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Define reagents required and methodology for Benedict Test?
Reagents Required
- Sugar solutions of glucose, fructose, galactose, maltose, lactiose, sucrose and starch.
- Benedict's reagent (containing copper sulphate, sodium citrate and sodium carbonate).
Methodology
To 5 ml of Benedict's reagent in a test tube add 8-10 drops of glucose solution. Observe the test tube carefully. Repeat the test with fructose and galactose.
At 1 AM, an impermeable membrane separates a 1 liter solution of 2M KCl in the left compartment from a 1 liter solution containing both 1M NaCl and 1M KCl in the right compartment.
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