Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

State the uni-ocular movements, State the Uni-ocular Movements In each...

State the Uni-ocular Movements In each eye, for every movement there is an agonist, antagonist, and a synergist. An agonist is the main muscle that is active in carrying out t

Devices for controlling of particulate emissions, Particulate matter in gas...

Particulate matter in gaseous effluents consists of particles of size ranging from 0.1 um to 100 um. The device selection is based upon physical and chemical characteristic of part

Centrifugal pump-type of blood pump, Centrifugal Pump: This is available ...

Centrifugal Pump: This is available for clinical perfusion from 1976 (Bio Medicus Pump). It is disposable, causes less blood trauma and reduces the risk of massive air embolism.

Define prevention of idd - communication campaign, Define prevention of IDD...

Define prevention of IDD - Communication Campaign? A mass communication campaign is needed to create awareness in the community about the consequences of IDD and the benefits o

Currents in estuaries, Currents in Estuaries Currents in estuaries are...

Currents in Estuaries Currents in estuaries are caused primarily by tidal action and river flow. Currents are generally confined to channels but velocities up to several knots

Explain the main function of the digestive system, Explain the main  funct...

Explain the main  function of the digestive system The major function of the digestive system is to ingest the food materials, digest it to absorbable end products, absorb the

List the four objectives of counselling a diabetic patient, Q. List the fou...

Q. List the four objectives of counselling a diabetic patient? Objectives of Diabetes Counselling 1. Facilitating decision to start treatment and change life style 2.

Why the enzymatic action is highly specific, Why can it be said that the en...

Why can it be said that the enzymatic action is highly specific? The enzymatic action is highly specific because only exact substrates of one enzyme bind to the activation cent

Explain the the use of soy protein in food industry, The use of soy protein...

The use of soy protein concentrates in different applications in food industry is highlighted herewith. a. Bakery products Unlesss higher protein fortification leve

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd