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Define Ziehl-Neelsen Staining Technique?
Ziehl-Neelsen staining is used for staining these bacteria. The technique was discovered first by Robert Koch during his pioneering investigations on tuberculosis and was the fore-runner of Ziehl-Neelsen stain used today for diagnosis of these organisms in laboratory. Acid fast bacteria are called so because once these cells get stained with basic fuchsin, these resist decolourization with acid alcohol as can be seen in margin illustiation. This acid fastness red stain is due to high lipid content particularly of mycolic acid in their cell wall. Mycolic acid, is a group of branched chain hydroxyl lipids, which bind covalently to peptidoglycan of cell wall giving the cell surface a waxy, hydrophobic consistency.
(1) The 7,893 bp plasmid shown below was digested with a series of restriction enzymes (P = PstI, B = BamHI, S = SalI, X = XbaI). Answer the questions below. a) Indicate
How does photoperiodism affect the flowering of some plants? The Flowering is an easy and typical to observe example of photoperiodism. Most of flowering plants flower only dur
Q. Of which type of defense cell do viral infections stimulate the multiplication? The main leukocytes that generally participate and multiply in the defense against viral infe
Why Deficiency of Folate take place? If there is inadequate dietary folate, the activity of both the DNA and the methylation cycles, described above, will be reduced. A decrea
Homozygous and Weterozygous In an individual two identical alleles may exist [or a given character and, hence, the individual is referred to as Homozygous (e.g. AA and aa). If
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI
What are Flavanols? Examples are catechin and epicatechin. You may have heard of the benefits of tea. Some of them are due to gallic acid, which is combined with epicatechin. F
Explain fontan operation and ross operation in heart dieases? Fontan Operation: The Fontan principle involves redirecting the whole systemic return into the lungs bypassing t
Soil Water Soil water relationship is very important. In the fist place the supply of large quantities of water is necessary to satisfy the evapo-transpiration requirements of
Differences between cells of Prokaryotes and Eukaryotes The prokaryotes also differ from the eukaryotes in many other ways, as you can see from Table. Table: Differences
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