Determination of analyte concentration, Physics

Assignment Help:

Determination of Analyte Concentration:

One of the most common applications of spectrophotometry is to determine the concentration of an analyte in a solution. There is a relationship between concentration and absorbance.  This relationship is expressed by the Lambert-Beer law, which is more commonly known as Beer's law.  This law states that the absorbance of a light absorbing material is proportional to its concentration in solution.

A = Ebc

E = the extinction coefficient of the substance, has units of M-i  * cm-i  (unique for each substance)

b = the sample path length measured in centemeters (i.e. the width of the cuvette-almost always i cm)

c = the molar concentration of the solution (you must express concentration in terms of molarity)

It is because of this relationship that biologists measure absorption rather than transmission. The Lambert-Beer law can be used to calculate the concentration of a solution if its extinction coefficient is known.  To determine the extinction coefficient, you measure the absorbance of a known concentration of solution and then rearrange the equation to solve for E.=

Table.  Visible Spectrum and Complementary Colors

1471_Determination of Analyte Concentration.png

 


Related Discussions:- Determination of analyte concentration

Describe q-factor in oscillation, Describe Q-factor in oscillations? How do...

Describe Q-factor in oscillations? How does it relay on damping

Electronics, if you want to amplify input signal of +-20mV amplitude and a ...

if you want to amplify input signal of +-20mV amplitude and a 100 MHz frequency,which parameters of OP-amp play a significant role? what should be the values of the parameters?just

Places where friction occurs, Places where friction occurs Locate the p...

Places where friction occurs Locate the places on several mechanical devices where parts rub together. Roller skates, pulleys and the wheels of toys often required oil. Try to

Screw gauge, i want to do experiment online

i want to do experiment online

Calculate the heat removal factor, Calculate the heat-removal factor and th...

Calculate the heat-removal factor and the duct flow factor for a collector having an overall heat loss coefficient of 6 W/ (m 2 .K) constructed of aluminum fins and tubes. Tube to

Nicol prism, Working of nicol prism and its uses as polarizer and analyzer

Working of nicol prism and its uses as polarizer and analyzer

Kinematics, A body moving in the positive x direction passes the origin at ...

A body moving in the positive x direction passes the origin at time t=0. Between t=0 and t=1 second, the body has a constant speed of 24 meters per second. At t=1 second, the body

A foucault pendulum to show the rotation of the earth, A  Foucault pendulum...

A  Foucault pendulum to show the rotation of the earth A G-clamp with a ball bearing soldered to the inside of the jaw makes a excellent support for a Foucault Pendulum. It is

Determine the change in entropy of the system, Two compartments each of 1 m...

Two compartments each of 1 m 3 capacity are connected by a valve and insulated from the surroundings and from each other. One compartment contains saturated steam at 683.6 kPa and

Determine a single-phase transformer, A single-phase transformer has 2400 t...

A single-phase transformer has 2400 turns on the primary and 600 turns on the secondary. Its no-load current is 4A at a power factor of 0.25 lagging. Suppose the volt drop

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd