Define plate counts and colony forming unit, Biology

Assignment Help:

Define Plate Counts and Colony Forming Unit?

Plate counts is the most frequently used method to measure microbial numbers. The technique, however, can be used only for those microorganisms, which are able to grow and reproduce on microbial media. Here a diluted sample of microbes is dispersed over suitable nutrient agar medium. There are two ways of performing a plate count - the spread plate and pour plate technique about which we have already studied in the last practical. Look up these techniques once again. Each microorganism then produces a distinct colony. Original number of viable microorganism in a sample is then calculated from the number of colonies formed and the sample dilution used. The results are reported in form of colony forming units (CFU) per ml of the sample.

Colony forming unit/ml can be expressed as:

CFU/ml = (No. of colonies formed * dilution factor) / Volume Sampled


Related Discussions:- Define plate counts and colony forming unit

What is cardiac tamponade, Q. What is Cardiac tamponade? Cardiac tampon...

Q. What is Cardiac tamponade? Cardiac tamponade is that situation where increase in pericardial fluid raises the intrapericardial pressure which interferes with diastolic filli

Cladogram help, Recent molecular analyses indicate that the artiodactyls, w...

Recent molecular analyses indicate that the artiodactyls, which include hippos and camels, are paraphyletic, whereas cetaceans are monophyletic and cetaceans and hippos form a clad

Explain the lectins or haemagglutinins, Explain the Lectins or Haemagglutin...

Explain the Lectins or Haemagglutinins? These are sugar binding proteins, having the ability to bind and agglutinate red blood cells (RBCs). You will find that they occur in mo

Hydrogen bonds important for making water cohesive liquid, Hydrogen bonds a...

Hydrogen bonds are important for all of the following except:: a) Allowing carbohydrates to dissolve in water b) Stabilizing the three-dimensional shape of proteins. c) Ma

Explain dna or rna segment, DNA or RNA segment tagged with a radioactive mo...

DNA or RNA segment tagged with a radioactive molecule is called: 1. Vector 2. Probe 3. Clone 4. Plasmid Probe

Define the biochemical analysis, Biochemical analysis A basic metabolic...

Biochemical analysis A basic metabolic panel measures sodium, potassium, chloride, bicarbonate, blood urea nitrogen (BUN), magnesium, creatinine, and glucose. It also sometimes

Define nutritive aspects of food constituents, Define Nutritive aspects of ...

Define Nutritive aspects of food constituents and effect of processing and handling? Nutrient stability; effects on nutrients of agricultural practices, handling, processing an

Final energetic products of every round of the krebs cycle, Q. What are the...

Q. What are the final energetic products of every round of the Krebs cycle? And Where is most part of the utile energy at the end of Krebs cycle found? After each round of the

What is the emperical evaluation, What is the Emperical evaluation Emp...

What is the Emperical evaluation Emperical evaluation based on: i) Time elapsed since stage one surgery - Considering the sigma cycle of bone formation in humans, a time pe

Explain leaf primordia, Explain Leaf Primordia ? In contrast to the ro...

Explain Leaf Primordia ? In contrast to the root, which produces branches from meristematic tissue called the pericycle, shoots have areas of meristematic tissues called nodes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd