Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

Define working of kidney in excretion of zinc in humans, Define working of ...

Define working of Kidney in excretion of zinc in humans? Very small amount of zinc is excreted in the urine (0.3-0.7 mg/day), as most of the zinc filtered by the kidney is reab

Chemical reactions which are catalysed by enzymes, Give two examples of che...

Give two examples of chemical reactions which are catalysed by enzymes in the course of brewing.  In the course of brewing, enzymes in the grain catalyse the conversion of star

Explain phenotypical characteristics of alleles of a gene, What are few exa...

What are few examples of phenotypical characteristics that present two or more varieties and of phenotypical features that do not vary? In relation to the genes correspondent to th

Nervous system - spinal nerves, SPINAL NERVES 31 Pairs. Mixed type. ...

SPINAL NERVES 31 Pairs. Mixed type. Total weight 150 gms. At the base of origin of spinal nerves gland of swammardon or calcarious ganglion is present. Composed of m

Conventions for using binomial nomenclature, Conventions for using binomial...

Conventions for using binomial nomenclature The names of higher categories are often synthesized from Latin words in the same manner as generic or specific names, and always s

Major divisions of the chordate phylum, Q. What are the two major divisions...

Q. What are the two major divisions of the chordate phylum? Chordates are divided into vertebrates and protochordates (urochordates and cephalochordates). Q. What are the d

Explain the food preparation and cooling - canning, Explain the Food prepar...

Explain the Food preparation and Cooling - Canning? Food preparation - Preparation methods will change with the type of food. For fruit, cutting to size, washing, grading, peel

What is centrioles in cell structure, What is Centrioles in cell structure?...

What is Centrioles in cell structure? Most cells of higher organisms contain pairs of cylindrical bodies called centrioles, which help to organize microtubules in cell division

List the stages of process of behaviour change, Q. List the stages of proce...

Q. List the stages of process of behaviour change? Stages of behaviour change process: · Unawareness · Awareness · Acquiring knowledge · Motivation · Trial

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd