calcium   The calcium content in forages is affe...

Calcium   The calcium content in forages is affected by species, plant part (leaf versus stem), plant maturity, soil content of calcium and climate. Forages are usually a good so

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd