Determination of analyte concentration, Physics

Assignment Help:

Determination of Analyte Concentration:

One of the most common applications of spectrophotometry is to determine the concentration of an analyte in a solution. There is a relationship between concentration and absorbance.  This relationship is expressed by the Lambert-Beer law, which is more commonly known as Beer's law.  This law states that the absorbance of a light absorbing material is proportional to its concentration in solution.

A = Ebc

E = the extinction coefficient of the substance, has units of M-i  * cm-i  (unique for each substance)

b = the sample path length measured in centemeters (i.e. the width of the cuvette-almost always i cm)

c = the molar concentration of the solution (you must express concentration in terms of molarity)

It is because of this relationship that biologists measure absorption rather than transmission. The Lambert-Beer law can be used to calculate the concentration of a solution if its extinction coefficient is known.  To determine the extinction coefficient, you measure the absorbance of a known concentration of solution and then rearrange the equation to solve for E.=

Table.  Visible Spectrum and Complementary Colors

1471_Determination of Analyte Concentration.png

 


Related Discussions:- Determination of analyte concentration

What is Neural coding? Explain Neural coding, Neural coding is a neuroscien...

Neural coding is a neuroscience based field mainly concerned with characterizing the relationship b/w the stimulus & the individual and ensemble neuronal responses or the relations

How will this image be dissimilar from the one obtained, The image of a can...

The image of a candle is produced by a convex lens on a screen. The lower half of the lens is painted black to make it completely opaque. Illustrate the ray diagram to show the ima

Determine the change in the momentum of the ball, A girl throws a 0.1 kilog...

A girl throws a 0.1 kilogram ball at a wall.  The ball hits the wall perpendicularly with a velocity of 5 meters per second and then bounces straight back with a velocity of 4

Electrostatic force and gravitational force, compare the electrostatic forc...

compare the electrostatic force between two protons to the gravitational force acting on them?

Determine the Effect of air resistance, An object in free fall has an accel...

An object in free fall has an acceleration of 9.80 m/s 2 assuming that there is no air resistance. What is the speed of an object dropped from the top of a tall cliff 3.50 s a

How many significant digits in 100m, How many significant digits are there ...

How many significant digits are there in each of the following measurements? a. 100 m 1 b. 0.0023 m/s 2 c. 100.1 m 4 d. 2.0023 5

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd