Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Sample Titration for Fehling Soxhlet Method
Dilute the given sample glucose solution in 100 ml volumetric flask to the mark with distilled water. Pipette 5 ml of Fehling's A and and 5 ml of Fehling B solution into a 100 ml conical flask. Add 4-5 ml of the diluted sugar solution from the burette and heat over a wire gauze until the solution starts boiling. Boil for about 15-25 seconds and add further amounts of sugar solution in small volumes until only a faint blue colour is seen. Add 1 ml of 0.2% methylene blue solution. Continue heating and complete the titration by adding sugar solution drop by drop ensuring that there is a few seconds of gap between each addition so that the titration doesn't go beyond three minutes.
The contents of the conical flask should be continuously boiling during the titration. This is a pilot reading and it will be slightly higher than the actual titer value. For the subsequent readings, add sugar solution 1 ml less than the amount required to reduce 10 ml of Fehlings's solution (this will be 1 ml less than pilot reading). Heat the mixture to boiling point over a wire gauze and continue boiling for two minutes. Without removing the burner add 1 ml of methylene blue solution and complete the titration within another minute by adding small amounts of sugar solution (2-3 drops) at a time till the end point is reached. Repeat the titrations till concordant titer values are obtained. Calculate the amount of glucose present in the given sample solution.
1. Name four experimental techniques to determine protein-protein interactions other than X-ray & NMR techniques. 2. How common are Coiled-coil motifs? Where are they found? Giv
Complications : IABP can compromise blood flow to the leg at the time of insertion, pumping or after removal of balloon. It may also cause perforation, bleeding, thrombosis, embol
Define the Effect of Deficiency of Vitamin K? Both excess and low intakes can have serious implications on human health, although such conditions arise rarely, these can often
Define How to Prevent the Cancer? A continuing and urgent need is there to take all steps to prevent cancer. Cancer rates are set to increase another 50 percent by the year 202
Surgery and Prosthetic Phase Implants are uncovered Impression recorded Abutment placed Provisional prosthesis followed by Definitive prosthesis
Q. What are the analyses provided by the study of human age pyramids? The study of human age pyramids able to provide the following analyses, proportion of individuals at an ec
Explain some important phospholipids The chemical structure of phosphatidylcholine (lecithin) illustrated here in Figure is typical of the phosphatides found in the brai
Define minerals Definition of minerals, as it applies to food and nutrition usually refers to the elements other than carbon, hydrogen, oxygen and nitrogen that are present in
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
STRUCTUR E OF SPERMATOZOAN Structure of sperm is divided into 3 parts - (1) Head (2) Middle piece (3) Tail 1 . HEAD
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd