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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
Explain the Combating drug-resistant diseases? The growth of drug-resistant strains of disease-causing bacteria threatens the current effectiveness of cheap antibiotics. This i
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what happens to your total peripheral resistance when you go from a warm room to cool outside air
What is the difference between self pollination and cross pollination? Which of these two modes of pollination contributes more to the plant diversity? Self pollination happens
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