Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

Explain the properties of gum tragacanth, Explain the Properties of Gum tra...

Explain the Properties of Gum tragacanth Gum tragacanth swells in water to give thick  viscosity dispersions or pastes similar in texture to soft gels. The viscosity shows a sh

Define barrier layer cells (photocells), Define Barrier layer cells (photoc...

Define Barrier layer cells (photocells) They are the very simple of the detectors. It contains the bottom support layer of conductive metal like iron. A photosensitive layer of

Illustrate disorders of the gastrointestinal tract, Q. Illustrate Disorders...

Q. Illustrate Disorders of the gastrointestinal tract? A host of disorders associated with a part or entire of the gastrointestinal tract. The important and the most frequent o

Determine the two different alleles, What is meant by saying that in relati...

What is meant by saying that in relation to a given trait conditioned by a gene with two different alleles the gametes are always "pure"? To say that gametes are pure means tha

Define endocrine systems and metabolism, Define Endocrine Systems and Metab...

Define Endocrine Systems and Metabolism? As mentioned earlier, the main endocrine gland-pituitary gland secretion decreases. Another important feature in elderly is decline in

What action is epa taking on plant-incorporated protectants, What action is...

What action is EPA taking on plant-incorporated protectants? EPA has issued three final rules clarifying and formalizing EPA's framework for federal oversight of plant-incorpor

Explain adverse effects of ketoconazole, Explain Adverse Effects of ketocon...

Explain Adverse Effects of ketoconazole Anorexia, nausea and vomiting are common with higher doses (>400 mg/day) of ketoconazole; taking the drug with food or at bedtime may i

How are living beings divided into two groups, According to cellular organi...

According to cellular organization how are living beings divided into two groups? Cellular beings are separated into two groups: the prokaryotes, unicellular beings whose sole

Define the left cerebral cortex, Which of the following is true for a prima...

Which of the following is true for a primary motor cortex (M1) corticospinal interneuron that produces action potentials during movements of the big toe of the right foot? A. A

Describe about psycho-social change in children, Describe about Psycho-soci...

Describe about Psycho-social Change in children? Adolescence is a period of maturation for both mind and body. Along with the physic4 growth, emotional and intellectual develop

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd