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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
PROVIDE a specific example for epithelial and connective tissues, how the arrangement of cells helps with tissue functioning?
How many grams of sodium dodecyl sulfate (mw = 288.37) would you dissolve in 80mLs of water to make a 20% solution of SDS?
Chancroid Chancroid, caused by Haemophilus ducreyi, is rare in the US. A single dose of azithromycin or ceftriaxone is usually effective, but may be less effective in HIV-inf
Starch Retrogradation The starch paste or solution obtained after the gelatinisation is not stable and generally produces a viscoelastic, firm and rigid gel. Structural transf
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S-Adenosyl methionine serve as a donor of methyl sets in numerous biological reactions instance for in the formation of creatine phosphate and in the synthesis of nuc
Q Are mollusc metameric beings? Exclusively analyzing this feature could it be said that molluscs are evolutionarily proximal to nematodes or to annelids? In molluscs there is
Determine some Food Sources for Copper? Foods containing more than 1 mg copper per 1000 kilocalories are considered high in copper and include green leafy vegetables, nuts, leg
Paclitaxel is a anticancer drug. It stabilizes microtubules and as a result, interferes with the normal breakdown of microtubules (you will know about microtubules in future lectur
Q. What is Dyslipidemia or Hyperlipidemia? It has been known for over five decades now that dyslipidemia is associated with increased severity and prevalence of atherosclerosis
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