Define sample titration for estimation of reducing sugar, Biology

Assignment Help:

Define Sample Titration for Fehling Soxhlet Method

Dilute the given sample glucose solution in 100 ml volumetric flask to the mark with distilled water. Pipette 5 ml of Fehling's A and and 5 ml of Fehling B solution into a 100 ml conical flask. Add 4-5 ml of the diluted sugar solution from the burette and heat over a wire gauze until the solution starts boiling. Boil for about 15-25 seconds and add further amounts of sugar solution in small volumes until only a faint blue colour is seen. Add 1 ml of 0.2% methylene blue solution. Continue heating and complete the titration by adding sugar solution drop by drop ensuring that there is a few seconds of gap between each addition so that the titration doesn't go beyond three minutes.

The contents of the conical flask should be continuously boiling during the titration. This is a pilot reading and it will be slightly higher than the actual titer value. For the subsequent readings, add sugar solution 1 ml less than the amount required to reduce 10 ml of Fehlings's solution (this will be 1 ml less than pilot reading). Heat the mixture to boiling point over a wire gauze and continue boiling for two minutes. Without removing the burner add 1 ml of methylene blue solution and complete the titration within another minute by adding small amounts of sugar solution (2-3 drops) at a time till the end point is reached. Repeat the titrations till concordant titer values are obtained. Calculate the amount of glucose present in the given sample solution.


Related Discussions:- Define sample titration for estimation of reducing sugar

State the types of bone density, State the types of bone density Misch ...

State the types of bone density Misch classified bone density into following types (1988) D1. Dense cortical bone- which is almost never observed in maxilla and approximatel

What is endocrine system explain, What is Endocrine System explain ? Th...

What is Endocrine System explain ? The endocrine system consists of glands, organs that secrete hormones. Endocrine glands are tissues that produce secretions within the body,

Factors causing short-term changes in intra-ocular pressure, What Factors c...

What Factors causing short-term changes in intra-ocular pressure 1) Arterial blood pressure 2) Systemic venous pressure (SVP) 3) Mechanical pressure 4) Plasma osmolari

Respiration in annelids, Describe the different types of respiratory organs...

Describe the different types of respiratory organs found in annelids.

Explain the process of transposition of the great arteries, Transposition o...

Transposition of the Great Arteries with VSD and Left Ventricular Outflow Tract Obstruction: The presence of such a condition has to be surgically corrected title is differenc

Neuston - aquatic ecosystem, Neuston - Aquatic Ecosystem These are una...

Neuston - Aquatic Ecosystem These are unattached organisms which live at the air-water interface such as floating plants and several types of animals. Some spend most of their

Skeletal system - cerebro-spinal fluid, Cerebro-spinal Fluid - Formed m...

Cerebro-spinal Fluid - Formed mainly by choroid plexus and ependyma of ventricles. It comes out of medulla oblongeta by foramen of magandie and lushka . Slightly alkalin

Explain procedure for performing the completed coliform test, Explain Proce...

Explain Procedure for Performing the Completed Coliform Test? Completed test is the last step in the Coliform test procedure which is described herewith. Conduct the exercise f

Biological valves, Biological Valves :  Biological valves are available fo...

Biological Valves :  Biological valves are available for all positions. For mitral position they are usually mounted on a stent (stent mounted) whereas for aortic position they ma

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd