Distribution coefficient -terminology used in chromatography, Biology

Assignment Help:

Distribution Coefficient - Terminologies used in Chromatography?

During the purification or separation of the biomolecules it should be kept in mind that two important factors influence the resolution (separation) of a mixture of compounds. These are effective distribution coefficient (i.e. the ratio of the amount present in one phase divided by the amount present in the other phase) and the length of the column. Thus, the basis for all forms of chromatography is the partition or distribution coefficient, wherein a compound distributes itself between two immiscible phases. For a compound distributing itself between equal volumes of two immiscible solvents A and B, the value of this coefficient is constant at a given temperature. The distribution coefficient can thus be described as:

Distribution coefficient/partition coefficient = Concentration in solvent A/ Concentration in solvent B


Related Discussions:- Distribution coefficient -terminology used in chromatography

What is the name of the larva of corals and sea anemones, What is the name ...

What is the name of the larva of corals and sea anemones? What is the biological significance of that larval stage? Sexual reproduction in corals and sea anemones has a larval

Causes of gastro oesophageal reflux disease, Q. Causes of gastro oesophagea...

Q. Causes of gastro oesophageal reflux disease? GERD may develop due to any of the following reasons: • decreased muscle tone or abnormal relaxation of the LES, • reduced st

What is the single tooth implant case, In Single Tooth Implant Case: a)...

In Single Tooth Implant Case: a) Vertical distance available between the crest of residual ridge to the opposing occlusal surface. At least 7 mm space is required to a cementab

Define factor contribute to malabsorption - radiation damage, Define Factor...

Define Factors Contribute to the Malabsorption - Radiation Damage? Malabsorption of water, fats, bile salts, carbohydrates, calcium, magnesium, iron and vitamin B 12 occur dur

Loss-of-function mutations, Choose the correct answer. A. The phenotype det...

Choose the correct answer. A. The phenotype determines the genotype. B. True-breeding individuals are produced by repeated backcrossing. C. Recessive alleles are usually loss-of-fu

Function of adenosine in consciousness, Q. Function of Adenosine in conscio...

Q. Function of Adenosine in consciousness? The adenosine plays a major role in inducing sleep. Injections of adenosine promote sleep and decrease wakefulness. Conversely, adeno

What property of water helps, South Dakota and Oregon are at similar latitu...

South Dakota and Oregon are at similar latitudes, and yet, on average, Oregon has warmer winters and cooler summers than South Dakota does. What property of water helps account for

What is the difference between smallpox and measles, Q. What is the differe...

Q. What is the difference between smallpox (variola) and measles? The Smallpox is a viral infection like measles. The Smallpox is transmitted by respiratory secretions, saliva

Biological significance of incompatibility, Biological Significance of Inco...

Biological Significance of Incompatibility Both interspecific and intraspecific incompatibility determines the degree of inbreeding and out breeding of plants. Self-incompati

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd