Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

Why could a disease be more serious in a population, Why could a disease be...

Why could a disease be more serious in a population with a low genetic diversity? A population with a low genetic diversity could be more expected to be wiped out by a disease

Proteins of animal origin - milk, Proteins of Animal Origin - Milk: Mi...

Proteins of Animal Origin - Milk: Milk: A value of 3.5% protein is often considered as an average for milk. Milk protein has traditionally been divided into 2 classes - casei

Name the organism included in symbiotic nitrogen fixation, Name the organis...

Name the organism included in symbiotic nitrogen fixation. What are the components needed for this pure? Determine their role. Differentiate among inbreeding and heterosis. The

Kuch, how vagina is inserted in cock

how vagina is inserted in cock

What are the near point and the far point of the vision, Q. What are the ne...

Q. What are the near point and the far point of the vision? The near point is the closest distance between the eye and an object that makes possible the formed image to be focu

Pancreatic juice resume the digestion of carbohydrates, Q. How does the pan...

Q. How does the pancreatic juice resume the digestion of carbohydrates? What is the involved enzyme? Carbohydrate digestion begins with the action of the salivary amylase (ptya

Conjugation - patterns of sexual reproduction, Conjugation - Patterns of Se...

Conjugation - Patterns of Sexual Reproduction Conjugation is a temporary union among two individuals of the same species, during which the original macronucleus disintegrates,

Define the spray drying method, Define the Spray Drying Method? Spray d...

Define the Spray Drying Method? Spray drying is a unique drying process since it involves both particle formation and drying. It is most suitable for drying of liquid foods suc

Kingdoms , phlum,class,characteristics of chromalveolata

phlum,class,characteristics of chromalveolata

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd