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Determine about the Manganese
Manganese performs some functions in photosynthesis and acts to regulate the intake of certain elements and their oxidation states. Several crops have been found to be lower in reducing sugar and sucrose when the Mn2+ supply is limited. Also sugar content from sugarcane is higher where there is ample manganese supply.
An excess of manganese induces chlorosis due to inactivity of iron because of its oxidation by manganese. The deficiency results in a limited development of chlorophyll and as chlorosis. Excess iron brings about symptoms of manganese deficiency. The optimum ratio of Fe/Mn is 2.0 for plant growth.
Explain the Precautions for Sub-Culturing of a Culture 1. Sterilization of inoculating wire before and after the transfer is a must. Sterilized wire should not be kept on labor
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