Define sample titration for estimation of reducing sugar, Biology

Assignment Help:

Define Sample Titration for Fehling Soxhlet Method

Dilute the given sample glucose solution in 100 ml volumetric flask to the mark with distilled water. Pipette 5 ml of Fehling's A and and 5 ml of Fehling B solution into a 100 ml conical flask. Add 4-5 ml of the diluted sugar solution from the burette and heat over a wire gauze until the solution starts boiling. Boil for about 15-25 seconds and add further amounts of sugar solution in small volumes until only a faint blue colour is seen. Add 1 ml of 0.2% methylene blue solution. Continue heating and complete the titration by adding sugar solution drop by drop ensuring that there is a few seconds of gap between each addition so that the titration doesn't go beyond three minutes.

The contents of the conical flask should be continuously boiling during the titration. This is a pilot reading and it will be slightly higher than the actual titer value. For the subsequent readings, add sugar solution 1 ml less than the amount required to reduce 10 ml of Fehlings's solution (this will be 1 ml less than pilot reading). Heat the mixture to boiling point over a wire gauze and continue boiling for two minutes. Without removing the burner add 1 ml of methylene blue solution and complete the titration within another minute by adding small amounts of sugar solution (2-3 drops) at a time till the end point is reached. Repeat the titrations till concordant titer values are obtained. Calculate the amount of glucose present in the given sample solution.


Related Discussions:- Define sample titration for estimation of reducing sugar

What is the approximate number, What would be the approximate number of ami...

What would be the approximate number of amino acids of a protein with a mass of 203 kd? Assume an average molecular weight for amino acids of 110 grams/mol.

Microorganisms on basis of oxygen requirement for growth, Q. Microorganisms...

Q. Microorganisms on basis of oxygen requirement for growth? On basis of oxygen requirement for growth: - Obligate Aerobes: Require oxygen for growth and multiplication e.g

What is the structural formula of glycerol, What is the structural formula ...

What is the structural formula of glycerol? To which organic function do these molecules belong? Glycerol is a linear chain of 3 carbons; the central carbon is bound to one hyd

How many chromosomes and dna molecules found per cell, A cell in G1 of inte...

A cell in G1 of interphase has 12 chromosomes. How many chromosomes and DNA molecules will be found per cell when this original cell progresses to the G2?

Cyclins and cyclin-dependent kinases, Rhythmic fluctuations in the abundanc...

Rhythmic fluctuations in the abundance and activity of cell-cycle control molecules pace the events of the cell cycle. • Kinase - a protein which activates or deactivates another

How is the cerebrum anatomically divided, Q. How is the cerebrum anatomical...

Q. How is the cerebrum anatomically divided? The cerebrum is divided into two cerebral hemispheres, the left and the right. Each hemisphere is made of four cerebral lobes: pari

Immunofluorescence test and its applications, Question 1 Discuss the Follo...

Question 1 Discuss the Following                    a) Immune response                    b) Immunofluorescence test and its applications Question 2 Answer the following ques

Explain shear pin model, Q. Explain Shear Pin Model? Shear Pin Model: I...

Q. Explain Shear Pin Model? Shear Pin Model: In this type, a shearing force is applied using a pin to shear the food Product. We are rupturing the element of the food product b

Symptoms of dyspepsia, Q. Symptoms of dyspepsia? The life style modific...

Q. Symptoms of dyspepsia? The life style modification in terms of work schedules, eating behaviour like consuming meals when extremely tired, quitting alcohol and smoking would

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd