Define sample titration for estimation of reducing sugar, Biology

Assignment Help:

Define Sample Titration for Fehling Soxhlet Method

Dilute the given sample glucose solution in 100 ml volumetric flask to the mark with distilled water. Pipette 5 ml of Fehling's A and and 5 ml of Fehling B solution into a 100 ml conical flask. Add 4-5 ml of the diluted sugar solution from the burette and heat over a wire gauze until the solution starts boiling. Boil for about 15-25 seconds and add further amounts of sugar solution in small volumes until only a faint blue colour is seen. Add 1 ml of 0.2% methylene blue solution. Continue heating and complete the titration by adding sugar solution drop by drop ensuring that there is a few seconds of gap between each addition so that the titration doesn't go beyond three minutes.

The contents of the conical flask should be continuously boiling during the titration. This is a pilot reading and it will be slightly higher than the actual titer value. For the subsequent readings, add sugar solution 1 ml less than the amount required to reduce 10 ml of Fehlings's solution (this will be 1 ml less than pilot reading). Heat the mixture to boiling point over a wire gauze and continue boiling for two minutes. Without removing the burner add 1 ml of methylene blue solution and complete the titration within another minute by adding small amounts of sugar solution (2-3 drops) at a time till the end point is reached. Repeat the titrations till concordant titer values are obtained. Calculate the amount of glucose present in the given sample solution.


Related Discussions:- Define sample titration for estimation of reducing sugar

Why do roots of many swamp plants have a special morphology, Why do roots o...

Why do roots of many swamp plants have a special morphology? Swamp and marsh plants usually present supporting roots that ramify from portions of the stem above the ground help

What is a biome, What is a biome? A biome is the prevailing ecosystem c...

What is a biome? A biome is the prevailing ecosystem constituted by similar abiotic and biotic factors present in one or more regions of the planet.

Explain adverse effects of cidofovir, Explain Adverse Effects of Cidofovir ...

Explain Adverse Effects of Cidofovir About 25% of patients discontinue cidofovir because of adverse effects such as nephrotoxicity, neutropenia and metabolic acidosis. Iritis,

Class Pisces, need different types of information about fishes and their pi...

need different types of information about fishes and their pictures

What is the need of monitoring and surveillance, What is the need of Monito...

What is the need of Monitoring and Surveillance? Monitoring, we know, is the act of observing something and sometimes keeping a .record of it while surveillance is a repeated s

Explain major role in contributing towards motivation, Major role in contri...

Major role in contributing towards motivation. Psychological factors: Depression, anxiety or phobia induced by illness,  life- style changes or medication effects, may hinder

Where can rna be found within cells, Where can RNA be found within cells? ...

Where can RNA be found within cells? In the eukaryote cell nucleus RNA can be found dispersed in the nuclear fluid, with DNA, and as the main constituent of the nucleolus. In c

How you show that yeast acts on sugar, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Are protozoans presenting pulsatile, Q. Are protozoans presenting pulsatile...

Q. Are protozoans presenting pulsatile or contractile vacuoles easily found in fresh or in salt water? Fresh water is the less concentrated of solutes than sea water and it fre

What are the main techniques of genetic engineering, At the present level o...

At the present level of the biotechnology what are the main techniques of genetic engineering? The major techniques of genetic engineering today are: the recombinant DNA techno

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd