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Define observation or inference for picric acid test?
1. A mahogany red colour will be seen. The mahogany red colour indicates the presence of reducing sugar. All monosaccharides are reducing sugars and thus will give a positive picric acid test.
2. Maltose and lactose contain a free sugar group and thus can reduce picric acid in alkaline medium. Sucrose does not give this test as it does not have a free sugar group.
3. Starch with very few free sugar groups cannot reduce picric acid. Dextrin being a less complex molecule brings about partial reduction.
Protein denaturizing can be source by temperature variation, pH change, and changes in the concentration of surrounding solutes and by other processes. Most proteins are denatured
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