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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
LAMPBRUS H CHROMOSOMES Discovered by Ruckert . Lampbrush Chromosomes were seen in Oocytes of several different animals like shark, amphibians, reptiles and birds. (Most
Age-Sex Distribution While studying exponential growth we need to know more than just the birth and death rates because these rates vary with age and sex. Age structure in a
A number of protein-coding genes are active in all cells and are required for so- called house-keeping functions such as the enzymes of glycolysis, the citric acid cycle and the pr
Define Hydroxybenzoic acid and Hydroxycinnamic acid? Hydroxybenzoic acid: e.g. ellagic and gallic acids, which are hydrolyzable tannins, present in berries and nuts. Hydroxy
Giant Nerve Fibres We now know that giant nerve fibres take place in the central nervous system of many polychaetes, oligochaetes, insects cephalopod etc. They are broader an
Describe the Plasma Homocysteine levels ? Several studies have investigated the contribution of homocysteine to CVD risk both among immigrant Indians and those living in India.
Q. What are the pentoses? What are the vital roles of pentoses in DNA and RNA molecules? Pentoses are carbohydrates whole of five carbons. The DNA molecule is made of a sequ
If a large volume of fluid has shifted from the blood into the peritoneal cavity, how would this affect blood volume and hematocrit?
FUNCTIONS OF SKIN - Skin performs various diverse functions, that is why it is called "jack of all trades". 1 . Protection - The skin protects the internal soft or
Salient Features of pulmonary tuberculosis The salient features tuberculosis include: Wasting of tissues Exhaustion Cough Expectoration, and Fever The acute p
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