Determination of analyte concentration, Physics

Assignment Help:

Determination of Analyte Concentration:

One of the most common applications of spectrophotometry is to determine the concentration of an analyte in a solution. There is a relationship between concentration and absorbance.  This relationship is expressed by the Lambert-Beer law, which is more commonly known as Beer's law.  This law states that the absorbance of a light absorbing material is proportional to its concentration in solution.

A = Ebc

E = the extinction coefficient of the substance, has units of M-i  * cm-i  (unique for each substance)

b = the sample path length measured in centemeters (i.e. the width of the cuvette-almost always i cm)

c = the molar concentration of the solution (you must express concentration in terms of molarity)

It is because of this relationship that biologists measure absorption rather than transmission. The Lambert-Beer law can be used to calculate the concentration of a solution if its extinction coefficient is known.  To determine the extinction coefficient, you measure the absorbance of a known concentration of solution and then rearrange the equation to solve for E.=

Table.  Visible Spectrum and Complementary Colors

1471_Determination of Analyte Concentration.png

 


Related Discussions:- Determination of analyte concentration

Determine average kinetic energy of each type of molecule, Determine the av...

Determine the average kinetic energy of each type of molecule? A cylinder contains a mixture of helium and argon gas in equilibrium at temperature of 149°C. (a) What is the a

Schrodinger''s time independent wave equation: free particle , Q.   Write d...

Q.   Write down the Schrodinger's time independent wave equation for a free particle confined in one dimensional box of size a. Obtain eigen values and normalized wave functions fo

Reverse characteristic of a zener diode, Reverse Characteristic of a Zener ...

Reverse Characteristic of a Zener diode Reset voltage to  zero.  Connect  zener diode as shown in fig. and reverse characteristics are demonstrated in fig. Raise voltag

Reducing friction by an air stream, Reducing friction by an air stream ...

Reducing friction by an air stream Cut out a disc of cardboard about 10 cm in diameter. With a red-hot pin, burn a hole by the centre. Saw a small cotton reel in half and glue

What does hull speed mean, Q. Illustrate what does hull speed mean? An...

Q. Illustrate what does hull speed mean? Answer:- Hull speed- It is the theoretical limit of a sail craft's speed. It is able to be approximated by the following formula

Setellite, Discusse about motion of satellite

Discusse about motion of satellite

Explain fusion reaction, Fusion Reaction: In nuclear fusion light nucle...

Fusion Reaction: In nuclear fusion light nuclei are brought together to form a relation heavier nucleus. They energy free in nuclear fusion is larger than that free in fission.

Illustrate properties of electromagnetic waves, Q. Illustrate what two prop...

Q. Illustrate what two properties of electromagnetic waves are used to attach info to radio signals? Answer:- By changing the amplitude of a radio wave you can encode info

What is the acceleration due to gravity on mars, Ngan has a weight of 314.5...

Ngan has a weight of 314.5 N on Mars and a weight of 833.0 N on Earth. a. What is Ngan's mass on Mars? b. What is the acceleration due to gravity on Mars, g Mars ?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd