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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Define Fish as a rich source of protein? The edible portion is skeletal muscles of the body. Even though the skeletal muscles of different animals are basically similar, fish s
Determine the Occurrence of Vitamin E The tocopherols are widely found in animal and vegetable materials, like nuts and seeds. Considerable amounts are found in a number of
How many of the same proteins are made at the same time by each ribosome in the translation of one mRNA molecule? How does consecutive protein production occur in translation?
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Blotting method to detect particular one proteins Ans) Western blotting
explain industrial melanism and genetic repatterning during isolation with example
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Q. Besides the liver which is the other adnexal gland of the digestive system that releases substances in the duodenum participating in extracellular digestion? The other adnex
Define species according to the biological species concept. What two problems often make this definition impractical?
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