Define procedure for determination of fungal and yeast count, Biology

Assignment Help:

Define Procedure for Determination of Fungal and Yeast Count?

Now carry out the exercise following the steps enumerated herewith:

1. Shake the food sample and aseptically transfer 1 ml of it to a 9 ml dilution blank tube marked 10-1 . Similarly prepare further tenfold dilutions till 10-6.

2. Dispense 0.1 ml of each dilution on to the labeled agar plates.

3. Spread the inoculum evenly on the plates using a sterile bent glass spreader. Sterilization of a spreader is done by dipping it in the alcohol jar and passing through the flame. Caution - Always keep the alcohol away from the flame.

4. Incubate plates at 28+2°C for 4-5 days undisturbed.

5. Count the number of yeasts and moulds. Yeast colonies are similar to bacteria whereas mould colonies are larger and fuzzier. Counting can be done from underside of plates if moulds are overgrown.

6. Calculate the CFU/ml of the food sample. Try to identify the fungi.


Related Discussions:- Define procedure for determination of fungal and yeast count

Explain the pathogens of forest insects, Explain the Pathogens of forest in...

Explain the Pathogens of forest insects? Caterpillars that defoliate forest trees have main economic impacts. Many such species go through periodic outbreaks. The forces which

Moss stage - xerarch, Moss Stage - Xerarch The accumulation of soil, p...

Moss Stage - Xerarch The accumulation of soil, particularly in the crevices and depressions of rock favours the growth of certain xerophytic mosses, e.g., species of Polytrich

Conditions requiring rapid treatment of hypertension, List of Conditions Re...

List of Conditions Requiring Rapid Treatment of Hypertension 1) Cardiac: • Acute aortic dissection • Acute left ventricular failure • Acute or evolving myocardial i

Define skirt fold thickness (spt) method, Define Skirt Fold Thickness (S...

Define Skirt Fold Thickness (SPT) Method? Skin fold measurement is the most widely used field method of body composition assessment. The skin fold (SKF) is an indirect measu

Tanco beans plant, what are the origin, morphology, active constituents, us...

what are the origin, morphology, active constituents, uses and market preparation of tanco beans

What is an effect of increased temperature, What is the effect of increased...

What is the effect of increased temperature on a non enzyme catalyzed reaction? What are the effects of increased temperature on an enzyme catalyzed reaction?

Demonstration of diversity of plant and animal - taxonomy, Demonstration of...

Demonstration of Diversity of Plant and Animal - Taxonomy A third objective is the demonstration of tremendous diversity of the plant and animal world and its relation to man's

Does the dna replication occur in the cell division, Q. Does the DNA replic...

Q. Does the DNA replication occur in the cell division? Yes. The DNA replication occurs in mitosis as well in the meiosis.

What is the difference between spermatids and sperm cells, What is the diff...

What is the difference between spermatids and sperm cells? What is the name of the transformation of spermatids into sperm cells? Sperm cells (the male gametes) are matured spe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd