Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

List host factor which play important role in implant, List host factor whi...

List host factor which play important role in implant survival. Host factors: a) Medical status of the patient b) Habits - Smoking - Parafunctional habits c) O

Growth curve, typical growth curve in plants

typical growth curve in plants

Photosynthesis, Why methyl alcohol is used in destarching?

Why methyl alcohol is used in destarching?

Determine the nature and degree of abnormality, Determine the Nature and De...

Determine the Nature and Degree of Abnormality The nature and degree of abnormality will effect normal neural growth and maturational outcome. For example, in an adult damage t

Which glands secrete their product into circulatory system, Name of the duc...

Name of the ductless glands that secrete their product into the circulatory system are: a) Exocrine (pron: ek-seh-kren) b) Apocrine (pron: ap-eh-kren) c) Holocrine (pron:

Digestive system - pharynx, PHARYN X - Commom system between digestive...

PHARYN X - Commom system between digestive system and respiratory system. In it gullet and glottis present.Gullet leads to oesophagus. Glottis lead to trachea. Wall of p

What is hypercholeslerolaemia, Q. What is hypercholeslerolaemia? Choles...

Q. What is hypercholeslerolaemia? Cholesterol: It is a natural component of foods such as mutton, pork, ham, sausages, lamb, chicken, eggs (yellow), whole milk, cheese, ice-cre

Explain echinocandins, Explain Echinocandins It inhibit synthesis of β ...

Explain Echinocandins It inhibit synthesis of β (1,3)-D-glucan, an essential component of the fungal cell wall. The potential for adverse effects in humans is low because of th

Explain that a peptide bond a covalent, Is a peptide bond a covalent or a n...

Is a peptide bond a covalent or a non-covalent bond? Please explain.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd