Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

What is the food processing, What is the Food Processing? Food processi...

What is the Food Processing? Food processing, as you learnt earlier, involves the conversion of raw materials and ingredients into an acceptable food product for the consumer.

Define effect on oestrogens and progesterone in pregnancy, Define effect on...

Define effect on Oestrogens and progesterone in pregnancy? Oestrogens and progesterone are secreted by the placenta from 8-10 weeks of gestation. Progesterone increases through

What do you understand by herbarium ethics, Q. What do you understand by He...

Q. What do you understand by Herbarium ethics? You should also know that herbaria provide us with policy statements including such information as 1) filing arrangements, 2) res

What are coacervates, What are coacervates what property do they have that ...

What are coacervates what property do they have that suggests they could be a primitive cell.

Collagen helix protein structure, Collagen Helix Collagen has a larg...

Collagen Helix Collagen has a large amount of glycine (25%) and proline (and hydroxyproline, 25%). They run parallel, form a right handed super-helix that is stabilized b

Explain about the micronutrient deficiencies, Explain about the Micronutrie...

Explain about the Micronutrient Deficiencies? Micronutrient deficiencies are more common during calamities. Can you guess, why? This is due to the lack of diversified food item

What are heterochromatin and euchromatin, Chromatin is uncondensed nuclear ...

Chromatin is uncondensed nuclear DNA, the typical DNA morphology in interphase (the phase of the cell cycle in which the cells is not separating itself). In this phase of the cell

Pre-embryo - human development, Pre-Embryo - Human Development About t...

Pre-Embryo - Human Development About thirty hours after fertilization the zygote goes through mitosis giving rise to 2 blastomeres. These blastomeres go through further cleava

Hematology case study , would i be able to get help on these case study que...

would i be able to get help on these case study questions

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd