Determination of analyte concentration, Physics

Assignment Help:

Determination of Analyte Concentration:

One of the most common applications of spectrophotometry is to determine the concentration of an analyte in a solution. There is a relationship between concentration and absorbance.  This relationship is expressed by the Lambert-Beer law, which is more commonly known as Beer's law.  This law states that the absorbance of a light absorbing material is proportional to its concentration in solution.

A = Ebc

E = the extinction coefficient of the substance, has units of M-i  * cm-i  (unique for each substance)

b = the sample path length measured in centemeters (i.e. the width of the cuvette-almost always i cm)

c = the molar concentration of the solution (you must express concentration in terms of molarity)

It is because of this relationship that biologists measure absorption rather than transmission. The Lambert-Beer law can be used to calculate the concentration of a solution if its extinction coefficient is known.  To determine the extinction coefficient, you measure the absorbance of a known concentration of solution and then rearrange the equation to solve for E.=

Table.  Visible Spectrum and Complementary Colors

1471_Determination of Analyte Concentration.png

 


Related Discussions:- Determination of analyte concentration

Tipler machine, Tipler machine: A solution to Einstein's equations of ...

Tipler machine: A solution to Einstein's equations of general relativity that let time travel. A very dense (on the order of the density of neutron star matter), infinitely-lo

Determine a single-phase transformer, A single-phase transformer has 2400 t...

A single-phase transformer has 2400 turns on the primary and 600 turns on the secondary. Its no-load current is 4A at a power factor of 0.25 lagging. Suppose the volt drop

Optics, Michlson interferometer

Michlson interferometer

What is the symbol of the charge on the ball, Consider two identical plasti...

Consider two identical plastic foam balls each one having a thin sheath of aluminium foil. One of the balls has a few positive charges and the other has the same amount of negative

Magnitude of k.e. in an orbit, Magnitude of K.E. in an orbit is equal to: ...

Magnitude of K.E. in an orbit is equal to: (1)Half of the potential energy     (2)Twice of the potential energy       (3)One fourth of the potential energy   (4 ) None

Excitation energy and potential, When the electron is known energy from ext...

When the electron is known energy from external source, it goes to higher energy level. This process is called as excitation. The minimum energy needed to excite an atom is know

Find out the shear force in kn rounded to 1 dp, Determine the Shear Force i...

Determine the Shear Force in kN rounded to 1 DP and Bending Moment in kN.m rounded to 1 DP at point C in the beam when the UDL for AC on the beam is P52 kN/m and the maximum value

Electricity & Magnetism, A charge q1 is 9.00um is at the origin, and a char...

A charge q1 is 9.00um is at the origin, and a charge q2 is -4.00um is on the x- axis 0.30m from the origin. find the electric field strength at point P, which is on the y - axis 0.

The power required to operate the pump, An ideal Rankine cycle uses water a...

An ideal Rankine cycle uses water as a working fluid, which circulates at a rate of 80 kg/s. The boiler pressure is 6 Mpa, and the condenser pressure is 10 kpa. The water enters th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd