Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Nitrogen Estimation - Kjeldahl's Method?
Nitrogen in the protein is estimated by a method referred to as Kjeldahl's method. Although over a period of time many other methods have emerged for determination of organic nitrogen, this method still remains an old favourite because it is reliable and has very well standardized procedures. The method involves the digestion of the weighed sample with concentrated sulphuric acid, selenium oxide, potassuim sulphate and copper sulphate in a long necked special digestion flask known as kjeldahl's flask. Potassium sulphate increases the boiling point of the mixture. Since the digestion results in fuming of the sulphuric acid, the procedure has to be carried out in a fume hood and the procedure takes several hours. The sample is digested until the solution in the digestion flask becomes clear after which it is transferred to an appropriate volumetric flask (usually 50 or 100 ml flask) and the volume is made up to the mark with distilled water. Aliquots of this sample are used for distillation in the kjeldahl's apparatus and nitrogen in the protein is estimated. Most proteins contains 16% nitrogen. Thus, percent nitrogen content is multiplied by 6.25 to get crude protein content.
The experiment involves decomposition of ammonium sulphate by a fixed volume of alkali (NaOH). Ammonium sulphate on decomposition releases ammonia which is collected in a flask of boric acid solution Tashiro's indicator. With boric acid ammonia forms a complex 'ammoium borate'. As ammonia is distilled into the boric acid, the Tashiro indicator slowly changes colour from pink to green. This is because the colour of the indicator is pH dependent - it is pink in acidic medium and green in alkaline medium. When the green coloured solution containing the boric-acid-ammonia complex is retitrated against H2SO4, the solution slowly becomes acidic again and the indicator becomes pink. This point when colour changes from green to pink is taken as the end point.
What is Splitin first heart sound ? In complete RBBB, due to delay of tricuspid closure, the split is wide. In complete LBBB, due to delay of mitral closure the S I is single.
What are Taxonomic characters Taxonomic characters Classification is done on the basis of information we have. More information gives better classification. Modern taxonomy
What factors lead to spoilage of fish flesh? A difference in the composition of tissues among different species, climate, procurement and holding practices are amongst few of
What is the main cell organelle involved in cell digestion? What are the properties of that organelle that enable it to do the task? The organelles responsible for intracellula
Q. Why, even though they have an open circulatory system, can flying insects like flies beat their wings with great speed? In insects the circulatory system is open but this sy
could you please give me a data in the form of tabular column based on milk analysis which can be done in home itself?
To obtain the correct Healing Collar the following steps need to be followed: - Determine the si-e of the implant platform.76 Practical Manual - Select the emergence profile
Homology indicates similarity between the two different nucleotide or the amino acid sequences, generally with potential evolutionary importance. It is probably better to use more
Explain the Fructo Oligosaccharides (FOS)? FOS are polymers of fructose, usually attached to an initial glucose molecule. The total number of fructose units range from 2 to 8 a
What are the so-called "good" and "bad" cholesterol? Lipoproteins are complexes made of lipids (triglycerides and cholesterol) and proteins. The lipoproteins present dissimilar
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd