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Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminating dideoxynucleotides in a growing nucleic acid strand copied by DNA polymerase or the reverse transcriptase from a DNA or RNA template. Different reactions involve dideoxynucleotides containing A, C, G, or the T bases. The reaction products represent the collection of new labeled DNA strands of the varying lengths, all terminating with the dideoxynucleotide at the 3' end (at site of a complementary base in the template nucleic acid), and are separated in the polyacrylamide or urea gel to generate the sequence "ladder". This technique is more commonly used than the "Maxam-Gilbert" (chemical) sequencing.
functions of liver in frog?
Disorders of Pituitary Function: The disorders of pituitary function result in following conditions. Hypopituitarism : Growth Hormone (GH) Deficiency Hypopituitarism is
what is hybridization,and what are its different types??
Define the Coenzyme activity of nicotinic acid? Nicotinamide exists within the redox-active coenzymes, nicotinamide adenine dinucleotide (NAD) and its phosphate (NADP), which
How do contraceptive pills generally work? Contraceptive pills generally have the hormones estrogen and progesterone. If taken daily from the 4th day after menses the abnormal
Coccidioidomycosis Coccidioidomycosis is a soil-borne infection of pet, domestic, wild animals and man. The disease is caused by Coccidioides immitis, a dimorphic fungus wh
the characteristics of flying fish belong to pisces
Define the effect of Dietary fibre on Satiety? Satiety: Several investigators have speculated that ingestion of a high fibre food induces a feeling of satiety, reduces meal siz
Q. What are the allosteric enzymes? Allosteric enzymes are those that have in excess of one activation center and to which other substances, called allosteric regulators, bind.
ISOZYMES : an enzyme having different molecular forms but catalyzing the same reaction.
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