Determination of analyte concentration, Physics

Assignment Help:

Determination of Analyte Concentration:

One of the most common applications of spectrophotometry is to determine the concentration of an analyte in a solution. There is a relationship between concentration and absorbance.  This relationship is expressed by the Lambert-Beer law, which is more commonly known as Beer's law.  This law states that the absorbance of a light absorbing material is proportional to its concentration in solution.

A = Ebc

E = the extinction coefficient of the substance, has units of M-i  * cm-i  (unique for each substance)

b = the sample path length measured in centemeters (i.e. the width of the cuvette-almost always i cm)

c = the molar concentration of the solution (you must express concentration in terms of molarity)

It is because of this relationship that biologists measure absorption rather than transmission. The Lambert-Beer law can be used to calculate the concentration of a solution if its extinction coefficient is known.  To determine the extinction coefficient, you measure the absorbance of a known concentration of solution and then rearrange the equation to solve for E.=

Table.  Visible Spectrum and Complementary Colors

1471_Determination of Analyte Concentration.png

 


Related Discussions:- Determination of analyte concentration

What is electrostatic shielding, Q. What is electrostatic shielding? i...

Q. What is electrostatic shielding? i) It is the method of isolating a certain region of space from external field. ii) It is basis on the fact that electric field inside a

Illustrate double-beam oscilloscope, For the double-beam oscilloscope displ...

For the double-beam oscilloscope displays shown in Figure, determine (a) their frequency (b) their r.m.s. values (c) their phase difference. The 'time/cm' switch is on 10

Standing waves on a string, When a string under tension is set into vibrati...

When a string under tension is set into vibration, transverse harmonic rays propagate along its length. When the size of string is same, reflected rays will also exist. The operati

Explain direct current (dc), Direct Current (DC): Such a current can be...

Direct Current (DC): Such a current can be getting by connecting the two ends of a conductor to the terminals of the battery. This current doesn't change the direction

Ouestion, what is different between acid and base?

what is different between acid and base?

Laplace equation, Laplace equation (P. Laplace): For steady-state heat...

Laplace equation (P. Laplace): For steady-state heat conduction in one dimension, the temperature distribution is the resolution to Laplace's equation, that states the second

Physical significance of gradient of a scalar function, Explain the physica...

Explain the physical significance of gradient of a scalar function?

Superposition principle of forces and waves, Superposition principle of for...

Superposition principle of forces The net force onto a body is equivalent to the total forces impressed upon it. superposition principle of states The resultant qu

Explain musical sounds, Musical Sounds: Sound that produces pleasant ef...

Musical Sounds: Sound that produces pleasant effects on our ear is known as musical sound. Such as sound of flute, violin.

Digital image, What is the raw file size for an image of 80 rows and 100 co...

What is the raw file size for an image of 80 rows and 100 columns, with pixels on 14 bits and with an extra row for the header? The minimum and maximum values observed in a  low

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd