Determination of analyte concentration, Physics

Assignment Help:

Determination of Analyte Concentration:

One of the most common applications of spectrophotometry is to determine the concentration of an analyte in a solution. There is a relationship between concentration and absorbance.  This relationship is expressed by the Lambert-Beer law, which is more commonly known as Beer's law.  This law states that the absorbance of a light absorbing material is proportional to its concentration in solution.

A = Ebc

E = the extinction coefficient of the substance, has units of M-i  * cm-i  (unique for each substance)

b = the sample path length measured in centemeters (i.e. the width of the cuvette-almost always i cm)

c = the molar concentration of the solution (you must express concentration in terms of molarity)

It is because of this relationship that biologists measure absorption rather than transmission. The Lambert-Beer law can be used to calculate the concentration of a solution if its extinction coefficient is known.  To determine the extinction coefficient, you measure the absorbance of a known concentration of solution and then rearrange the equation to solve for E.=

Table.  Visible Spectrum and Complementary Colors

1471_Determination of Analyte Concentration.png

 


Related Discussions:- Determination of analyte concentration

Find the statement true or false and why, A bathtub is filled to the brim w...

A bathtub is filled to the brim with ice cold water and has an iceberg floating in it. It's a big bathtub the iceberg, of course, project above the surface of the tube when the ice

How to improve power factor, Step to improve Power Factor By using stat...

Step to improve Power Factor By using static capacitor: Static capacitor is usually used to better the power factor of the power system. As the capacitor draws leading curre

Define normal force (fnorm), What is Normal Force?? Force exerted upon...

What is Normal Force?? Force exerted upon an object that is in get in touch with another stable object. If a book is resting upon a surface, then the surface is exerting an up

Compute the feedback ratio, Q. When the negative feedback is practical to a...

Q. When the negative feedback is practical to an amplifier of gain 50 the gain falls to 25.  Compute the feedback ratio? Af = A / 1 + Aβ 25 = 50 / 1 + 50 β 1 = 2 / 1 + 50

Acceleration, suppose you are running and want to stop at once. surely you ...

suppose you are running and want to stop at once. surely you will have to produce negative acceleration in your speed. can you tell from where does the necessary force come ?

Refraction law, Refraction law: For a wavefront travelling by a bounda...

Refraction law: For a wavefront travelling by a boundary among two media, the first along a refractive index of n 1 , and the other with one of n­, the angle of incidence thet

Determine the load power factor, Two wattmeters indicate 10 kW and 3 kW res...

Two wattmeters indicate 10 kW and 3 kW respectively when linked to measure the input power to a 3-phase balanced load, the reverse switch being operated on the meter showing the 3k

What is the activity of the radioactive source, A 2.0 kg tumor is being irr...

A 2.0 kg tumor is being irradiated by a radioactive source. The tumor receives an absorbed dose of 12 Gy in a time of880 s. Each disintegration of the radioactive source produces a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd