Determination of analyte concentration, Physics

Assignment Help:

Determination of Analyte Concentration:

One of the most common applications of spectrophotometry is to determine the concentration of an analyte in a solution. There is a relationship between concentration and absorbance.  This relationship is expressed by the Lambert-Beer law, which is more commonly known as Beer's law.  This law states that the absorbance of a light absorbing material is proportional to its concentration in solution.

A = Ebc

E = the extinction coefficient of the substance, has units of M-i  * cm-i  (unique for each substance)

b = the sample path length measured in centemeters (i.e. the width of the cuvette-almost always i cm)

c = the molar concentration of the solution (you must express concentration in terms of molarity)

It is because of this relationship that biologists measure absorption rather than transmission. The Lambert-Beer law can be used to calculate the concentration of a solution if its extinction coefficient is known.  To determine the extinction coefficient, you measure the absorbance of a known concentration of solution and then rearrange the equation to solve for E.=

Table.  Visible Spectrum and Complementary Colors

1471_Determination of Analyte Concentration.png

 


Related Discussions:- Determination of analyte concentration

Relation among electric force and electric field, In an electric field   a ...

In an electric field   a charge (Q) experiences a force F = QE. If charge is positive then force is directed in the direction of field whereas if charge is negative force acts on i

Example on electrostatic shielding, Q. During lightning, it is safer to sit...

Q. During lightning, it is safer to sit inside car than in an open ground. Why? The metal body of the car offers electrostatic shielding where the electric field is zero. Thro

Compute the threshold wavelength, Q. The work function of a metal surface i...

Q. The work function of a metal surface is 1.8 eV. Compute the threshold wavelength.  Work function W = hγ o = h C / λ 0 λ 0 = h C / W = 6.626 X 10 -34 X 3 X 10 8

The conservation of linear momentum, Hi, Good day. I enjoy watching your ph...

Hi, Good day. I enjoy watching your physics videos, but I have a question and was wondering if you can answer it for me showing the working. the question is as follows: A flat truc

Determine the terminal velocity, A 10 kg block slides down a smooth incline...

A 10 kg block slides down a smooth inclined surface as shown in Figure 1. Determine the terminal velocity of the block if the 0.1 mm gap between the block and the surface contains

Standing waves on a string, When a string under tension is set into vibrati...

When a string under tension is set into vibration, transverse harmonic rays propagate along its length. When the size of string is same, reflected rays will also exist. The operati

What is instantaneous speed and velocity, Instantaneous velocity and speed ...

Instantaneous velocity and speed are explained at a certain instant and are shown by

Describe snells law, Out of ordinary and extra-ordinary waves, which wave o...

Out of ordinary and extra-ordinary waves, which wave obeys Snell's law?

Thermodynamics, The internal energy change when a system goes from state A ...

The internal energy change when a system goes from state A to B is 40 kJ/mole. If the system goes from A to B by a reversible path and returns to state A by an irreversible path wh

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd