Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

The best describes the acid properties of guanine, Which statement best des...

Which statement best describes the acid/base properties of guanine? -at pH, guanine will be negatively charge -at pH, guanine will be neutral -it will be an effective buff

Pre-embryonic development, Pre-Embryonic Development We had said in t...

Pre-Embryonic Development We had said in the starting of the unit that prenatal development can be divided into two phases, embryonic and foetal. The embryonic phase of devel

State the posterior superior alveolar nerve, Posterior superior alveolar ne...

Posterior superior alveolar nerve and vessels It is found in the  posterior aspect of the maxilla running between the bone and the lining of the maxillary sinus may be injured

Define nutritional management of neurological disorders, Define Nutritional...

Define Nutritional Management of Neurological Disorders Your friend's aged father, over a period of time has become very confused, forgetful and is unable to do many things he

Microtubules, MICROTUBULES Discovered by De Robertis and Franchi ...

MICROTUBULES Discovered by De Robertis and Franchi . Term given by Slautterback . These are hollow structures, consists of tubulin protein. Each protein diamer

Blotting technique to detect particular specific proteins, Blotting method ...

Blotting method to detect particular one proteins Ans) Western blotting

Explain selective and differential culture media, Explain Selective and Dif...

Explain Selective and Differential Culture Media? These are used for isolation of specific organisms. These are designed to isolate a group of closely related organisms and dif

Explain about harvesting autogenous bone grafts, What are the precautions t...

What are the precautions to be taken when harvesting autogenous bone grafts from mandibular symphysis and ramus? When harvesting grafts from the mandibular symphysis region, fo

Explain nephrotic syndrome, Explain Nephrotic syndrome Nephrotic syndr...

Explain Nephrotic syndrome Nephrotic syndrome :- Kidney disease due to degeneration of renal tubule.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd