Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Explain the micronutrients requirements during sepsis, Explain the Micronut...

Explain the Micronutrients Requirements during Sepsis Micronutrients: The requirement of almost all vitamins and certain mineral increases due to infection and inflammation. In

Colonial , 1. The level of organization intermediate between unicellular an...

1. The level of organization intermediate between unicellular and the multicellular - organisms are composed of multiple cells but these fail to exhibit specialization of those cel

Explain the stages of alzheimer''s disease, Explain the Stages of alzheimer...

Explain the Stages of alzheimer's disease? Stage I - There is an increased forgetfulness, anxiety and depression. Associated nutrition related changes include difficulty in

Photosynthesis, Photosynthesis Photosynthesis - the process by which p...

Photosynthesis Photosynthesis - the process by which plants utilise energy from sunlight to convert carbon dioxide and water for the synthesis of sugar. This sugar can then be

Animal biodiversity, Discuss why Obelia is considered to be of special inte...

Discuss why Obelia is considered to be of special interest in Zoology as an animal showing an intermediate grade of organization.

How many different gene types are possible, In lab rats, hair color and tex...

In lab rats, hair color and texture are controlled by two genes, the A gene for color and the B gene for texture. in both of these genes, the alleles show no dominance to each othe

What are the flat bones and the long bones, What are the flat bones and the...

What are the flat bones and the long bones? The major bones of the body may be divided as flat or long bones (there are bones not classified into these categories). Such as fla

What is deuterostome. explain in brief., What is Deuterostome. Explain in b...

What is Deuterostome. Explain in brief. Phyla, including the Chordata and Echinodermata which share common characteristics of blastopore-not forming the mouth, radial indetermi

Change of health status over time, Change of Health Status Over Time Hea...

Change of Health Status Over Time Health of a nation can be gauged from the available information on death.Disaggregated data by causes of death is more reflective of the status

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd