Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Sample Titration for Fehling Soxhlet Method
Dilute the given sample glucose solution in 100 ml volumetric flask to the mark with distilled water. Pipette 5 ml of Fehling's A and and 5 ml of Fehling B solution into a 100 ml conical flask. Add 4-5 ml of the diluted sugar solution from the burette and heat over a wire gauze until the solution starts boiling. Boil for about 15-25 seconds and add further amounts of sugar solution in small volumes until only a faint blue colour is seen. Add 1 ml of 0.2% methylene blue solution. Continue heating and complete the titration by adding sugar solution drop by drop ensuring that there is a few seconds of gap between each addition so that the titration doesn't go beyond three minutes.
The contents of the conical flask should be continuously boiling during the titration. This is a pilot reading and it will be slightly higher than the actual titer value. For the subsequent readings, add sugar solution 1 ml less than the amount required to reduce 10 ml of Fehlings's solution (this will be 1 ml less than pilot reading). Heat the mixture to boiling point over a wire gauze and continue boiling for two minutes. Without removing the burner add 1 ml of methylene blue solution and complete the titration within another minute by adding small amounts of sugar solution (2-3 drops) at a time till the end point is reached. Repeat the titrations till concordant titer values are obtained. Calculate the amount of glucose present in the given sample solution.
Does pH affect the enzyme activity? The concentration of hydrogen ions in solution affects the enzyme activity. Every enzyme has maximal efficiency under an optimum pH. As p
Method to Estimate Phytochrome Since there is no chemical test, the only method available to test and estimate phytochrome is the spectrophotometric method. Firstly, the diffe
Define Heart Rate (HR) Method - aerobic capacity? An alternative method for determining aerobic capacity involves the measurement of heart rate. Heart rate is linearly associa
Q. What are the three phases into which the HIV infection is often divided? The HIV infection is frequently divided into three phases the acute phase, from the infection to 1 u
Implementation The planned goals and strategies are translated into nursing intervention by means of the nursing care plan. It is essential that the client and family par
Q. Explain the Rapport Building? Maintaining good relationship with the client is very important in counselling. This will help you to gain trust, confidence of the client and
what are the types of pseudopodia
Q. Explain Restricted fragment polymorphism? This method makes use of a group of enzymes termed restriction enzymes which recognise particular sequence of DNA and act as "molec
What is Physiologically corrected transposition of arteries? Physiologically corrected Transposition of the great arteries (ZTGA): Atrio- ventricular connections are also inapp
Compare and contrast covalent and ionic bonds in molecules, give an example of each.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd