Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

Nuclear membrane - structure of nucleus, KARYOTHEC A OR NUCLEAR MEMBRANE ...

KARYOTHEC A OR NUCLEAR MEMBRANE It is also called Nucleolemma, term given by Erclab & Hertwig. Karyotheca composed of two unit membranes separated by a 150-300 A° wide p

Define procedure for determination of fungal and yeast count, Define Proced...

Define Procedure for Determination of Fungal and Yeast Count? Now carry out the exercise following the steps enumerated herewith: 1. Shake the food sample and aseptically tr

Explain energetic function of organic molecules, What are some examples of ...

What are some examples of the energetic function of organic molecules? As they are complex molecules, presenting lots of chemical bonds, organic molecules keeps large amount of

What ways farmers try to improve the quality of their soil, a) In what way...

a) In what ways do farmers try to improve the quality of (i) their soil, (ii) their crop plants?    b) What other steps do farmers take to maximise the yield from their

Human impact on enviroment, What are the principal sources of of excessive ...

What are the principal sources of of excessive nitrate and phosphatebin rivers and lakes?

Explain global burden in the history of mart disease, Explain Global burden...

Explain Global burden in the history of mart disease? In 2001, 17 million people died of CVD of all types. The most important causes were ischemic heart disease (IHD), Hyperten

Man-made disasters- nuclear disasters, Among all the environmental disaster...

Among all the environmental disasters that might be caused by humans, nuclear disasters have the greatest potential of causing severe damage to life property. The released as a con

Fundamental aspects of protein structure, Can someone describe the fundamen...

Can someone describe the fundamental aspects of protein structure? I'm already familiar with the amino acid structure, I just don't have a clear conceptual understanding of protein

What are the main purpose of changes in life style, What are The main purp...

What are The main purpose  of changes in life style The main purpose  of changes in life style are to: - control blood sugar. - maintain the body weight of the patients.

Calculate how much of the absorbance of the bsa, For a sample containing bo...

For a sample containing both protein and nucleic acid, the absorbance measured at a particular wavelenght will be the sum of contributions from both types of molecule. using the pr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd