Dideoxy sequencing, Biology

Assignment Help:

Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.


Related Discussions:- Dideoxy sequencing

Wbcs types, mode of action of wbcs types

mode of action of wbcs types

Formation of gemmules, Formation of Gemmules Throughout the formation...

Formation of Gemmules Throughout the formation of gemmules, masses of food - laden amoebocytes called archaeocytes feed on other cells and lay down in within them. They get s

Heart, how the blood is circulated with the help of heart

how the blood is circulated with the help of heart

Explain adverse effects of bronchospasm, Adverse Effects of Bronchospasm ...

Adverse Effects of Bronchospasm Nasal and throat discomfort can occur. Bronchospasm, sometimes severe, has been reported uncommonly in patients with reactive airway disease; za

Define reagents estimation of iron in the solution, Define Reagents Estimat...

Define Reagents Estimation of Iron in the Solution? 1. Conc. sulphuric acid (iron free) 2. Conc. potassium permanganate solution 3. Saturated potassium persulphate (K 2 S

Explain about the forces acting on a gravity retaining wall, Explain about ...

Explain about the forces acting on a gravity retaining wall. Forces Acting on a Gravity Retaining Wall: In a general way the forces that act on a gravity retaining wall. The

Ways in which genetic recombination occurs during meiosis, Define two ways ...

Define two ways in which genetic recombination occurs during meiosis. Genetic recombination happens during crossing- over and independent assortment.

Historic example of evolutionary quantitative geneticists, Define Historic ...

Define Historic Example of Evolutionary quantitative geneticists? Evolutionary quantitative geneticists have developed tools to create predictions about the short-range evoluti

Definition of breach of contract, Question 1: Mention the types of IRBs...

Question 1: Mention the types of IRBs. If the IRB disapproves clinical study, can the sponsor approach another IRB? Justify your answer. Mention different types of IRBs

How many cellular nuclei does the pollen tube have, How many cellular nucle...

How many cellular nuclei does the pollen tube of angiosperms have? What is the ploidy of each of these nuclei? The pollen tube that is the mature male gametophyte of angiosperm

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd